Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21275 and PS417_21280 are separated by 270 nucleotides PS417_21280 and PS417_21285 overlap by 4 nucleotides PS417_21285 and PS417_21290 are separated by 9 nucleotides
PS417_21275: PS417_21275 - GntR family transcriptional regulator, at 4,624,011 to 4,625,450
_21275
PS417_21280: PS417_21280 - membrane protein, at 4,625,721 to 4,626,536
_21280
PS417_21285: PS417_21285 - hypothetical protein, at 4,626,533 to 4,626,895
_21285
PS417_21290: PS417_21290 - ABC transporter substrate-binding protein, at 4,626,905 to 4,627,720
_21290
Position (kb)
4625
4626
4627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4624.837 kb on + strand, within PS417_21275 at 4624.837 kb on + strand, within PS417_21275 at 4624.837 kb on + strand, within PS417_21275 at 4624.838 kb on - strand, within PS417_21275 at 4624.838 kb on - strand, within PS417_21275 at 4625.018 kb on - strand, within PS417_21275 at 4625.018 kb on - strand, within PS417_21275 at 4625.018 kb on - strand, within PS417_21275 at 4625.018 kb on - strand, within PS417_21275 at 4625.086 kb on - strand, within PS417_21275 at 4625.089 kb on + strand, within PS417_21275 at 4625.248 kb on + strand, within PS417_21275 at 4625.248 kb on + strand, within PS417_21275 at 4625.249 kb on - strand, within PS417_21275 at 4625.384 kb on - strand at 4625.384 kb on - strand at 4625.424 kb on - strand at 4625.424 kb on - strand at 4625.432 kb on - strand at 4625.510 kb on - strand at 4625.517 kb on + strand at 4625.599 kb on + strand at 4625.599 kb on + strand at 4625.600 kb on - strand at 4625.669 kb on - strand at 4625.670 kb on + strand at 4625.671 kb on - strand at 4625.709 kb on + strand at 4625.709 kb on + strand at 4625.710 kb on - strand at 4625.710 kb on - strand at 4625.833 kb on + strand, within PS417_21280 at 4625.834 kb on - strand, within PS417_21280 at 4625.880 kb on + strand, within PS417_21280 at 4625.881 kb on - strand, within PS417_21280 at 4625.881 kb on - strand, within PS417_21280 at 4625.943 kb on - strand, within PS417_21280 at 4626.272 kb on - strand, within PS417_21280 at 4626.286 kb on + strand, within PS417_21280 at 4626.292 kb on + strand at 4626.293 kb on - strand, within PS417_21280 at 4626.323 kb on - strand, within PS417_21280 at 4626.590 kb on + strand, within PS417_21285 at 4626.590 kb on + strand, within PS417_21285 at 4626.591 kb on - strand, within PS417_21285 at 4626.591 kb on - strand, within PS417_21285 at 4627.075 kb on - strand, within PS417_21290 at 4627.097 kb on + strand, within PS417_21290 at 4627.098 kb on - strand, within PS417_21290 at 4627.098 kb on - strand, within PS417_21290 at 4627.176 kb on + strand, within PS417_21290 at 4627.176 kb on + strand, within PS417_21290 at 4627.176 kb on + strand, within PS417_21290 at 4627.335 kb on + strand, within PS417_21290 at 4627.371 kb on + strand, within PS417_21290 at 4627.401 kb on + strand, within PS417_21290 at 4627.402 kb on - strand, within PS417_21290 at 4627.402 kb on - strand, within PS417_21290 at 4627.402 kb on - strand, within PS417_21290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,624,837 + PS417_21275 0.57 -1.2 4,624,837 + PS417_21275 0.57 -3.1 4,624,837 + PS417_21275 0.57 -1.1 4,624,838 - PS417_21275 0.57 +1.9 4,624,838 - PS417_21275 0.57 -1.1 4,625,018 - PS417_21275 0.70 -0.5 4,625,018 - PS417_21275 0.70 -1.3 4,625,018 - PS417_21275 0.70 +0.6 4,625,018 - PS417_21275 0.70 -0.8 4,625,086 - PS417_21275 0.75 -1.3 4,625,089 + PS417_21275 0.75 -1.5 4,625,248 + PS417_21275 0.86 -1.5 4,625,248 + PS417_21275 0.86 -1.0 4,625,249 - PS417_21275 0.86 -0.5 4,625,384 - -0.4 4,625,384 - +1.7 4,625,424 - -1.7 4,625,424 - -2.5 4,625,432 - -0.8 4,625,510 - +0.9 4,625,517 + -0.6 4,625,599 + -0.3 4,625,599 + -0.6 4,625,600 - +0.5 4,625,669 - -0.1 4,625,670 + -2.4 4,625,671 - -0.7 4,625,709 + +1.3 4,625,709 + +0.1 4,625,710 - -0.1 4,625,710 - -0.8 4,625,833 + PS417_21280 0.14 -0.6 4,625,834 - PS417_21280 0.14 +0.1 4,625,880 + PS417_21280 0.19 +1.3 4,625,881 - PS417_21280 0.20 +2.2 4,625,881 - PS417_21280 0.20 +0.2 4,625,943 - PS417_21280 0.27 +0.5 4,626,272 - PS417_21280 0.68 +0.1 4,626,286 + PS417_21280 0.69 -0.0 4,626,292 + +0.7 4,626,293 - PS417_21280 0.70 -0.5 4,626,323 - PS417_21280 0.74 +0.5 4,626,590 + PS417_21285 0.16 -0.1 4,626,590 + PS417_21285 0.16 +0.6 4,626,591 - PS417_21285 0.16 +0.3 4,626,591 - PS417_21285 0.16 +0.5 4,627,075 - PS417_21290 0.21 -2.3 4,627,097 + PS417_21290 0.24 +0.2 4,627,098 - PS417_21290 0.24 -0.4 4,627,098 - PS417_21290 0.24 -0.3 4,627,176 + PS417_21290 0.33 -0.1 4,627,176 + PS417_21290 0.33 +1.2 4,627,176 + PS417_21290 0.33 -0.4 4,627,335 + PS417_21290 0.53 -1.3 4,627,371 + PS417_21290 0.57 -0.5 4,627,401 + PS417_21290 0.61 +0.7 4,627,402 - PS417_21290 0.61 -0.3 4,627,402 - PS417_21290 0.61 +0.0 4,627,402 - PS417_21290 0.61 +0.6
Or see this region's nucleotide sequence