Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03990 and PS417_03995 are separated by 204 nucleotides PS417_03995 and PS417_04000 are separated by 2 nucleotides PS417_04000 and PS417_04005 are separated by 49 nucleotides
PS417_03990: PS417_03990 - ligand-gated channel protein, at 895,376 to 897,661
_03990
PS417_03995: PS417_03995 - Fe(II)-dependent oxygenase, at 897,866 to 898,546
_03995
PS417_04000: PS417_04000 - hypothetical protein, at 898,549 to 899,301
_04000
PS417_04005: PS417_04005 - ornithine decarboxylase, at 899,351 to 900,514
_04005
Position (kb) 898
899
900 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 897.639 kb on - strand at 897.698 kb on + strand at 898.075 kb on + strand, within PS417_03995 at 898.075 kb on + strand, within PS417_03995 at 898.075 kb on + strand, within PS417_03995 at 898.125 kb on - strand, within PS417_03995 at 898.125 kb on - strand, within PS417_03995 at 898.374 kb on - strand, within PS417_03995 at 898.511 kb on + strand at 898.512 kb on - strand at 898.512 kb on - strand at 898.559 kb on + strand at 898.560 kb on - strand at 898.788 kb on + strand, within PS417_04000 at 898.855 kb on + strand, within PS417_04000 at 898.894 kb on + strand, within PS417_04000 at 898.894 kb on + strand, within PS417_04000 at 898.895 kb on - strand, within PS417_04000 at 898.895 kb on - strand, within PS417_04000 at 898.895 kb on - strand, within PS417_04000 at 898.895 kb on - strand, within PS417_04000 at 898.895 kb on - strand, within PS417_04000 at 898.900 kb on + strand, within PS417_04000 at 898.900 kb on + strand, within PS417_04000 at 898.900 kb on + strand, within PS417_04000 at 898.901 kb on - strand, within PS417_04000 at 898.963 kb on + strand, within PS417_04000 at 899.117 kb on - strand, within PS417_04000 at 899.270 kb on - strand at 899.270 kb on - strand at 899.300 kb on - strand at 899.320 kb on + strand at 899.321 kb on - strand at 899.358 kb on + strand at 899.359 kb on - strand at 899.359 kb on - strand at 899.404 kb on - strand at 899.415 kb on + strand at 899.436 kb on + strand at 899.482 kb on - strand, within PS417_04005 at 899.490 kb on + strand, within PS417_04005 at 899.491 kb on - strand, within PS417_04005 at 899.628 kb on + strand, within PS417_04005 at 899.628 kb on + strand, within PS417_04005 at 899.628 kb on + strand at 899.659 kb on + strand, within PS417_04005 at 899.820 kb on + strand, within PS417_04005 at 899.820 kb on + strand, within PS417_04005 at 899.820 kb on + strand, within PS417_04005 at 899.821 kb on - strand, within PS417_04005 at 899.821 kb on - strand, within PS417_04005 at 899.821 kb on - strand, within PS417_04005 at 899.821 kb on - strand, within PS417_04005 at 899.821 kb on - strand, within PS417_04005 at 900.096 kb on + strand, within PS417_04005 at 900.096 kb on + strand, within PS417_04005 at 900.096 kb on + strand, within PS417_04005 at 900.096 kb on + strand, within PS417_04005 at 900.096 kb on + strand, within PS417_04005 at 900.096 kb on + strand, within PS417_04005 at 900.097 kb on - strand, within PS417_04005 at 900.097 kb on - strand, within PS417_04005 at 900.097 kb on - strand, within PS417_04005 at 900.097 kb on - strand, within PS417_04005 at 900.097 kb on - strand, within PS417_04005 at 900.187 kb on - strand, within PS417_04005 at 900.187 kb on - strand, within PS417_04005 at 900.187 kb on - strand, within PS417_04005 at 900.229 kb on + strand, within PS417_04005 at 900.230 kb on - strand, within PS417_04005 at 900.273 kb on + strand, within PS417_04005 at 900.274 kb on - strand, within PS417_04005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 897,639 - -0.6 897,698 + +0.3 898,075 + PS417_03995 0.31 -0.6 898,075 + PS417_03995 0.31 +1.1 898,075 + PS417_03995 0.31 -1.8 898,125 - PS417_03995 0.38 +0.2 898,125 - PS417_03995 0.38 +0.0 898,374 - PS417_03995 0.75 -0.9 898,511 + -0.2 898,512 - +0.7 898,512 - +0.1 898,559 + -0.3 898,560 - -1.0 898,788 + PS417_04000 0.32 -1.3 898,855 + PS417_04000 0.41 -0.4 898,894 + PS417_04000 0.46 +1.0 898,894 + PS417_04000 0.46 -0.9 898,895 - PS417_04000 0.46 +0.8 898,895 - PS417_04000 0.46 +1.0 898,895 - PS417_04000 0.46 -0.3 898,895 - PS417_04000 0.46 -1.0 898,895 - PS417_04000 0.46 -0.1 898,900 + PS417_04000 0.47 +1.3 898,900 + PS417_04000 0.47 +0.3 898,900 + PS417_04000 0.47 +0.7 898,901 - PS417_04000 0.47 +0.6 898,963 + PS417_04000 0.55 +0.7 899,117 - PS417_04000 0.75 -0.6 899,270 - +1.4 899,270 - -1.9 899,300 - -0.1 899,320 + +0.5 899,321 - -0.8 899,358 + -0.2 899,359 - -1.7 899,359 - +0.8 899,404 - -1.6 899,415 + -0.6 899,436 + +0.3 899,482 - PS417_04005 0.11 +1.7 899,490 + PS417_04005 0.12 -1.2 899,491 - PS417_04005 0.12 -0.9 899,628 + PS417_04005 0.24 +3.7 899,628 + PS417_04005 0.24 -0.1 899,628 + +0.7 899,659 + PS417_04005 0.26 -2.6 899,820 + PS417_04005 0.40 -0.5 899,820 + PS417_04005 0.40 -0.8 899,820 + PS417_04005 0.40 -1.0 899,821 - PS417_04005 0.40 -2.0 899,821 - PS417_04005 0.40 -0.7 899,821 - PS417_04005 0.40 -2.2 899,821 - PS417_04005 0.40 -0.6 899,821 - PS417_04005 0.40 -2.2 900,096 + PS417_04005 0.64 +0.1 900,096 + PS417_04005 0.64 -0.0 900,096 + PS417_04005 0.64 -0.7 900,096 + PS417_04005 0.64 -1.2 900,096 + PS417_04005 0.64 -1.1 900,096 + PS417_04005 0.64 -0.2 900,097 - PS417_04005 0.64 -0.1 900,097 - PS417_04005 0.64 -0.4 900,097 - PS417_04005 0.64 +0.3 900,097 - PS417_04005 0.64 -1.3 900,097 - PS417_04005 0.64 -1.4 900,187 - PS417_04005 0.72 -0.3 900,187 - PS417_04005 0.72 -0.1 900,187 - PS417_04005 0.72 -1.2 900,229 + PS417_04005 0.75 +0.1 900,230 - PS417_04005 0.76 +0.2 900,273 + PS417_04005 0.79 -0.8 900,274 - PS417_04005 0.79 -2.3
Or see this region's nucleotide sequence