Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05505 and PS417_05510 overlap by 4 nucleotides PS417_05510 and PS417_05515 are separated by 31 nucleotides
PS417_05505: PS417_05505 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 1,237,948 to 1,239,342
_05505
PS417_05510: PS417_05510 - alpha/beta hydrolase, at 1,239,339 to 1,240,289
_05510
PS417_05515: PS417_05515 - hypothetical protein, at 1,240,321 to 1,241,445
_05515
Position (kb)
1239
1240
1241 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1238.427 kb on + strand, within PS417_05505 at 1238.428 kb on - strand, within PS417_05505 at 1238.584 kb on + strand, within PS417_05505 at 1238.623 kb on + strand, within PS417_05505 at 1238.624 kb on - strand, within PS417_05505 at 1238.636 kb on - strand, within PS417_05505 at 1238.666 kb on - strand at 1238.761 kb on + strand, within PS417_05505 at 1239.155 kb on - strand, within PS417_05505 at 1239.155 kb on - strand, within PS417_05505 at 1239.155 kb on - strand, within PS417_05505 at 1239.155 kb on - strand, within PS417_05505 at 1239.232 kb on + strand at 1239.350 kb on - strand at 1239.495 kb on + strand, within PS417_05510 at 1239.496 kb on - strand, within PS417_05510 at 1239.657 kb on + strand, within PS417_05510 at 1239.657 kb on + strand, within PS417_05510 at 1239.657 kb on + strand, within PS417_05510 at 1239.657 kb on + strand, within PS417_05510 at 1239.657 kb on + strand, within PS417_05510 at 1239.658 kb on - strand, within PS417_05510 at 1239.703 kb on - strand, within PS417_05510 at 1239.720 kb on + strand, within PS417_05510 at 1239.882 kb on - strand, within PS417_05510 at 1240.035 kb on + strand, within PS417_05510 at 1240.414 kb on + strand at 1240.414 kb on + strand at 1240.414 kb on + strand at 1240.415 kb on - strand at 1240.415 kb on - strand at 1240.609 kb on + strand, within PS417_05515 at 1240.842 kb on - strand, within PS417_05515 at 1240.873 kb on + strand, within PS417_05515 at 1240.873 kb on + strand, within PS417_05515 at 1240.874 kb on - strand, within PS417_05515 at 1240.874 kb on - strand, within PS417_05515 at 1240.874 kb on - strand, within PS417_05515 at 1240.874 kb on - strand, within PS417_05515 at 1240.874 kb on - strand, within PS417_05515 at 1240.874 kb on - strand, within PS417_05515 at 1241.099 kb on - strand, within PS417_05515 at 1241.201 kb on - strand, within PS417_05515 at 1241.201 kb on - strand, within PS417_05515 at 1241.244 kb on + strand, within PS417_05515 at 1241.244 kb on + strand, within PS417_05515 at 1241.245 kb on - strand, within PS417_05515 at 1241.245 kb on - strand, within PS417_05515 at 1241.245 kb on - strand, within PS417_05515 at 1241.257 kb on - strand, within PS417_05515 at 1241.266 kb on + strand, within PS417_05515 at 1241.266 kb on + strand, within PS417_05515 at 1241.266 kb on + strand, within PS417_05515 at 1241.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,238,427 + PS417_05505 0.34 -2.6 1,238,428 - PS417_05505 0.34 -0.3 1,238,584 + PS417_05505 0.46 +1.7 1,238,623 + PS417_05505 0.48 -1.6 1,238,624 - PS417_05505 0.48 -0.1 1,238,636 - PS417_05505 0.49 -0.9 1,238,666 - -0.6 1,238,761 + PS417_05505 0.58 -0.3 1,239,155 - PS417_05505 0.87 -2.6 1,239,155 - PS417_05505 0.87 -1.7 1,239,155 - PS417_05505 0.87 -3.3 1,239,155 - PS417_05505 0.87 +0.1 1,239,232 + -2.2 1,239,350 - -3.8 1,239,495 + PS417_05510 0.16 -0.8 1,239,496 - PS417_05510 0.17 +0.4 1,239,657 + PS417_05510 0.33 -2.2 1,239,657 + PS417_05510 0.33 -1.3 1,239,657 + PS417_05510 0.33 -1.7 1,239,657 + PS417_05510 0.33 -1.2 1,239,657 + PS417_05510 0.33 -2.1 1,239,658 - PS417_05510 0.34 -1.9 1,239,703 - PS417_05510 0.38 -2.5 1,239,720 + PS417_05510 0.40 -1.6 1,239,882 - PS417_05510 0.57 -0.3 1,240,035 + PS417_05510 0.73 -2.9 1,240,414 + -2.7 1,240,414 + -1.4 1,240,414 + -1.7 1,240,415 - -1.2 1,240,415 - -1.0 1,240,609 + PS417_05515 0.26 -2.5 1,240,842 - PS417_05515 0.46 -1.2 1,240,873 + PS417_05515 0.49 -4.2 1,240,873 + PS417_05515 0.49 -2.5 1,240,874 - PS417_05515 0.49 -2.7 1,240,874 - PS417_05515 0.49 -0.6 1,240,874 - PS417_05515 0.49 -2.5 1,240,874 - PS417_05515 0.49 -0.0 1,240,874 - PS417_05515 0.49 -0.8 1,240,874 - PS417_05515 0.49 +0.3 1,241,099 - PS417_05515 0.69 +0.2 1,241,201 - PS417_05515 0.78 -0.5 1,241,201 - PS417_05515 0.78 -0.4 1,241,244 + PS417_05515 0.82 -2.6 1,241,244 + PS417_05515 0.82 +1.7 1,241,245 - PS417_05515 0.82 +0.1 1,241,245 - PS417_05515 0.82 -1.1 1,241,245 - PS417_05515 0.82 +0.5 1,241,257 - PS417_05515 0.83 -0.0 1,241,266 + PS417_05515 0.84 -1.4 1,241,266 + PS417_05515 0.84 -2.0 1,241,266 + PS417_05515 0.84 -0.6 1,241,267 - +1.7
Or see this region's nucleotide sequence