Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19900 and PS417_19905 are separated by 97 nucleotides PS417_19905 and PS417_19910 overlap by 1 nucleotides PS417_19910 and PS417_19915 are separated by 64 nucleotides
PS417_19900: PS417_19900 - phenylalanine 4-monooxygenase, at 4,322,200 to 4,322,991
_19900
PS417_19905: PS417_19905 - pterin-4-alpha-carbinolamine dehydratase, at 4,323,089 to 4,323,445
_19905
PS417_19910: PS417_19910 - aromatic amino acid aminotransferase, at 4,323,445 to 4,324,638
_19910
PS417_19915: PS417_19915 - serine/threonine protein phosphatase, at 4,324,703 to 4,325,512
_19915
Position (kb)
4323
4324
4325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4322.536 kb on + strand, within PS417_19900 at 4322.623 kb on + strand, within PS417_19900 at 4322.666 kb on - strand, within PS417_19900 at 4322.735 kb on - strand, within PS417_19900 at 4322.773 kb on + strand, within PS417_19900 at 4322.774 kb on - strand, within PS417_19900 at 4322.774 kb on - strand, within PS417_19900 at 4322.774 kb on - strand, within PS417_19900 at 4322.774 kb on - strand, within PS417_19900 at 4322.996 kb on + strand at 4322.997 kb on - strand at 4322.997 kb on - strand at 4323.444 kb on - strand at 4323.553 kb on + strand at 4323.553 kb on + strand at 4323.554 kb on - strand at 4323.556 kb on - strand at 4323.640 kb on + strand, within PS417_19910 at 4323.641 kb on - strand, within PS417_19910 at 4323.641 kb on - strand, within PS417_19910 at 4323.853 kb on - strand, within PS417_19910 at 4324.195 kb on + strand, within PS417_19910 at 4324.256 kb on + strand, within PS417_19910 at 4324.256 kb on + strand at 4324.257 kb on - strand, within PS417_19910 at 4324.492 kb on + strand, within PS417_19910 at 4324.546 kb on + strand at 4324.547 kb on - strand at 4324.547 kb on - strand at 4324.547 kb on - strand at 4324.547 kb on - strand at 4324.547 kb on - strand at 4324.547 kb on - strand at 4324.547 kb on - strand at 4324.548 kb on + strand at 4324.548 kb on + strand at 4324.765 kb on - strand at 4324.836 kb on + strand, within PS417_19915 at 4324.837 kb on - strand, within PS417_19915 at 4324.984 kb on - strand, within PS417_19915 at 4325.001 kb on + strand, within PS417_19915 at 4325.002 kb on - strand, within PS417_19915 at 4325.007 kb on + strand, within PS417_19915 at 4325.064 kb on + strand, within PS417_19915 at 4325.250 kb on + strand, within PS417_19915 at 4325.250 kb on + strand, within PS417_19915 at 4325.250 kb on + strand, within PS417_19915 at 4325.250 kb on + strand, within PS417_19915 at 4325.250 kb on + strand, within PS417_19915 at 4325.250 kb on + strand, within PS417_19915 at 4325.251 kb on - strand, within PS417_19915 at 4325.251 kb on - strand, within PS417_19915 at 4325.322 kb on + strand, within PS417_19915 at 4325.322 kb on + strand, within PS417_19915 at 4325.322 kb on + strand, within PS417_19915 at 4325.322 kb on + strand at 4325.323 kb on - strand, within PS417_19915 at 4325.323 kb on - strand, within PS417_19915 at 4325.560 kb on + strand at 4325.561 kb on - strand at 4325.565 kb on + strand at 4325.565 kb on + strand at 4325.619 kb on - strand at 4325.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,322,536 + PS417_19900 0.42 -1.7 4,322,623 + PS417_19900 0.53 +1.3 4,322,666 - PS417_19900 0.59 +0.5 4,322,735 - PS417_19900 0.68 +0.5 4,322,773 + PS417_19900 0.72 -2.6 4,322,774 - PS417_19900 0.72 -0.7 4,322,774 - PS417_19900 0.72 +1.3 4,322,774 - PS417_19900 0.72 +0.1 4,322,774 - PS417_19900 0.72 -0.2 4,322,996 + -1.1 4,322,997 - -0.2 4,322,997 - -0.7 4,323,444 - +0.1 4,323,553 + +0.4 4,323,553 + -0.1 4,323,554 - -0.2 4,323,556 - -0.2 4,323,640 + PS417_19910 0.16 -1.4 4,323,641 - PS417_19910 0.16 +0.3 4,323,641 - PS417_19910 0.16 -1.0 4,323,853 - PS417_19910 0.34 -2.4 4,324,195 + PS417_19910 0.63 -0.0 4,324,256 + PS417_19910 0.68 -0.2 4,324,256 + -1.0 4,324,257 - PS417_19910 0.68 -0.7 4,324,492 + PS417_19910 0.88 -1.6 4,324,546 + +0.2 4,324,547 - -2.1 4,324,547 - +0.0 4,324,547 - +0.7 4,324,547 - +1.4 4,324,547 - -0.1 4,324,547 - -0.1 4,324,547 - -0.2 4,324,548 + -0.5 4,324,548 + -1.4 4,324,765 - +1.3 4,324,836 + PS417_19915 0.16 -1.1 4,324,837 - PS417_19915 0.17 +0.4 4,324,984 - PS417_19915 0.35 -0.5 4,325,001 + PS417_19915 0.37 +0.9 4,325,002 - PS417_19915 0.37 +0.5 4,325,007 + PS417_19915 0.38 -1.7 4,325,064 + PS417_19915 0.45 -0.5 4,325,250 + PS417_19915 0.68 -0.3 4,325,250 + PS417_19915 0.68 -1.8 4,325,250 + PS417_19915 0.68 +0.1 4,325,250 + PS417_19915 0.68 -0.1 4,325,250 + PS417_19915 0.68 +0.2 4,325,250 + PS417_19915 0.68 -1.6 4,325,251 - PS417_19915 0.68 -0.8 4,325,251 - PS417_19915 0.68 +0.4 4,325,322 + PS417_19915 0.76 -0.0 4,325,322 + PS417_19915 0.76 +0.5 4,325,322 + PS417_19915 0.76 +0.1 4,325,322 + +1.8 4,325,323 - PS417_19915 0.77 -0.8 4,325,323 - PS417_19915 0.77 -0.2 4,325,560 + -1.6 4,325,561 - -0.3 4,325,565 + +0.2 4,325,565 + -0.1 4,325,619 - -1.5 4,325,619 - -0.9
Or see this region's nucleotide sequence