Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23830 and PS417_23835 are separated by 93 nucleotides PS417_23835 and PS417_23840 are separated by 192 nucleotides PS417_23840 and PS417_23845 are separated by 251 nucleotides
PS417_23830: PS417_23830 - hypothetical protein, at 5,157,542 to 5,159,098
_23830
PS417_23835: PS417_23835 - hypothetical protein, at 5,159,192 to 5,159,536
_23835
PS417_23840: PS417_23840 - competence protein TfoX, at 5,159,729 to 5,160,001
_23840
PS417_23845: PS417_23845 - hemin ABC transporter ATP-binding protein, at 5,160,253 to 5,161,020
_23845
Position (kb) 5159
5160
5161 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5158.733 kb on + strand, within PS417_23830 at 5158.798 kb on + strand, within PS417_23830 at 5158.799 kb on - strand, within PS417_23830 at 5158.834 kb on + strand, within PS417_23830 at 5158.839 kb on + strand, within PS417_23830 at 5158.839 kb on + strand, within PS417_23830 at 5158.839 kb on + strand, within PS417_23830 at 5158.840 kb on - strand, within PS417_23830 at 5159.071 kb on + strand at 5159.072 kb on - strand at 5159.147 kb on + strand at 5159.157 kb on - strand at 5159.158 kb on + strand at 5159.169 kb on + strand at 5159.338 kb on + strand, within PS417_23835 at 5159.366 kb on + strand, within PS417_23835 at 5159.456 kb on + strand, within PS417_23835 at 5159.456 kb on + strand, within PS417_23835 at 5159.456 kb on + strand, within PS417_23835 at 5159.457 kb on - strand, within PS417_23835 at 5159.610 kb on + strand at 5159.662 kb on - strand at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5160.025 kb on - strand at 5160.169 kb on + strand at 5160.169 kb on + strand at 5160.170 kb on - strand at 5160.170 kb on - strand at 5160.226 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.232 kb on + strand at 5160.319 kb on + strand at 5160.319 kb on + strand at 5160.320 kb on - strand at 5160.320 kb on - strand at 5160.320 kb on - strand at 5160.360 kb on + strand, within PS417_23845 at 5160.630 kb on - strand, within PS417_23845 at 5160.897 kb on + strand, within PS417_23845 at 5160.898 kb on - strand, within PS417_23845 at 5160.898 kb on - strand, within PS417_23845 at 5160.898 kb on - strand, within PS417_23845 at 5160.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,158,733 + PS417_23830 0.76 -0.6 5,158,798 + PS417_23830 0.81 -0.6 5,158,799 - PS417_23830 0.81 -0.3 5,158,834 + PS417_23830 0.83 +0.9 5,158,839 + PS417_23830 0.83 +0.7 5,158,839 + PS417_23830 0.83 -0.1 5,158,839 + PS417_23830 0.83 +0.0 5,158,840 - PS417_23830 0.83 +0.4 5,159,071 + -0.5 5,159,072 - +1.5 5,159,147 + +1.1 5,159,157 - +0.1 5,159,158 + -0.7 5,159,169 + +1.3 5,159,338 + PS417_23835 0.42 -0.4 5,159,366 + PS417_23835 0.50 -0.4 5,159,456 + PS417_23835 0.77 +1.2 5,159,456 + PS417_23835 0.77 -0.3 5,159,456 + PS417_23835 0.77 -1.2 5,159,457 - PS417_23835 0.77 +0.0 5,159,610 + -0.2 5,159,662 - +0.6 5,159,891 + PS417_23840 0.59 +2.7 5,159,891 + PS417_23840 0.59 -0.1 5,159,891 + PS417_23840 0.59 -0.1 5,159,891 + PS417_23840 0.59 +2.4 5,159,891 + PS417_23840 0.59 -0.4 5,159,892 - PS417_23840 0.60 +0.1 5,159,892 - PS417_23840 0.60 +0.4 5,159,892 - PS417_23840 0.60 -0.1 5,159,892 - PS417_23840 0.60 +1.7 5,160,025 - -1.9 5,160,169 + +0.3 5,160,169 + -1.3 5,160,170 - -0.8 5,160,170 - -4.8 5,160,226 + +0.2 5,160,230 + -0.6 5,160,230 + -0.4 5,160,230 + -0.4 5,160,230 + -0.5 5,160,230 + +0.1 5,160,230 + -1.5 5,160,232 + -0.0 5,160,319 + +0.9 5,160,319 + -1.2 5,160,320 - +0.1 5,160,320 - -1.5 5,160,320 - +0.2 5,160,360 + PS417_23845 0.14 +1.7 5,160,630 - PS417_23845 0.49 -0.2 5,160,897 + PS417_23845 0.84 +0.3 5,160,898 - PS417_23845 0.84 +0.7 5,160,898 - PS417_23845 0.84 -0.5 5,160,898 - PS417_23845 0.84 +1.7 5,160,973 + -1.1
Or see this region's nucleotide sequence