Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09430 and PS417_09435 are separated by 201 nucleotides PS417_09435 and PS417_09440 are separated by 243 nucleotides PS417_09440 and PS417_09445 are separated by 101 nucleotides PS417_09445 and PS417_09450 overlap by 12 nucleotides
PS417_09430: PS417_09430 - alpha/beta hydrolase, at 2,043,561 to 2,044,436
_09430
PS417_09435: PS417_09435 - glyoxalase, at 2,044,638 to 2,045,096
_09435
PS417_09440: PS417_09440 - MerR family transcriptional regulator, at 2,045,340 to 2,045,795
_09440
PS417_09445: PS417_09445 - antibiotic biosynthesis monooxygenase, at 2,045,897 to 2,046,262
_09445
PS417_09450: PS417_09450 - Fis family transcriptional regulator, at 2,046,251 to 2,047,354
_09450
Position (kb)
2045
2046 Strain fitness (log2 ratio)
-2
-1
0
1 at 2044.530 kb on - strand at 2044.573 kb on - strand at 2044.618 kb on + strand at 2044.618 kb on + strand at 2044.701 kb on - strand at 2044.701 kb on - strand at 2044.843 kb on + strand, within PS417_09435 at 2044.844 kb on - strand, within PS417_09435 at 2044.844 kb on - strand, within PS417_09435 at 2045.011 kb on + strand, within PS417_09435 at 2045.050 kb on + strand, within PS417_09435 at 2045.051 kb on - strand at 2045.133 kb on + strand at 2045.134 kb on - strand at 2045.134 kb on - strand at 2045.134 kb on - strand at 2045.134 kb on - strand at 2045.138 kb on - strand at 2045.138 kb on - strand at 2045.138 kb on - strand at 2045.140 kb on - strand at 2045.330 kb on + strand at 2045.456 kb on + strand, within PS417_09440 at 2045.722 kb on + strand, within PS417_09440 at 2045.876 kb on + strand at 2045.904 kb on + strand at 2045.905 kb on - strand at 2045.905 kb on - strand at 2046.077 kb on + strand, within PS417_09445 at 2046.078 kb on - strand, within PS417_09445 at 2046.260 kb on + strand at 2046.569 kb on + strand, within PS417_09450 at 2046.570 kb on - strand, within PS417_09450 at 2046.660 kb on + strand, within PS417_09450 at 2046.660 kb on + strand, within PS417_09450 at 2046.661 kb on - strand, within PS417_09450 at 2046.661 kb on - strand, within PS417_09450 at 2046.661 kb on - strand, within PS417_09450 at 2046.726 kb on + strand, within PS417_09450
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,044,530 - -1.6 2,044,573 - +0.6 2,044,618 + -0.1 2,044,618 + -0.4 2,044,701 - +0.2 2,044,701 - +1.7 2,044,843 + PS417_09435 0.45 +0.5 2,044,844 - PS417_09435 0.45 -0.7 2,044,844 - PS417_09435 0.45 -0.2 2,045,011 + PS417_09435 0.81 -0.2 2,045,050 + PS417_09435 0.90 +0.2 2,045,051 - -0.8 2,045,133 + -1.0 2,045,134 - -0.3 2,045,134 - -0.8 2,045,134 - +0.0 2,045,134 - +0.8 2,045,138 - +0.6 2,045,138 - -0.0 2,045,138 - +0.5 2,045,140 - +1.6 2,045,330 + +1.3 2,045,456 + PS417_09440 0.25 -0.2 2,045,722 + PS417_09440 0.84 -1.7 2,045,876 + -0.2 2,045,904 + +0.4 2,045,905 - +0.3 2,045,905 - +1.0 2,046,077 + PS417_09445 0.49 +0.5 2,046,078 - PS417_09445 0.49 -1.2 2,046,260 + +1.0 2,046,569 + PS417_09450 0.29 -0.2 2,046,570 - PS417_09450 0.29 -0.4 2,046,660 + PS417_09450 0.37 -2.5 2,046,660 + PS417_09450 0.37 +0.2 2,046,661 - PS417_09450 0.37 -0.6 2,046,661 - PS417_09450 0.37 +0.7 2,046,661 - PS417_09450 0.37 -0.6 2,046,726 + PS417_09450 0.43 -0.2
Or see this region's nucleotide sequence