Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08715 and PS417_08720 overlap by 4 nucleotides PS417_08720 and PS417_08725 overlap by 4 nucleotides PS417_08725 and PS417_08730 overlap by 8 nucleotides PS417_08730 and PS417_08735 are separated by 16 nucleotides PS417_08735 and PS417_08740 are separated by 278 nucleotides
PS417_08715: PS417_08715 - cytochrome C biogenesis protein CcmF, at 1,898,088 to 1,900,076
_08715
PS417_08720: PS417_08720 - thiol:disulfide interchange protein, at 1,900,073 to 1,900,609
_08720
PS417_08725: PS417_08725 - cytochrome C, at 1,900,606 to 1,901,079
_08725
PS417_08730: PS417_08730 - cytochrome C, at 1,901,072 to 1,902,259
_08730
PS417_08735: PS417_08735 - hypothetical protein, at 1,902,276 to 1,902,671
_08735
PS417_08740: PS417_08740 - ribose-phosphate pyrophosphokinase, at 1,902,950 to 1,903,522
_08740
Position (kb)
1901
1902
1903 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1901.550 kb on - strand, within PS417_08730 at 1901.988 kb on + strand, within PS417_08730 at 1901.989 kb on - strand, within PS417_08730 at 1902.254 kb on - strand at 1902.516 kb on + strand, within PS417_08735 at 1902.516 kb on + strand, within PS417_08735 at 1902.516 kb on + strand, within PS417_08735 at 1902.516 kb on + strand, within PS417_08735 at 1902.547 kb on - strand, within PS417_08735 at 1902.547 kb on - strand, within PS417_08735 at 1902.561 kb on + strand, within PS417_08735 at 1902.561 kb on + strand, within PS417_08735 at 1902.562 kb on - strand, within PS417_08735 at 1902.562 kb on - strand, within PS417_08735 at 1902.843 kb on - strand at 1902.843 kb on - strand at 1902.856 kb on + strand at 1902.857 kb on - strand at 1902.968 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,901,550 - PS417_08730 0.40 -1.2 1,901,988 + PS417_08730 0.77 -0.4 1,901,989 - PS417_08730 0.77 -0.7 1,902,254 - +2.8 1,902,516 + PS417_08735 0.61 +0.8 1,902,516 + PS417_08735 0.61 +0.9 1,902,516 + PS417_08735 0.61 +2.2 1,902,516 + PS417_08735 0.61 +0.4 1,902,547 - PS417_08735 0.68 +0.0 1,902,547 - PS417_08735 0.68 -0.9 1,902,561 + PS417_08735 0.72 +0.4 1,902,561 + PS417_08735 0.72 +0.8 1,902,562 - PS417_08735 0.72 +0.1 1,902,562 - PS417_08735 0.72 -0.9 1,902,843 - +0.3 1,902,843 - -0.8 1,902,856 + +0.9 1,902,857 - +0.4 1,902,968 + -1.9
Or see this region's nucleotide sequence