Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09340 and PS417_09345 overlap by 4 nucleotides PS417_09345 and PS417_09350 overlap by 14 nucleotides PS417_09350 and PS417_09355 overlap by 4 nucleotides PS417_09355 and PS417_09360 are separated by 179 nucleotides
PS417_09340: PS417_09340 - spermidine/putrescine ABC transporter ATP-binding protein, at 2,026,788 to 2,027,777
_09340
PS417_09345: PS417_09345 - ABC transporter permease, at 2,027,774 to 2,028,574
_09345
PS417_09350: PS417_09350 - ABC transporter permease, at 2,028,561 to 2,029,397
_09350
PS417_09355: PS417_09355 - hypothetical protein, at 2,029,394 to 2,030,200
_09355
PS417_09360: PS417_09360 - ABC transporter substrate-binding protein, at 2,030,380 to 2,031,456
_09360
Position (kb)
2028
2029
2030 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2027.838 kb on + strand at 2028.144 kb on + strand, within PS417_09345 at 2028.144 kb on + strand, within PS417_09345 at 2028.198 kb on + strand, within PS417_09345 at 2028.199 kb on - strand, within PS417_09345 at 2028.295 kb on - strand, within PS417_09345 at 2028.295 kb on - strand, within PS417_09345 at 2028.516 kb on - strand at 2028.615 kb on + strand at 2028.742 kb on + strand, within PS417_09350 at 2028.743 kb on - strand, within PS417_09350 at 2028.943 kb on + strand, within PS417_09350 at 2028.944 kb on - strand, within PS417_09350 at 2028.944 kb on - strand, within PS417_09350 at 2028.944 kb on - strand, within PS417_09350 at 2028.947 kb on + strand, within PS417_09350 at 2028.947 kb on + strand, within PS417_09350 at 2028.947 kb on + strand, within PS417_09350 at 2028.947 kb on + strand, within PS417_09350 at 2028.948 kb on - strand, within PS417_09350 at 2028.948 kb on - strand, within PS417_09350 at 2028.949 kb on + strand at 2028.949 kb on + strand, within PS417_09350 at 2028.950 kb on - strand, within PS417_09350 at 2028.950 kb on - strand, within PS417_09350 at 2028.950 kb on - strand, within PS417_09350 at 2028.977 kb on - strand, within PS417_09350 at 2029.192 kb on + strand, within PS417_09350 at 2029.192 kb on + strand, within PS417_09350 at 2029.386 kb on - strand at 2029.636 kb on - strand, within PS417_09355 at 2029.677 kb on + strand at 2030.353 kb on + strand at 2030.381 kb on + strand at 2030.381 kb on + strand at 2030.392 kb on + strand at 2030.393 kb on - strand at 2030.395 kb on + strand at 2030.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,027,838 + -0.6 2,028,144 + PS417_09345 0.46 -0.6 2,028,144 + PS417_09345 0.46 -0.5 2,028,198 + PS417_09345 0.53 +0.1 2,028,199 - PS417_09345 0.53 +0.3 2,028,295 - PS417_09345 0.65 -0.8 2,028,295 - PS417_09345 0.65 -1.1 2,028,516 - +0.2 2,028,615 + -1.1 2,028,742 + PS417_09350 0.22 -0.4 2,028,743 - PS417_09350 0.22 -2.0 2,028,943 + PS417_09350 0.46 -1.4 2,028,944 - PS417_09350 0.46 +0.0 2,028,944 - PS417_09350 0.46 -1.7 2,028,944 - PS417_09350 0.46 +0.5 2,028,947 + PS417_09350 0.46 -1.1 2,028,947 + PS417_09350 0.46 -1.5 2,028,947 + PS417_09350 0.46 -0.3 2,028,947 + PS417_09350 0.46 +0.1 2,028,948 - PS417_09350 0.46 +0.5 2,028,948 - PS417_09350 0.46 +0.3 2,028,949 + -1.1 2,028,949 + PS417_09350 0.46 -1.4 2,028,950 - PS417_09350 0.46 -0.3 2,028,950 - PS417_09350 0.46 -1.3 2,028,950 - PS417_09350 0.46 -0.3 2,028,977 - PS417_09350 0.50 -1.0 2,029,192 + PS417_09350 0.75 +0.2 2,029,192 + PS417_09350 0.75 -0.7 2,029,386 - -1.6 2,029,636 - PS417_09355 0.30 -2.5 2,029,677 + -0.8 2,030,353 + -0.5 2,030,381 + +0.6 2,030,381 + +3.3 2,030,392 + +0.3 2,030,393 - +1.5 2,030,395 + +0.6 2,030,396 - +0.4
Or see this region's nucleotide sequence