Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16815 and PS417_16820 are separated by 154 nucleotides PS417_16820 and PS417_16825 are separated by 92 nucleotides PS417_16825 and PS417_16830 are separated by 210 nucleotides
PS417_16815: PS417_16815 - acyl-CoA dehydrogenase, at 3,667,467 to 3,668,663
_16815
PS417_16820: PS417_16820 - Cro/Cl family transcriptional regulator, at 3,668,818 to 3,669,381
_16820
PS417_16825: PS417_16825 - membrane protein, at 3,669,474 to 3,670,403
_16825
PS417_16830: PS417_16830 - monovalent cation/H+ antiporter subunit A, at 3,670,614 to 3,673,535
_16830
Position (kb)
3669
3670
3671 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3668.500 kb on - strand, within PS417_16815 at 3668.633 kb on + strand at 3668.772 kb on + strand at 3668.971 kb on + strand, within PS417_16820 at 3669.801 kb on + strand, within PS417_16825 at 3669.802 kb on - strand, within PS417_16825 at 3669.802 kb on - strand, within PS417_16825 at 3669.802 kb on - strand, within PS417_16825 at 3669.802 kb on - strand, within PS417_16825 at 3669.838 kb on + strand, within PS417_16825 at 3670.505 kb on + strand at 3670.579 kb on - strand at 3670.605 kb on + strand at 3670.605 kb on + strand at 3670.613 kb on + strand at 3670.613 kb on + strand at 3670.625 kb on - strand at 3670.688 kb on + strand at 3670.733 kb on + strand at 3670.896 kb on + strand at 3670.906 kb on - strand at 3670.908 kb on + strand, within PS417_16830 at 3670.908 kb on + strand, within PS417_16830 at 3670.908 kb on + strand, within PS417_16830 at 3670.909 kb on - strand, within PS417_16830 at 3670.909 kb on - strand, within PS417_16830 at 3670.909 kb on - strand, within PS417_16830 at 3670.909 kb on - strand, within PS417_16830 at 3670.909 kb on - strand, within PS417_16830 at 3671.095 kb on - strand, within PS417_16830 at 3671.095 kb on - strand, within PS417_16830 at 3671.095 kb on - strand, within PS417_16830 at 3671.175 kb on + strand, within PS417_16830 at 3671.176 kb on - strand, within PS417_16830 at 3671.341 kb on - strand, within PS417_16830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,668,500 - PS417_16815 0.86 +0.3 3,668,633 + -0.1 3,668,772 + +0.2 3,668,971 + PS417_16820 0.27 +1.5 3,669,801 + PS417_16825 0.35 -0.6 3,669,802 - PS417_16825 0.35 -2.6 3,669,802 - PS417_16825 0.35 -1.4 3,669,802 - PS417_16825 0.35 -1.9 3,669,802 - PS417_16825 0.35 -0.5 3,669,838 + PS417_16825 0.39 -0.2 3,670,505 + +0.5 3,670,579 - +0.1 3,670,605 + -0.6 3,670,605 + +0.1 3,670,613 + -0.3 3,670,613 + -1.7 3,670,625 - +0.4 3,670,688 + -0.1 3,670,733 + -1.4 3,670,896 + +0.2 3,670,906 - -1.1 3,670,908 + PS417_16830 0.10 +1.8 3,670,908 + PS417_16830 0.10 -0.6 3,670,908 + PS417_16830 0.10 +0.7 3,670,909 - PS417_16830 0.10 -0.5 3,670,909 - PS417_16830 0.10 +1.3 3,670,909 - PS417_16830 0.10 -0.7 3,670,909 - PS417_16830 0.10 +0.0 3,670,909 - PS417_16830 0.10 -0.5 3,671,095 - PS417_16830 0.16 +0.2 3,671,095 - PS417_16830 0.16 -2.2 3,671,095 - PS417_16830 0.16 -0.6 3,671,175 + PS417_16830 0.19 +0.1 3,671,176 - PS417_16830 0.19 +0.1 3,671,341 - PS417_16830 0.25 -0.5
Or see this region's nucleotide sequence