Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28010 and PS417_28015 are separated by 37 nucleotides PS417_28015 and PS417_28020 are separated by 196 nucleotides PS417_28020 and PS417_28025 overlap by 1 nucleotides PS417_28025 and PS417_28030 are separated by 111 nucleotides PS417_28030 and PS417_28035 are separated by 155 nucleotides
PS417_28010: PS417_28010 - pyridine nucleotide-disulfide oxidoreductase, at 6,046,340 to 6,047,488
_28010
PS417_28015: PS417_28015 - rubredoxin, at 6,047,526 to 6,047,693
_28015
PS417_28020: PS417_28020 - chorismate--pyruvate lyase, at 6,047,890 to 6,048,453
_28020
PS417_28025: PS417_28025 - 4-hydroxybenzoate polyprenyltransferase, at 6,048,453 to 6,049,343
_28025
PS417_28030: PS417_28030 - hypothetical protein, at 6,049,455 to 6,049,832
_28030
PS417_28035: PS417_28035 - transcriptional regulator PhoB, at 6,049,988 to 6,050,677
_28035
Position (kb)
6048
6049
6050 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6047.502 kb on + strand at 6047.503 kb on - strand at 6047.505 kb on + strand at 6047.505 kb on + strand at 6047.505 kb on + strand at 6047.505 kb on + strand at 6047.505 kb on + strand at 6047.506 kb on - strand at 6047.506 kb on - strand at 6047.506 kb on - strand at 6047.506 kb on - strand at 6047.509 kb on + strand at 6047.509 kb on + strand at 6047.509 kb on + strand at 6047.510 kb on - strand at 6047.656 kb on + strand, within PS417_28015 at 6047.656 kb on + strand, within PS417_28015 at 6047.657 kb on - strand, within PS417_28015 at 6047.657 kb on - strand, within PS417_28015 at 6047.657 kb on - strand, within PS417_28015 at 6047.673 kb on + strand, within PS417_28015 at 6047.673 kb on + strand, within PS417_28015 at 6047.694 kb on + strand at 6047.694 kb on + strand at 6047.695 kb on - strand at 6047.699 kb on - strand at 6047.851 kb on + strand at 6047.852 kb on - strand at 6048.136 kb on + strand, within PS417_28020 at 6048.136 kb on + strand, within PS417_28020 at 6048.136 kb on + strand at 6048.137 kb on - strand, within PS417_28020 at 6048.216 kb on + strand, within PS417_28020 at 6048.216 kb on + strand, within PS417_28020 at 6048.456 kb on + strand at 6049.529 kb on - strand, within PS417_28030 at 6049.534 kb on + strand, within PS417_28030 at 6049.534 kb on + strand, within PS417_28030 at 6049.535 kb on - strand, within PS417_28030 at 6049.535 kb on - strand, within PS417_28030 at 6049.535 kb on - strand, within PS417_28030 at 6049.552 kb on + strand, within PS417_28030 at 6049.611 kb on - strand, within PS417_28030 at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.825 kb on + strand at 6049.826 kb on - strand at 6049.826 kb on - strand at 6049.826 kb on - strand at 6049.877 kb on - strand at 6049.893 kb on - strand at 6049.933 kb on - strand at 6049.945 kb on + strand at 6049.946 kb on - strand at 6050.069 kb on + strand, within PS417_28035 at 6050.294 kb on + strand, within PS417_28035 at 6050.294 kb on + strand, within PS417_28035 at 6050.295 kb on - strand, within PS417_28035 at 6050.295 kb on - strand, within PS417_28035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 6,047,502 + +1.1 6,047,503 - -1.2 6,047,505 + -2.3 6,047,505 + -0.7 6,047,505 + -1.2 6,047,505 + +0.7 6,047,505 + -0.2 6,047,506 - -1.1 6,047,506 - -0.6 6,047,506 - -1.1 6,047,506 - -1.0 6,047,509 + +0.6 6,047,509 + -1.1 6,047,509 + -0.3 6,047,510 - -1.3 6,047,656 + PS417_28015 0.77 -0.3 6,047,656 + PS417_28015 0.77 +0.2 6,047,657 - PS417_28015 0.78 -2.0 6,047,657 - PS417_28015 0.78 -1.4 6,047,657 - PS417_28015 0.78 +1.1 6,047,673 + PS417_28015 0.88 +0.2 6,047,673 + PS417_28015 0.88 +0.2 6,047,694 + -0.3 6,047,694 + -0.8 6,047,695 - +0.0 6,047,699 - -1.6 6,047,851 + -0.5 6,047,852 - +2.3 6,048,136 + PS417_28020 0.44 -0.9 6,048,136 + PS417_28020 0.44 +1.2 6,048,136 + +0.7 6,048,137 - PS417_28020 0.44 +0.5 6,048,216 + PS417_28020 0.58 +3.2 6,048,216 + PS417_28020 0.58 +0.3 6,048,456 + -0.5 6,049,529 - PS417_28030 0.20 +1.9 6,049,534 + PS417_28030 0.21 -0.8 6,049,534 + PS417_28030 0.21 +0.3 6,049,535 - PS417_28030 0.21 +1.0 6,049,535 - PS417_28030 0.21 -0.0 6,049,535 - PS417_28030 0.21 -1.3 6,049,552 + PS417_28030 0.26 +2.5 6,049,611 - PS417_28030 0.41 -0.2 6,049,825 + +1.5 6,049,825 + +2.0 6,049,825 + +2.2 6,049,825 + +1.4 6,049,825 + +0.2 6,049,826 - +0.7 6,049,826 - -1.1 6,049,826 - +1.2 6,049,877 - -0.1 6,049,893 - -0.5 6,049,933 - +1.0 6,049,945 + +1.8 6,049,946 - +0.4 6,050,069 + PS417_28035 0.12 +0.4 6,050,294 + PS417_28035 0.44 +1.1 6,050,294 + PS417_28035 0.44 -0.2 6,050,295 - PS417_28035 0.44 +0.2 6,050,295 - PS417_28035 0.44 +0.8
Or see this region's nucleotide sequence