Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03255 and PS417_03260 are separated by 139 nucleotides PS417_03260 and PS417_03265 are separated by 158 nucleotides PS417_03265 and PS417_03270 overlap by 14 nucleotides PS417_03270 and PS417_03275 overlap by 8 nucleotides
PS417_03255: PS417_03255 - 23S ribosomal RNA, at 744,427 to 747,342
_03255
PS417_03260: PS417_03260 - 5S ribosomal RNA, at 747,482 to 747,597
_03260
PS417_03265: PS417_03265 - paraquat-inducible protein A, at 747,756 to 748,415
_03265
PS417_03270: PS417_03270 - paraquat-inducible protein A, at 748,402 to 749,025
_03270
PS417_03275: PS417_03275 - mammalian cell entry protein, at 749,018 to 751,321
_03275
Position (kb)
747
748
749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 747.582 kb on + strand, within PS417_03260 at 747.582 kb on + strand, within PS417_03260 at 747.694 kb on + strand at 747.694 kb on + strand at 747.713 kb on + strand at 747.713 kb on + strand at 747.714 kb on - strand at 747.714 kb on - strand at 747.714 kb on - strand at 747.714 kb on - strand at 747.719 kb on - strand at 747.751 kb on + strand at 747.755 kb on + strand at 747.755 kb on + strand at 747.755 kb on + strand at 747.755 kb on + strand at 747.756 kb on - strand at 747.756 kb on - strand at 747.756 kb on - strand at 747.898 kb on + strand, within PS417_03265 at 747.899 kb on - strand, within PS417_03265 at 747.899 kb on - strand, within PS417_03265 at 747.899 kb on - strand, within PS417_03265 at 747.901 kb on - strand, within PS417_03265 at 747.963 kb on + strand, within PS417_03265 at 747.964 kb on - strand, within PS417_03265 at 748.003 kb on + strand, within PS417_03265 at 748.004 kb on - strand, within PS417_03265 at 748.004 kb on - strand, within PS417_03265 at 748.087 kb on + strand, within PS417_03265 at 748.176 kb on + strand, within PS417_03265 at 748.177 kb on - strand, within PS417_03265 at 748.200 kb on + strand, within PS417_03265 at 748.201 kb on - strand, within PS417_03265 at 748.201 kb on - strand, within PS417_03265 at 748.324 kb on + strand, within PS417_03265 at 748.711 kb on - strand, within PS417_03270 at 748.711 kb on - strand, within PS417_03270 at 748.728 kb on + strand, within PS417_03270 at 748.728 kb on + strand, within PS417_03270 at 748.729 kb on - strand, within PS417_03270 at 748.742 kb on + strand, within PS417_03270 at 748.743 kb on - strand, within PS417_03270 at 748.808 kb on - strand, within PS417_03270 at 748.996 kb on - strand at 749.038 kb on + strand at 749.213 kb on - strand at 749.399 kb on + strand, within PS417_03275 at 749.399 kb on + strand, within PS417_03275 at 749.400 kb on - strand, within PS417_03275 at 749.400 kb on - strand, within PS417_03275 at 749.400 kb on - strand, within PS417_03275 at 749.400 kb on - strand, within PS417_03275 at 749.400 kb on - strand, within PS417_03275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 747,582 + PS417_03260 0.86 +1.7 747,582 + PS417_03260 0.86 -3.7 747,694 + +0.1 747,694 + -1.0 747,713 + -0.9 747,713 + +0.4 747,714 - +1.1 747,714 - +1.1 747,714 - +0.3 747,714 - +0.2 747,719 - +1.5 747,751 + +0.6 747,755 + -0.9 747,755 + +1.0 747,755 + +1.0 747,755 + +0.5 747,756 - +1.4 747,756 - +1.0 747,756 - +1.0 747,898 + PS417_03265 0.22 +1.0 747,899 - PS417_03265 0.22 +1.7 747,899 - PS417_03265 0.22 +1.6 747,899 - PS417_03265 0.22 +0.6 747,901 - PS417_03265 0.22 +0.6 747,963 + PS417_03265 0.31 -0.1 747,964 - PS417_03265 0.32 +0.7 748,003 + PS417_03265 0.37 +0.9 748,004 - PS417_03265 0.38 +2.1 748,004 - PS417_03265 0.38 +2.5 748,087 + PS417_03265 0.50 -1.5 748,176 + PS417_03265 0.64 +1.5 748,177 - PS417_03265 0.64 +2.0 748,200 + PS417_03265 0.67 +0.6 748,201 - PS417_03265 0.67 +1.5 748,201 - PS417_03265 0.67 +1.1 748,324 + PS417_03265 0.86 +1.3 748,711 - PS417_03270 0.50 +1.4 748,711 - PS417_03270 0.50 +0.3 748,728 + PS417_03270 0.52 +1.8 748,728 + PS417_03270 0.52 +1.5 748,729 - PS417_03270 0.52 +1.3 748,742 + PS417_03270 0.54 +1.3 748,743 - PS417_03270 0.55 +0.4 748,808 - PS417_03270 0.65 -1.1 748,996 - +1.1 749,038 + +0.6 749,213 - +0.1 749,399 + PS417_03275 0.17 +1.4 749,399 + PS417_03275 0.17 +0.5 749,400 - PS417_03275 0.17 +1.5 749,400 - PS417_03275 0.17 +0.4 749,400 - PS417_03275 0.17 -2.7 749,400 - PS417_03275 0.17 +1.2 749,400 - PS417_03275 0.17 -0.3
Or see this region's nucleotide sequence