Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16165 and PS417_16170 are separated by 22 nucleotides PS417_16170 and PS417_16175 overlap by 34 nucleotides PS417_16175 and PS417_16180 are separated by 225 nucleotides
PS417_16165: PS417_16165 - ABC transporter substrate-binding protein, at 3,544,793 to 3,545,737
_16165
PS417_16170: PS417_16170 - hypothetical protein, at 3,545,760 to 3,546,167
_16170
PS417_16175: PS417_16175 - porin, at 3,546,134 to 3,546,889
_16175
PS417_16180: PS417_16180 - MFS transporter, at 3,547,115 to 3,548,299
_16180
Position (kb)
3546
3547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3545.222 kb on + strand at 3545.420 kb on + strand, within PS417_16165 at 3545.421 kb on - strand, within PS417_16165 at 3545.430 kb on - strand at 3545.432 kb on + strand, within PS417_16165 at 3545.433 kb on - strand, within PS417_16165 at 3545.487 kb on - strand, within PS417_16165 at 3545.548 kb on - strand, within PS417_16165 at 3545.577 kb on + strand, within PS417_16165 at 3545.688 kb on + strand at 3545.688 kb on + strand at 3545.753 kb on - strand at 3545.802 kb on + strand, within PS417_16170 at 3546.152 kb on - strand at 3546.152 kb on - strand at 3546.349 kb on + strand, within PS417_16175 at 3546.350 kb on - strand, within PS417_16175 at 3546.350 kb on - strand, within PS417_16175 at 3546.535 kb on - strand, within PS417_16175 at 3546.591 kb on + strand, within PS417_16175 at 3546.592 kb on - strand, within PS417_16175 at 3546.593 kb on + strand at 3546.640 kb on - strand, within PS417_16175 at 3546.663 kb on - strand, within PS417_16175 at 3546.977 kb on - strand at 3547.402 kb on - strand, within PS417_16180 at 3547.609 kb on - strand, within PS417_16180 at 3547.609 kb on - strand, within PS417_16180 at 3547.656 kb on + strand, within PS417_16180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,545,222 + -0.3 3,545,420 + PS417_16165 0.66 -0.8 3,545,421 - PS417_16165 0.66 -0.3 3,545,430 - +0.3 3,545,432 + PS417_16165 0.68 -1.2 3,545,433 - PS417_16165 0.68 -0.0 3,545,487 - PS417_16165 0.73 +0.2 3,545,548 - PS417_16165 0.80 -3.7 3,545,577 + PS417_16165 0.83 +0.0 3,545,688 + +0.9 3,545,688 + -1.7 3,545,753 - -0.2 3,545,802 + PS417_16170 0.10 +0.3 3,546,152 - -0.2 3,546,152 - +0.0 3,546,349 + PS417_16175 0.28 +0.2 3,546,350 - PS417_16175 0.29 -0.2 3,546,350 - PS417_16175 0.29 -0.6 3,546,535 - PS417_16175 0.53 -2.7 3,546,591 + PS417_16175 0.60 -1.4 3,546,592 - PS417_16175 0.61 -0.6 3,546,593 + +2.1 3,546,640 - PS417_16175 0.67 +0.1 3,546,663 - PS417_16175 0.70 -1.1 3,546,977 - -0.0 3,547,402 - PS417_16180 0.24 -1.1 3,547,609 - PS417_16180 0.42 +0.7 3,547,609 - PS417_16180 0.42 -0.1 3,547,656 + PS417_16180 0.46 +0.4
Or see this region's nucleotide sequence