Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15415 and PS417_15420 are separated by 86 nucleotides PS417_15420 and PS417_15425 are separated by 214 nucleotides PS417_15425 and PS417_15430 are separated by 170 nucleotides
PS417_15415: PS417_15415 - membrane protein, at 3,378,647 to 3,379,801
_15415
PS417_15420: PS417_15420 - hypothetical protein, at 3,379,888 to 3,380,496
_15420
PS417_15425: PS417_15425 - acetyltransferase, at 3,380,711 to 3,381,169
_15425
PS417_15430: PS417_15430 - porin, at 3,381,340 to 3,382,659
_15430
Position (kb)
3379
3380
3381 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3379.007 kb on + strand, within PS417_15415 at 3379.008 kb on - strand, within PS417_15415 at 3379.412 kb on + strand, within PS417_15415 at 3379.593 kb on - strand, within PS417_15415 at 3379.607 kb on + strand, within PS417_15415 at 3379.608 kb on - strand, within PS417_15415 at 3379.608 kb on - strand, within PS417_15415 at 3379.608 kb on - strand, within PS417_15415 at 3379.626 kb on - strand, within PS417_15415 at 3379.667 kb on + strand, within PS417_15415 at 3379.668 kb on - strand, within PS417_15415 at 3379.794 kb on - strand at 3379.859 kb on - strand at 3379.910 kb on + strand at 3379.910 kb on + strand at 3379.911 kb on - strand at 3379.911 kb on - strand at 3379.911 kb on - strand at 3379.978 kb on - strand, within PS417_15420 at 3380.030 kb on + strand, within PS417_15420 at 3380.030 kb on + strand at 3380.031 kb on - strand, within PS417_15420 at 3380.031 kb on - strand, within PS417_15420 at 3380.267 kb on + strand, within PS417_15420 at 3380.267 kb on + strand, within PS417_15420 at 3380.268 kb on - strand, within PS417_15420 at 3380.276 kb on + strand, within PS417_15420 at 3380.277 kb on - strand, within PS417_15420 at 3380.300 kb on + strand, within PS417_15420 at 3380.300 kb on + strand, within PS417_15420 at 3380.300 kb on + strand, within PS417_15420 at 3380.301 kb on - strand, within PS417_15420 at 3380.301 kb on - strand, within PS417_15420 at 3380.301 kb on - strand, within PS417_15420 at 3380.301 kb on - strand, within PS417_15420 at 3380.462 kb on + strand at 3380.462 kb on + strand at 3380.462 kb on + strand at 3380.463 kb on - strand at 3380.510 kb on + strand at 3380.530 kb on + strand at 3380.631 kb on + strand at 3380.631 kb on + strand at 3380.632 kb on - strand at 3380.744 kb on - strand at 3380.972 kb on + strand, within PS417_15425 at 3380.973 kb on - strand, within PS417_15425 at 3380.976 kb on + strand, within PS417_15425 at 3380.976 kb on + strand, within PS417_15425 at 3380.977 kb on - strand, within PS417_15425 at 3381.060 kb on + strand, within PS417_15425 at 3381.099 kb on - strand, within PS417_15425 at 3381.342 kb on - strand at 3381.364 kb on + strand at 3381.467 kb on + strand at 3381.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,379,007 + PS417_15415 0.31 +3.4 3,379,008 - PS417_15415 0.31 -0.3 3,379,412 + PS417_15415 0.66 +0.7 3,379,593 - PS417_15415 0.82 +0.4 3,379,607 + PS417_15415 0.83 -1.0 3,379,608 - PS417_15415 0.83 +0.9 3,379,608 - PS417_15415 0.83 -0.1 3,379,608 - PS417_15415 0.83 +0.1 3,379,626 - PS417_15415 0.85 -2.1 3,379,667 + PS417_15415 0.88 -0.4 3,379,668 - PS417_15415 0.88 -1.6 3,379,794 - -1.5 3,379,859 - +0.3 3,379,910 + +0.0 3,379,910 + -1.0 3,379,911 - +0.2 3,379,911 - -0.8 3,379,911 - -1.5 3,379,978 - PS417_15420 0.15 -0.4 3,380,030 + PS417_15420 0.23 +0.2 3,380,030 + -0.3 3,380,031 - PS417_15420 0.23 +1.4 3,380,031 - PS417_15420 0.23 +0.6 3,380,267 + PS417_15420 0.62 +0.6 3,380,267 + PS417_15420 0.62 -0.6 3,380,268 - PS417_15420 0.62 -0.0 3,380,276 + PS417_15420 0.64 +0.6 3,380,277 - PS417_15420 0.64 +1.6 3,380,300 + PS417_15420 0.68 +0.1 3,380,300 + PS417_15420 0.68 -0.6 3,380,300 + PS417_15420 0.68 +0.6 3,380,301 - PS417_15420 0.68 -0.6 3,380,301 - PS417_15420 0.68 +0.2 3,380,301 - PS417_15420 0.68 +0.3 3,380,301 - PS417_15420 0.68 +0.2 3,380,462 + -1.0 3,380,462 + +0.9 3,380,462 + -1.5 3,380,463 - -0.5 3,380,510 + -0.4 3,380,530 + +0.7 3,380,631 + +0.8 3,380,631 + -0.9 3,380,632 - -2.7 3,380,744 - -0.0 3,380,972 + PS417_15425 0.57 -0.6 3,380,973 - PS417_15425 0.57 +0.6 3,380,976 + PS417_15425 0.58 +1.0 3,380,976 + PS417_15425 0.58 +0.3 3,380,977 - PS417_15425 0.58 +0.3 3,381,060 + PS417_15425 0.76 +1.7 3,381,099 - PS417_15425 0.85 +0.5 3,381,342 - +0.4 3,381,364 + +0.2 3,381,467 + -0.6 3,381,468 - +0.7
Or see this region's nucleotide sequence