Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24005 and PS417_24010 are separated by 16 nucleotides PS417_24010 and PS417_24015 are separated by 8 nucleotides PS417_24015 and PS417_24020 are separated by 200 nucleotides PS417_24020 and PS417_24025 are separated by 106 nucleotides
PS417_24005: PS417_24005 - phosphoglucosamine mutase, at 5,196,631 to 5,197,968
_24005
PS417_24010: PS417_24010 - dihydropteroate synthase, at 5,197,985 to 5,198,836
_24010
PS417_24015: PS417_24015 - ATP-dependent metalloprotease, at 5,198,845 to 5,200,755
_24015
PS417_24020: PS417_24020 - 23S rRNA methyltransferase, at 5,200,956 to 5,201,606
_24020
PS417_24025: PS417_24025 - RNA-binding protein, at 5,201,713 to 5,202,021
_24025
Position (kb) 5198
5199
5200
5201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5197.969 kb on - strand at 5197.969 kb on - strand at 5197.969 kb on - strand at 5197.969 kb on - strand at 5198.847 kb on - strand at 5198.847 kb on - strand at 5200.832 kb on - strand at 5200.835 kb on - strand at 5200.844 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,197,969 - -1.1 5,197,969 - +3.3 5,197,969 - -0.5 5,197,969 - +3.0 5,198,847 - +0.1 5,198,847 - -1.1 5,200,832 - -1.5 5,200,835 - -1.4 5,200,844 - -2.9
Or see this region's nucleotide sequence