Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16550 and PS417_16555 are separated by 83 nucleotides PS417_16555 and PS417_16560 overlap by 8 nucleotides PS417_16560 and PS417_16565 are separated by 231 nucleotides
PS417_16550: PS417_16550 - adenylosuccinate lyase, at 3,616,138 to 3,617,508
_16550
PS417_16555: PS417_16555 - cupin, at 3,617,592 to 3,618,758
_16555
PS417_16560: PS417_16560 - GNAT family acetyltransferase, at 3,618,751 to 3,619,173
_16560
PS417_16565: PS417_16565 - secretin, at 3,619,405 to 3,620,142
_16565
Position (kb)
3617
3618
3619 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3618.159 kb on + strand, within PS417_16555 at 3618.213 kb on + strand, within PS417_16555 at 3618.304 kb on - strand, within PS417_16555 at 3618.767 kb on + strand at 3618.767 kb on + strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.829 kb on - strand, within PS417_16560 at 3619.076 kb on - strand, within PS417_16560 at 3619.091 kb on - strand, within PS417_16560 at 3619.240 kb on + strand at 3619.240 kb on + strand at 3619.240 kb on + strand at 3619.280 kb on + strand at 3619.280 kb on + strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.372 kb on - strand at 3619.372 kb on - strand at 3619.720 kb on + strand, within PS417_16565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,618,159 + PS417_16555 0.49 -2.8 3,618,213 + PS417_16555 0.53 -1.1 3,618,304 - PS417_16555 0.61 -0.6 3,618,767 + -1.6 3,618,767 + -0.3 3,618,768 - -0.6 3,618,768 - -0.8 3,618,768 - -0.7 3,618,768 - -1.8 3,618,768 - -0.8 3,618,829 - PS417_16560 0.18 -3.0 3,619,076 - PS417_16560 0.77 +3.7 3,619,091 - PS417_16560 0.80 +0.1 3,619,240 + -1.2 3,619,240 + -0.2 3,619,240 + -2.3 3,619,280 + -0.5 3,619,280 + -0.8 3,619,281 - -2.2 3,619,281 - -0.5 3,619,281 - -0.4 3,619,281 - +0.1 3,619,281 - -3.1 3,619,372 - +0.5 3,619,372 - -1.6 3,619,720 + PS417_16565 0.43 +0.5
Or see this region's nucleotide sequence