Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22245 and PS417_22250 are separated by 196 nucleotides PS417_22250 and PS417_22255 are separated by 144 nucleotides PS417_22255 and PS417_22260 are separated by 56 nucleotides
PS417_22245: PS417_22245 - leucyl aminopeptidase (aminopeptidase T), at 4,821,193 to 4,822,152
_22245
PS417_22250: PS417_22250 - pseudouridine synthase, at 4,822,349 to 4,823,560
_22250
PS417_22255: PS417_22255 - Fe-S oxidoreductase, at 4,823,705 to 4,823,896
_22255
PS417_22260: PS417_22260 - segregation and condensation protein B, at 4,823,953 to 4,824,891
_22260
Position (kb)
4822
4823
4824 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4821.441 kb on + strand, within PS417_22245 at 4821.442 kb on - strand, within PS417_22245 at 4821.539 kb on - strand, within PS417_22245 at 4821.547 kb on + strand, within PS417_22245 at 4821.553 kb on + strand, within PS417_22245 at 4821.553 kb on + strand, within PS417_22245 at 4821.554 kb on - strand, within PS417_22245 at 4821.554 kb on - strand, within PS417_22245 at 4821.823 kb on + strand, within PS417_22245 at 4821.824 kb on - strand, within PS417_22245 at 4821.922 kb on + strand, within PS417_22245 at 4821.923 kb on - strand, within PS417_22245 at 4821.997 kb on + strand, within PS417_22245 at 4821.998 kb on - strand, within PS417_22245 at 4821.998 kb on - strand, within PS417_22245 at 4822.151 kb on - strand at 4822.151 kb on - strand at 4822.181 kb on - strand at 4822.223 kb on + strand at 4822.528 kb on + strand, within PS417_22250 at 4822.636 kb on + strand, within PS417_22250 at 4822.637 kb on - strand, within PS417_22250 at 4823.631 kb on - strand at 4823.656 kb on - strand at 4823.897 kb on - strand at 4823.919 kb on - strand at 4823.919 kb on - strand at 4824.139 kb on + strand, within PS417_22260 at 4824.140 kb on - strand, within PS417_22260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,821,441 + PS417_22245 0.26 +0.5 4,821,442 - PS417_22245 0.26 -0.6 4,821,539 - PS417_22245 0.36 +0.3 4,821,547 + PS417_22245 0.37 +0.6 4,821,553 + PS417_22245 0.38 -0.3 4,821,553 + PS417_22245 0.38 -0.0 4,821,554 - PS417_22245 0.38 -2.0 4,821,554 - PS417_22245 0.38 -0.5 4,821,823 + PS417_22245 0.66 +1.1 4,821,824 - PS417_22245 0.66 +0.1 4,821,922 + PS417_22245 0.76 -1.3 4,821,923 - PS417_22245 0.76 -1.3 4,821,997 + PS417_22245 0.84 -0.4 4,821,998 - PS417_22245 0.84 -3.0 4,821,998 - PS417_22245 0.84 +1.6 4,822,151 - +0.7 4,822,151 - +0.1 4,822,181 - +0.4 4,822,223 + -0.7 4,822,528 + PS417_22250 0.15 -1.0 4,822,636 + PS417_22250 0.24 -0.3 4,822,637 - PS417_22250 0.24 -1.6 4,823,631 - -1.5 4,823,656 - +0.6 4,823,897 - -0.9 4,823,919 - -1.7 4,823,919 - +0.6 4,824,139 + PS417_22260 0.20 -0.7 4,824,140 - PS417_22260 0.20 -0.0
Or see this region's nucleotide sequence