Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05520 and PS417_05525 overlap by 11 nucleotides PS417_05525 and PS417_05530 are separated by 34 nucleotides PS417_05530 and PS417_05535 overlap by 1 nucleotides
PS417_05520: PS417_05520 - histidine kinase, at 1,241,445 to 1,243,214
_05520
PS417_05525: PS417_05525 - Fis family transcriptional regulator, at 1,243,204 to 1,244,601
_05525
PS417_05530: PS417_05530 - membrane protein, at 1,244,636 to 1,244,974
_05530
PS417_05535: PS417_05535 - Fis family transcriptional regulator, at 1,244,974 to 1,246,323
_05535
Position (kb)
1243
1244
1245 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1242.461 kb on + strand, within PS417_05520 at 1242.473 kb on + strand, within PS417_05520 at 1242.474 kb on - strand, within PS417_05520 at 1242.474 kb on - strand, within PS417_05520 at 1242.748 kb on + strand, within PS417_05520 at 1242.749 kb on - strand, within PS417_05520 at 1242.749 kb on - strand, within PS417_05520 at 1242.815 kb on + strand, within PS417_05520 at 1242.815 kb on + strand, within PS417_05520 at 1242.815 kb on + strand, within PS417_05520 at 1242.816 kb on - strand, within PS417_05520 at 1242.816 kb on - strand, within PS417_05520 at 1242.818 kb on + strand, within PS417_05520 at 1242.861 kb on + strand, within PS417_05520 at 1243.167 kb on + strand at 1243.167 kb on + strand at 1243.167 kb on + strand at 1243.212 kb on + strand at 1243.495 kb on + strand, within PS417_05525 at 1243.496 kb on - strand, within PS417_05525 at 1243.502 kb on - strand, within PS417_05525 at 1244.105 kb on - strand, within PS417_05525 at 1244.303 kb on + strand, within PS417_05525 at 1244.304 kb on - strand, within PS417_05525 at 1244.470 kb on + strand at 1244.470 kb on + strand at 1244.471 kb on - strand at 1244.475 kb on - strand at 1244.475 kb on - strand at 1244.745 kb on + strand at 1244.746 kb on - strand, within PS417_05530 at 1244.746 kb on - strand, within PS417_05530 at 1244.760 kb on + strand, within PS417_05530 at 1244.844 kb on + strand, within PS417_05530 at 1244.845 kb on - strand, within PS417_05530 at 1244.895 kb on + strand, within PS417_05530 at 1244.896 kb on - strand, within PS417_05530 at 1244.896 kb on - strand, within PS417_05530 at 1244.898 kb on + strand, within PS417_05530 at 1245.003 kb on + strand at 1245.393 kb on - strand at 1245.393 kb on - strand, within PS417_05535 at 1245.393 kb on - strand, within PS417_05535 at 1245.529 kb on + strand, within PS417_05535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,242,461 + PS417_05520 0.57 -2.1 1,242,473 + PS417_05520 0.58 +1.1 1,242,474 - PS417_05520 0.58 -0.3 1,242,474 - PS417_05520 0.58 +2.2 1,242,748 + PS417_05520 0.74 -2.7 1,242,749 - PS417_05520 0.74 -0.3 1,242,749 - PS417_05520 0.74 -1.0 1,242,815 + PS417_05520 0.77 +1.1 1,242,815 + PS417_05520 0.77 +0.1 1,242,815 + PS417_05520 0.77 +1.3 1,242,816 - PS417_05520 0.77 -1.9 1,242,816 - PS417_05520 0.77 -2.4 1,242,818 + PS417_05520 0.78 +0.1 1,242,861 + PS417_05520 0.80 -0.6 1,243,167 + +2.0 1,243,167 + +1.0 1,243,167 + +1.5 1,243,212 + -3.5 1,243,495 + PS417_05525 0.21 -0.8 1,243,496 - PS417_05525 0.21 -2.5 1,243,502 - PS417_05525 0.21 -1.0 1,244,105 - PS417_05525 0.64 -1.3 1,244,303 + PS417_05525 0.79 -0.5 1,244,304 - PS417_05525 0.79 -1.6 1,244,470 + -1.5 1,244,470 + -3.9 1,244,471 - -0.6 1,244,475 - +0.5 1,244,475 - -1.2 1,244,745 + -0.9 1,244,746 - PS417_05530 0.32 -0.6 1,244,746 - PS417_05530 0.32 +0.8 1,244,760 + PS417_05530 0.37 -0.3 1,244,844 + PS417_05530 0.61 -0.1 1,244,845 - PS417_05530 0.62 +1.5 1,244,895 + PS417_05530 0.76 -0.6 1,244,896 - PS417_05530 0.77 -0.1 1,244,896 - PS417_05530 0.77 +0.9 1,244,898 + PS417_05530 0.77 -0.1 1,245,003 + +0.0 1,245,393 - -0.9 1,245,393 - PS417_05535 0.31 -0.0 1,245,393 - PS417_05535 0.31 -2.1 1,245,529 + PS417_05535 0.41 +0.7
Or see this region's nucleotide sequence