Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07340 and PS417_07345 are separated by 54 nucleotides PS417_07345 and PS417_07350 are separated by 30 nucleotides PS417_07350 and PS417_07355 are separated by 112 nucleotides
PS417_07340: PS417_07340 - calcium channel protein, at 1,618,429 to 1,619,268
_07340
PS417_07345: PS417_07345 - cyanate hydratase, at 1,619,323 to 1,619,793
_07345
PS417_07350: PS417_07350 - carbonic anhydrase, at 1,619,824 to 1,620,456
_07350
PS417_07355: PS417_07355 - transcriptional regulator, at 1,620,569 to 1,621,435
_07355
Position (kb)
1619
1620 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1618.356 kb on + strand at 1618.360 kb on - strand at 1618.367 kb on + strand at 1618.367 kb on + strand at 1618.368 kb on - strand at 1618.412 kb on + strand at 1618.412 kb on + strand at 1618.428 kb on - strand at 1618.428 kb on - strand at 1618.430 kb on + strand at 1618.439 kb on + strand at 1618.440 kb on - strand at 1618.440 kb on - strand at 1618.442 kb on + strand at 1618.479 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.481 kb on + strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.482 kb on - strand at 1618.529 kb on + strand, within PS417_07340 at 1618.529 kb on + strand at 1618.530 kb on - strand, within PS417_07340 at 1618.535 kb on + strand, within PS417_07340 at 1618.535 kb on + strand, within PS417_07340 at 1618.536 kb on - strand, within PS417_07340 at 1618.658 kb on + strand, within PS417_07340 at 1618.659 kb on - strand, within PS417_07340 at 1618.659 kb on - strand, within PS417_07340 at 1618.659 kb on - strand, within PS417_07340 at 1618.659 kb on - strand, within PS417_07340 at 1618.691 kb on + strand, within PS417_07340 at 1618.691 kb on + strand, within PS417_07340 at 1618.691 kb on + strand, within PS417_07340 at 1618.826 kb on + strand, within PS417_07340 at 1618.827 kb on - strand, within PS417_07340 at 1618.827 kb on - strand, within PS417_07340 at 1618.862 kb on + strand, within PS417_07340 at 1619.076 kb on - strand, within PS417_07340 at 1619.076 kb on - strand, within PS417_07340 at 1619.144 kb on + strand, within PS417_07340 at 1619.145 kb on - strand, within PS417_07340 at 1619.147 kb on + strand, within PS417_07340 at 1619.260 kb on + strand at 1619.261 kb on - strand at 1619.261 kb on - strand at 1619.261 kb on - strand at 1619.345 kb on + strand at 1619.345 kb on + strand at 1619.346 kb on - strand at 1619.510 kb on + strand, within PS417_07345 at 1619.913 kb on - strand, within PS417_07350 at 1619.913 kb on - strand, within PS417_07350 at 1620.510 kb on - strand at 1620.552 kb on + strand at 1620.553 kb on - strand at 1620.758 kb on + strand, within PS417_07355 at 1620.758 kb on + strand, within PS417_07355 at 1620.758 kb on + strand, within PS417_07355 at 1620.758 kb on + strand, within PS417_07355 at 1620.758 kb on + strand, within PS417_07355 at 1620.759 kb on - strand, within PS417_07355 at 1620.759 kb on - strand, within PS417_07355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,618,356 + -0.6 1,618,360 - -0.6 1,618,367 + +0.1 1,618,367 + +0.1 1,618,368 - -3.0 1,618,412 + -0.1 1,618,412 + -0.9 1,618,428 - -0.2 1,618,428 - -0.7 1,618,430 + -1.3 1,618,439 + -1.0 1,618,440 - -1.0 1,618,440 - +1.0 1,618,442 + +2.7 1,618,479 + -0.2 1,618,481 + -0.3 1,618,481 + -0.7 1,618,481 + -0.8 1,618,481 + -0.1 1,618,481 + +3.7 1,618,481 + -3.0 1,618,481 + -1.4 1,618,481 + -1.1 1,618,481 + -0.6 1,618,482 - +0.3 1,618,482 - -0.5 1,618,482 - +0.1 1,618,482 - -0.1 1,618,482 - -3.3 1,618,482 - +0.3 1,618,482 - -0.5 1,618,482 - -0.3 1,618,529 + PS417_07340 0.12 +0.4 1,618,529 + +0.1 1,618,530 - PS417_07340 0.12 -1.7 1,618,535 + PS417_07340 0.13 -0.7 1,618,535 + PS417_07340 0.13 -0.2 1,618,536 - PS417_07340 0.13 -0.6 1,618,658 + PS417_07340 0.27 -0.3 1,618,659 - PS417_07340 0.27 +0.2 1,618,659 - PS417_07340 0.27 -0.2 1,618,659 - PS417_07340 0.27 +0.3 1,618,659 - PS417_07340 0.27 +0.6 1,618,691 + PS417_07340 0.31 +0.0 1,618,691 + PS417_07340 0.31 +0.2 1,618,691 + PS417_07340 0.31 +0.5 1,618,826 + PS417_07340 0.47 -1.4 1,618,827 - PS417_07340 0.47 -0.2 1,618,827 - PS417_07340 0.47 -1.1 1,618,862 + PS417_07340 0.52 -0.9 1,619,076 - PS417_07340 0.77 -0.9 1,619,076 - PS417_07340 0.77 -0.4 1,619,144 + PS417_07340 0.85 -0.0 1,619,145 - PS417_07340 0.85 -1.0 1,619,147 + PS417_07340 0.85 -0.1 1,619,260 + -0.1 1,619,261 - -0.5 1,619,261 - +0.1 1,619,261 - -1.5 1,619,345 + -0.2 1,619,345 + -0.2 1,619,346 - +0.2 1,619,510 + PS417_07345 0.40 -1.3 1,619,913 - PS417_07350 0.14 +2.3 1,619,913 - PS417_07350 0.14 -0.9 1,620,510 - +1.6 1,620,552 + -1.0 1,620,553 - -0.6 1,620,758 + PS417_07355 0.22 -0.1 1,620,758 + PS417_07355 0.22 +0.6 1,620,758 + PS417_07355 0.22 +0.3 1,620,758 + PS417_07355 0.22 +0.2 1,620,758 + PS417_07355 0.22 -0.7 1,620,759 - PS417_07355 0.22 -0.0 1,620,759 - PS417_07355 0.22 +0.1
Or see this region's nucleotide sequence