Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12925 and PS417_12930 are separated by 113 nucleotides PS417_12930 and PS417_12935 are separated by 292 nucleotides PS417_12935 and PS417_12940 overlap by 4 nucleotides
PS417_12925: PS417_12925 - serine dehydratase, at 2,826,765 to 2,827,730
_12925
PS417_12930: PS417_12930 - LpxA family transferase, at 2,827,844 to 2,828,473
_12930
PS417_12935: PS417_12935 - RNA polymerase sigma factor, at 2,828,766 to 2,829,284
_12935
PS417_12940: PS417_12940 - sugar ABC transporter substrate-binding protein, at 2,829,281 to 2,830,207
_12940
Position (kb)
2827
2828
2829 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2827.010 kb on - strand, within PS417_12925 at 2827.010 kb on - strand at 2827.129 kb on + strand, within PS417_12925 at 2827.130 kb on - strand, within PS417_12925 at 2827.372 kb on - strand at 2827.374 kb on + strand, within PS417_12925 at 2827.374 kb on + strand, within PS417_12925 at 2827.476 kb on + strand, within PS417_12925 at 2827.795 kb on - strand at 2827.863 kb on - strand at 2827.909 kb on + strand, within PS417_12930 at 2827.982 kb on + strand, within PS417_12930 at 2827.982 kb on + strand at 2827.982 kb on + strand, within PS417_12930 at 2827.983 kb on + strand, within PS417_12930 at 2827.983 kb on - strand, within PS417_12930 at 2827.983 kb on - strand, within PS417_12930 at 2828.224 kb on + strand, within PS417_12930 at 2828.465 kb on + strand at 2828.466 kb on - strand at 2828.466 kb on - strand at 2828.537 kb on + strand at 2828.606 kb on - strand at 2828.631 kb on + strand at 2828.669 kb on + strand at 2828.669 kb on + strand at 2828.780 kb on - strand at 2828.780 kb on - strand at 2828.964 kb on + strand, within PS417_12935 at 2829.031 kb on - strand, within PS417_12935 at 2829.031 kb on - strand, within PS417_12935 at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.242 kb on + strand at 2829.242 kb on + strand at 2829.243 kb on - strand at 2829.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,827,010 - PS417_12925 0.25 +2.0 2,827,010 - -0.5 2,827,129 + PS417_12925 0.38 +2.0 2,827,130 - PS417_12925 0.38 -2.0 2,827,372 - -0.1 2,827,374 + PS417_12925 0.63 +0.1 2,827,374 + PS417_12925 0.63 +0.2 2,827,476 + PS417_12925 0.74 -0.3 2,827,795 - +0.8 2,827,863 - -0.3 2,827,909 + PS417_12930 0.10 +0.3 2,827,982 + PS417_12930 0.22 +0.5 2,827,982 + -1.3 2,827,982 + PS417_12930 0.22 -0.9 2,827,983 + PS417_12930 0.22 -0.6 2,827,983 - PS417_12930 0.22 -0.4 2,827,983 - PS417_12930 0.22 -0.9 2,828,224 + PS417_12930 0.60 -1.0 2,828,465 + -1.5 2,828,466 - +0.6 2,828,466 - -0.5 2,828,537 + -2.5 2,828,606 - +2.2 2,828,631 + -1.5 2,828,669 + +0.1 2,828,669 + +0.1 2,828,780 - +0.8 2,828,780 - +0.7 2,828,964 + PS417_12935 0.38 -0.6 2,829,031 - PS417_12935 0.51 -1.6 2,829,031 - PS417_12935 0.51 -0.7 2,829,240 + -0.4 2,829,240 + +0.4 2,829,240 + +2.0 2,829,240 + -0.5 2,829,242 + -0.3 2,829,242 + -0.4 2,829,243 - +0.2 2,829,255 + -0.5
Or see this region's nucleotide sequence