Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09330 and PS417_09335 are separated by 144 nucleotides PS417_09335 and PS417_09340 are separated by 30 nucleotides PS417_09340 and PS417_09345 overlap by 4 nucleotides PS417_09345 and PS417_09350 overlap by 14 nucleotides
PS417_09330: PS417_09330 - NAD(P)H-quinone oxidoreductase, at 2,024,997 to 2,025,959
_09330
PS417_09335: PS417_09335 - HAD family hydrolase, at 2,026,104 to 2,026,757
_09335
PS417_09340: PS417_09340 - spermidine/putrescine ABC transporter ATP-binding protein, at 2,026,788 to 2,027,777
_09340
PS417_09345: PS417_09345 - ABC transporter permease, at 2,027,774 to 2,028,574
_09345
PS417_09350: PS417_09350 - ABC transporter permease, at 2,028,561 to 2,029,397
_09350
Position (kb)
2026
2027
2028 Strain fitness (log2 ratio)
-2
-1
0
1 at 2026.012 kb on + strand at 2026.334 kb on - strand, within PS417_09335 at 2026.794 kb on + strand at 2027.068 kb on + strand, within PS417_09340 at 2027.068 kb on + strand, within PS417_09340 at 2027.069 kb on - strand, within PS417_09340 at 2027.094 kb on + strand, within PS417_09340 at 2027.094 kb on + strand, within PS417_09340 at 2027.095 kb on - strand, within PS417_09340 at 2027.113 kb on + strand, within PS417_09340 at 2027.838 kb on + strand at 2028.144 kb on + strand, within PS417_09345 at 2028.144 kb on + strand, within PS417_09345 at 2028.198 kb on + strand, within PS417_09345 at 2028.199 kb on - strand, within PS417_09345 at 2028.295 kb on - strand, within PS417_09345 at 2028.295 kb on - strand, within PS417_09345 at 2028.516 kb on - strand at 2028.615 kb on + strand at 2028.742 kb on + strand, within PS417_09350 at 2028.743 kb on - strand, within PS417_09350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,026,012 + -0.9 2,026,334 - PS417_09335 0.35 -1.0 2,026,794 + +0.7 2,027,068 + PS417_09340 0.28 -0.9 2,027,068 + PS417_09340 0.28 -0.7 2,027,069 - PS417_09340 0.28 +0.1 2,027,094 + PS417_09340 0.31 +0.2 2,027,094 + PS417_09340 0.31 +1.7 2,027,095 - PS417_09340 0.31 +0.2 2,027,113 + PS417_09340 0.33 -0.8 2,027,838 + -0.6 2,028,144 + PS417_09345 0.46 -0.6 2,028,144 + PS417_09345 0.46 -0.5 2,028,198 + PS417_09345 0.53 +0.1 2,028,199 - PS417_09345 0.53 +0.3 2,028,295 - PS417_09345 0.65 -0.8 2,028,295 - PS417_09345 0.65 -1.1 2,028,516 - +0.2 2,028,615 + -1.1 2,028,742 + PS417_09350 0.22 -0.4 2,028,743 - PS417_09350 0.22 -2.0
Or see this region's nucleotide sequence