Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21750 and PS417_21755 are separated by 396 nucleotides PS417_21755 and PS417_21760 are separated by 8 nucleotides PS417_21760 and PS417_21765 are separated by 104 nucleotides
PS417_21750: PS417_21750 - acetyl-CoA synthetase, at 4,723,071 to 4,725,026
_21750
PS417_21755: PS417_21755 - hypothetical protein, at 4,725,423 to 4,725,692
_21755
PS417_21760: PS417_21760 - hypothetical protein, at 4,725,701 to 4,726,072
_21760
PS417_21765: PS417_21765 - ribonucleotide-diphosphate reductase subunit beta, at 4,726,177 to 4,727,427
_21765
Position (kb)
4725
4726
4727 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4724.945 kb on - strand at 4724.956 kb on + strand at 4724.957 kb on - strand at 4724.988 kb on + strand at 4725.007 kb on + strand at 4725.034 kb on - strand at 4725.046 kb on + strand at 4725.046 kb on + strand at 4725.046 kb on + strand at 4725.046 kb on + strand at 4725.047 kb on - strand at 4725.047 kb on - strand at 4725.047 kb on - strand at 4725.058 kb on + strand at 4725.058 kb on + strand at 4725.059 kb on - strand at 4725.059 kb on - strand at 4725.086 kb on + strand at 4725.088 kb on + strand at 4725.107 kb on + strand at 4725.107 kb on + strand at 4725.107 kb on + strand at 4725.250 kb on - strand at 4725.258 kb on - strand at 4725.258 kb on - strand at 4725.357 kb on - strand at 4725.412 kb on + strand at 4725.412 kb on + strand at 4725.412 kb on + strand at 4725.413 kb on - strand at 4725.413 kb on - strand at 4725.413 kb on - strand at 4725.413 kb on - strand at 4725.415 kb on - strand at 4725.415 kb on - strand at 4725.415 kb on - strand at 4725.417 kb on - strand at 4725.417 kb on - strand at 4725.417 kb on - strand at 4725.433 kb on + strand at 4725.433 kb on + strand at 4725.436 kb on + strand at 4725.500 kb on - strand, within PS417_21755 at 4725.500 kb on - strand, within PS417_21755 at 4725.531 kb on + strand, within PS417_21755 at 4725.531 kb on + strand, within PS417_21755 at 4725.532 kb on - strand, within PS417_21755 at 4725.532 kb on - strand, within PS417_21755 at 4725.537 kb on + strand, within PS417_21755 at 4725.537 kb on + strand at 4725.537 kb on + strand, within PS417_21755 at 4725.622 kb on - strand, within PS417_21755 at 4725.653 kb on + strand, within PS417_21755 at 4725.654 kb on - strand, within PS417_21755 at 4725.658 kb on - strand, within PS417_21755 at 4725.695 kb on - strand at 4725.703 kb on - strand at 4725.916 kb on - strand, within PS417_21760 at 4725.916 kb on - strand, within PS417_21760 at 4726.065 kb on - strand at 4726.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,724,945 - -1.3 4,724,956 + -0.2 4,724,957 - -0.6 4,724,988 + -1.7 4,725,007 + +1.1 4,725,034 - +1.9 4,725,046 + +0.2 4,725,046 + -1.7 4,725,046 + +1.4 4,725,046 + +0.2 4,725,047 - +0.2 4,725,047 - +1.7 4,725,047 - -0.2 4,725,058 + +0.6 4,725,058 + -1.1 4,725,059 - +1.2 4,725,059 - -0.5 4,725,086 + -0.2 4,725,088 + +2.9 4,725,107 + +0.7 4,725,107 + +0.9 4,725,107 + -0.3 4,725,250 - -1.6 4,725,258 - -1.4 4,725,258 - -2.2 4,725,357 - +1.2 4,725,412 + -1.2 4,725,412 + +0.3 4,725,412 + +0.3 4,725,413 - +2.2 4,725,413 - -2.0 4,725,413 - +0.2 4,725,413 - -2.4 4,725,415 - -0.7 4,725,415 - -0.5 4,725,415 - -0.6 4,725,417 - +0.8 4,725,417 - -0.4 4,725,417 - -1.2 4,725,433 + +0.6 4,725,433 + +0.1 4,725,436 + -0.1 4,725,500 - PS417_21755 0.29 -1.3 4,725,500 - PS417_21755 0.29 +0.2 4,725,531 + PS417_21755 0.40 +0.3 4,725,531 + PS417_21755 0.40 +0.5 4,725,532 - PS417_21755 0.40 -1.8 4,725,532 - PS417_21755 0.40 -0.6 4,725,537 + PS417_21755 0.42 -0.6 4,725,537 + -1.7 4,725,537 + PS417_21755 0.42 +1.1 4,725,622 - PS417_21755 0.74 -0.2 4,725,653 + PS417_21755 0.85 -0.1 4,725,654 - PS417_21755 0.86 +0.1 4,725,658 - PS417_21755 0.87 -1.9 4,725,695 - -0.5 4,725,703 - -0.5 4,725,916 - PS417_21760 0.58 -1.7 4,725,916 - PS417_21760 0.58 -0.5 4,726,065 - +1.1 4,726,221 - +2.2
Or see this region's nucleotide sequence