Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03945 and PS417_03950 are separated by 126 nucleotides PS417_03950 and PS417_03955 overlap by 54 nucleotides PS417_03955 and PS417_03960 are separated by 2 nucleotides
PS417_03945: PS417_03945 - chemotaxis protein, at 880,925 to 882,955
_03945
PS417_03950: PS417_03950 - hypothetical protein, at 883,082 to 883,513
_03950
PS417_03955: PS417_03955 - hydrolase TatD, at 883,460 to 884,236
_03955
PS417_03960: PS417_03960 - transcriptional regulator, at 884,239 to 885,234
_03960
Position (kb) 883
884
885 Strain fitness (log2 ratio) -2
-1
0
1
2 at 882.679 kb on + strand, within PS417_03945 at 882.967 kb on - strand at 883.126 kb on + strand at 883.135 kb on + strand, within PS417_03950 at 883.136 kb on - strand, within PS417_03950 at 883.380 kb on + strand, within PS417_03950 at 883.380 kb on + strand, within PS417_03950 at 883.381 kb on - strand, within PS417_03950 at 883.381 kb on - strand, within PS417_03950 at 883.381 kb on - strand, within PS417_03950 at 883.381 kb on - strand, within PS417_03950 at 883.381 kb on - strand, within PS417_03950 at 883.461 kb on + strand at 883.590 kb on + strand, within PS417_03955 at 883.629 kb on - strand, within PS417_03955 at 883.732 kb on - strand, within PS417_03955 at 883.932 kb on + strand, within PS417_03955 at 883.932 kb on + strand, within PS417_03955 at 884.118 kb on + strand, within PS417_03955 at 884.118 kb on + strand, within PS417_03955 at 884.118 kb on + strand, within PS417_03955 at 884.119 kb on - strand, within PS417_03955 at 884.119 kb on - strand, within PS417_03955 at 884.253 kb on + strand at 884.253 kb on + strand at 885.179 kb on + strand at 885.179 kb on + strand at 885.179 kb on + strand at 885.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 882,679 + PS417_03945 0.86 +0.6 882,967 - +0.6 883,126 + -0.9 883,135 + PS417_03950 0.12 -0.4 883,136 - PS417_03950 0.12 +0.7 883,380 + PS417_03950 0.69 +0.5 883,380 + PS417_03950 0.69 -0.3 883,381 - PS417_03950 0.69 +0.3 883,381 - PS417_03950 0.69 +0.5 883,381 - PS417_03950 0.69 -1.1 883,381 - PS417_03950 0.69 -1.3 883,381 - PS417_03950 0.69 -1.5 883,461 + -2.3 883,590 + PS417_03955 0.17 +1.0 883,629 - PS417_03955 0.22 +2.0 883,732 - PS417_03955 0.35 -0.5 883,932 + PS417_03955 0.61 +0.9 883,932 + PS417_03955 0.61 +0.5 884,118 + PS417_03955 0.85 -1.0 884,118 + PS417_03955 0.85 +0.0 884,118 + PS417_03955 0.85 -0.6 884,119 - PS417_03955 0.85 -0.3 884,119 - PS417_03955 0.85 +1.7 884,253 + +0.6 884,253 + -1.1 885,179 + +0.2 885,179 + +0.5 885,179 + -0.5 885,180 - +2.1
Or see this region's nucleotide sequence