Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25660 and PS417_25665 are separated by 136 nucleotides PS417_25665 and PS417_25670 are separated by 24 nucleotides PS417_25670 and PS417_25675 are separated by 70 nucleotides
PS417_25660: PS417_25660 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,542,898 to 5,543,932
_25660
PS417_25665: PS417_25665 - membrane protein, at 5,544,069 to 5,544,497
_25665
PS417_25670: PS417_25670 - 2-nitropropane dioxygenase, at 5,544,522 to 5,545,484
_25670
PS417_25675: PS417_25675 - membrane protein, at 5,545,555 to 5,546,511
_25675
Position (kb)
5544
5545
5546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5543.796 kb on + strand, within PS417_25660 at 5543.933 kb on - strand at 5543.973 kb on + strand at 5543.973 kb on + strand at 5544.012 kb on + strand at 5544.013 kb on - strand at 5544.031 kb on - strand at 5544.033 kb on + strand at 5544.033 kb on + strand at 5544.033 kb on + strand at 5544.033 kb on + strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.034 kb on - strand at 5544.064 kb on + strand at 5544.496 kb on - strand at 5544.496 kb on - strand at 5544.505 kb on + strand at 5544.825 kb on - strand, within PS417_25670 at 5544.853 kb on + strand, within PS417_25670 at 5544.854 kb on - strand, within PS417_25670 at 5544.862 kb on + strand, within PS417_25670 at 5545.111 kb on - strand, within PS417_25670 at 5545.161 kb on + strand, within PS417_25670 at 5545.357 kb on + strand, within PS417_25670 at 5545.358 kb on - strand, within PS417_25670 at 5545.426 kb on + strand at 5545.426 kb on + strand at 5545.426 kb on + strand at 5545.426 kb on + strand at 5545.522 kb on + strand at 5545.522 kb on + strand at 5545.736 kb on - strand, within PS417_25675 at 5545.904 kb on - strand, within PS417_25675 at 5546.069 kb on + strand, within PS417_25675 at 5546.115 kb on - strand, within PS417_25675 at 5546.164 kb on + strand, within PS417_25675 at 5546.248 kb on + strand, within PS417_25675 at 5546.383 kb on - strand, within PS417_25675 at 5546.395 kb on + strand, within PS417_25675 at 5546.395 kb on + strand, within PS417_25675 at 5546.395 kb on + strand, within PS417_25675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,543,796 + PS417_25660 0.87 -0.8 5,543,933 - -1.4 5,543,973 + +0.0 5,543,973 + -0.8 5,544,012 + -0.7 5,544,013 - +0.3 5,544,031 - +2.3 5,544,033 + +0.7 5,544,033 + +0.1 5,544,033 + -0.4 5,544,033 + -3.4 5,544,034 - -1.0 5,544,034 - +0.2 5,544,034 - -1.3 5,544,034 - -0.8 5,544,034 - +1.0 5,544,064 + -0.9 5,544,496 - -0.4 5,544,496 - +0.2 5,544,505 + +0.9 5,544,825 - PS417_25670 0.31 -0.4 5,544,853 + PS417_25670 0.34 -0.9 5,544,854 - PS417_25670 0.34 -0.5 5,544,862 + PS417_25670 0.35 -1.7 5,545,111 - PS417_25670 0.61 -2.4 5,545,161 + PS417_25670 0.66 +0.8 5,545,357 + PS417_25670 0.87 -0.8 5,545,358 - PS417_25670 0.87 -1.1 5,545,426 + +2.0 5,545,426 + -0.2 5,545,426 + -1.0 5,545,426 + -0.9 5,545,522 + +0.5 5,545,522 + +0.5 5,545,736 - PS417_25675 0.19 +0.5 5,545,904 - PS417_25675 0.36 -1.5 5,546,069 + PS417_25675 0.54 -0.4 5,546,115 - PS417_25675 0.59 +0.7 5,546,164 + PS417_25675 0.64 +0.1 5,546,248 + PS417_25675 0.72 +0.7 5,546,383 - PS417_25675 0.87 +0.4 5,546,395 + PS417_25675 0.88 +0.7 5,546,395 + PS417_25675 0.88 -0.6 5,546,395 + PS417_25675 0.88 +1.0
Or see this region's nucleotide sequence