Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08525 and PS417_08530 are separated by 123 nucleotides PS417_08530 and PS417_08535 are separated by 118 nucleotides
PS417_08525: PS417_08525 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 1,861,670 to 1,863,016
_08525
PS417_08530: PS417_08530 - spermidine synthase, at 1,863,140 to 1,863,895
_08530
PS417_08535: PS417_08535 - enoyl-CoA hydratase, at 1,864,014 to 1,864,805
_08535
Position (kb)
1863
1864 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1862.256 kb on + strand, within PS417_08525 at 1862.298 kb on + strand, within PS417_08525 at 1862.298 kb on + strand, within PS417_08525 at 1862.299 kb on - strand at 1862.422 kb on - strand, within PS417_08525 at 1862.544 kb on + strand, within PS417_08525 at 1862.545 kb on - strand, within PS417_08525 at 1862.756 kb on + strand, within PS417_08525 at 1862.757 kb on - strand, within PS417_08525 at 1862.757 kb on - strand, within PS417_08525 at 1862.927 kb on + strand at 1862.928 kb on - strand at 1863.057 kb on + strand at 1863.058 kb on - strand at 1863.068 kb on - strand at 1863.068 kb on - strand at 1863.068 kb on - strand at 1863.264 kb on + strand, within PS417_08530 at 1863.264 kb on + strand, within PS417_08530 at 1863.299 kb on + strand, within PS417_08530 at 1863.300 kb on - strand, within PS417_08530 at 1863.498 kb on - strand, within PS417_08530 at 1863.498 kb on - strand, within PS417_08530 at 1863.498 kb on - strand, within PS417_08530 at 1863.498 kb on - strand, within PS417_08530 at 1863.500 kb on - strand, within PS417_08530 at 1863.569 kb on + strand, within PS417_08530 at 1863.569 kb on + strand, within PS417_08530 at 1863.570 kb on - strand, within PS417_08530 at 1863.719 kb on + strand, within PS417_08530 at 1863.795 kb on + strand, within PS417_08530 at 1863.894 kb on - strand at 1863.904 kb on - strand at 1863.907 kb on + strand at 1863.908 kb on - strand at 1863.908 kb on - strand at 1863.908 kb on - strand at 1863.908 kb on - strand at 1864.020 kb on - strand at 1864.176 kb on + strand, within PS417_08535 at 1864.176 kb on + strand, within PS417_08535 at 1864.177 kb on - strand, within PS417_08535 at 1864.746 kb on + strand at 1864.790 kb on + strand at 1864.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,862,256 + PS417_08525 0.44 -0.8 1,862,298 + PS417_08525 0.47 +0.6 1,862,298 + PS417_08525 0.47 +1.7 1,862,299 - -0.9 1,862,422 - PS417_08525 0.56 -1.1 1,862,544 + PS417_08525 0.65 +0.3 1,862,545 - PS417_08525 0.65 +0.6 1,862,756 + PS417_08525 0.81 -0.9 1,862,757 - PS417_08525 0.81 -0.4 1,862,757 - PS417_08525 0.81 -0.3 1,862,927 + +0.1 1,862,928 - -0.1 1,863,057 + +0.0 1,863,058 - -1.1 1,863,068 - -1.3 1,863,068 - -0.6 1,863,068 - -0.2 1,863,264 + PS417_08530 0.16 +0.1 1,863,264 + PS417_08530 0.16 +0.8 1,863,299 + PS417_08530 0.21 -1.7 1,863,300 - PS417_08530 0.21 +0.0 1,863,498 - PS417_08530 0.47 +2.8 1,863,498 - PS417_08530 0.47 -0.5 1,863,498 - PS417_08530 0.47 -0.4 1,863,498 - PS417_08530 0.47 +0.6 1,863,500 - PS417_08530 0.48 -0.0 1,863,569 + PS417_08530 0.57 +0.5 1,863,569 + PS417_08530 0.57 -1.8 1,863,570 - PS417_08530 0.57 -1.4 1,863,719 + PS417_08530 0.77 -1.4 1,863,795 + PS417_08530 0.87 -0.3 1,863,894 - -1.4 1,863,904 - +0.4 1,863,907 + +0.1 1,863,908 - -1.1 1,863,908 - -0.1 1,863,908 - +0.2 1,863,908 - +0.2 1,864,020 - -1.1 1,864,176 + PS417_08535 0.20 -0.5 1,864,176 + PS417_08535 0.20 -0.4 1,864,177 - PS417_08535 0.21 -0.8 1,864,746 + -0.9 1,864,790 + +0.8 1,864,871 + +0.2
Or see this region's nucleotide sequence