Strain Fitness in Pseudomonas simiae WCS417 around PS417_00365

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00360 and PS417_00365 are separated by 265 nucleotidesPS417_00365 and PS417_00370 are separated by 98 nucleotidesPS417_00370 and PS417_00375 are separated by 37 nucleotides PS417_00360: PS417_00360 - methionine ABC transporter ATP-binding protein, at 69,194 to 70,201 _00360 PS417_00365: PS417_00365 - hydroperoxidase, at 70,467 to 72,608 _00365 PS417_00370: PS417_00370 - hypothetical protein, at 72,707 to 73,426 _00370 PS417_00375: PS417_00375 - membrane protein, at 73,464 to 74,252 _00375 Position (kb) 70 71 72 73Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 70.178 kb on - strandat 70.204 kb on - strandat 70.204 kb on - strandat 70.260 kb on + strandat 70.372 kb on - strandat 70.372 kb on - strandat 70.375 kb on + strandat 70.375 kb on + strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.376 kb on - strandat 70.452 kb on - strandat 70.452 kb on - strandat 70.768 kb on - strand, within PS417_00365at 70.780 kb on - strand, within PS417_00365at 70.832 kb on + strand, within PS417_00365at 70.890 kb on - strand, within PS417_00365at 70.920 kb on - strand, within PS417_00365at 71.184 kb on - strand, within PS417_00365at 71.184 kb on - strand, within PS417_00365at 71.308 kb on - strand, within PS417_00365at 71.350 kb on - strand, within PS417_00365at 71.378 kb on + strand, within PS417_00365at 71.602 kb on - strand, within PS417_00365at 71.681 kb on + strand, within PS417_00365at 71.736 kb on + strand, within PS417_00365at 71.736 kb on + strand, within PS417_00365at 71.737 kb on - strandat 71.937 kb on + strand, within PS417_00365at 71.937 kb on + strand, within PS417_00365at 71.938 kb on - strand, within PS417_00365at 71.939 kb on + strand, within PS417_00365at 71.940 kb on - strand, within PS417_00365at 71.940 kb on - strand, within PS417_00365at 72.320 kb on + strand, within PS417_00365at 72.423 kb on + strandat 72.424 kb on - strandat 72.424 kb on - strandat 72.444 kb on - strandat 72.584 kb on + strandat 72.585 kb on - strandat 72.591 kb on - strandat 73.183 kb on - strand, within PS417_00370at 73.440 kb on + strandat 73.465 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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70,178 - +0.1
70,204 - -0.7
70,204 - +0.3
70,260 + -0.3
70,372 - -0.5
70,372 - -1.5
70,375 + -1.5
70,375 + +1.9
70,376 - +1.7
70,376 - +0.4
70,376 - +1.7
70,376 - -2.0
70,376 - +0.6
70,376 - +1.7
70,376 - -0.1
70,376 - +0.0
70,452 - +0.6
70,452 - -1.5
70,768 - PS417_00365 0.14 +1.0
70,780 - PS417_00365 0.15 -1.1
70,832 + PS417_00365 0.17 -0.4
70,890 - PS417_00365 0.20 -0.2
70,920 - PS417_00365 0.21 -0.9
71,184 - PS417_00365 0.33 +0.0
71,184 - PS417_00365 0.33 -1.9
71,308 - PS417_00365 0.39 -0.2
71,350 - PS417_00365 0.41 -0.0
71,378 + PS417_00365 0.43 +1.1
71,602 - PS417_00365 0.53 +0.4
71,681 + PS417_00365 0.57 +0.9
71,736 + PS417_00365 0.59 -2.1
71,736 + PS417_00365 0.59 +0.6
71,737 - +2.0
71,937 + PS417_00365 0.69 +0.5
71,937 + PS417_00365 0.69 -1.2
71,938 - PS417_00365 0.69 -0.0
71,939 + PS417_00365 0.69 -3.9
71,940 - PS417_00365 0.69 +0.3
71,940 - PS417_00365 0.69 +0.1
72,320 + PS417_00365 0.87 -0.7
72,423 + -0.7
72,424 - +1.1
72,424 - +2.3
72,444 - +0.4
72,584 + -1.8
72,585 - -2.0
72,591 - +0.8
73,183 - PS417_00370 0.66 -3.1
73,440 + +0.4
73,465 + -0.1

Or see this region's nucleotide sequence