Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12915 and PS417_12920 overlap by 13 nucleotides PS417_12920 and PS417_12925 overlap by 4 nucleotides PS417_12925 and PS417_12930 are separated by 113 nucleotides
PS417_12915: PS417_12915 - DNA-binding protein, at 2,825,774 to 2,826,400
_12915
PS417_12920: PS417_12920 - endoribonuclease, at 2,826,388 to 2,826,768
_12920
PS417_12925: PS417_12925 - serine dehydratase, at 2,826,765 to 2,827,730
_12925
PS417_12930: PS417_12930 - LpxA family transferase, at 2,827,844 to 2,828,473
_12930
Position (kb)
2826
2827
2828 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2825.783 kb on - strand at 2825.783 kb on - strand at 2826.083 kb on + strand, within PS417_12915 at 2826.084 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.384 kb on - strand at 2826.389 kb on + strand at 2826.389 kb on + strand at 2826.398 kb on + strand at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.598 kb on + strand, within PS417_12920 at 2826.689 kb on + strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.793 kb on + strand at 2826.794 kb on - strand at 2827.010 kb on - strand, within PS417_12925 at 2827.010 kb on - strand at 2827.129 kb on + strand, within PS417_12925 at 2827.130 kb on - strand, within PS417_12925 at 2827.372 kb on - strand at 2827.374 kb on + strand, within PS417_12925 at 2827.374 kb on + strand, within PS417_12925 at 2827.476 kb on + strand, within PS417_12925 at 2827.795 kb on - strand at 2827.863 kb on - strand at 2827.909 kb on + strand, within PS417_12930 at 2827.982 kb on + strand, within PS417_12930 at 2827.982 kb on + strand at 2827.982 kb on + strand, within PS417_12930 at 2827.983 kb on + strand, within PS417_12930 at 2827.983 kb on - strand, within PS417_12930 at 2827.983 kb on - strand, within PS417_12930 at 2828.224 kb on + strand, within PS417_12930 at 2828.465 kb on + strand at 2828.466 kb on - strand at 2828.466 kb on - strand at 2828.537 kb on + strand at 2828.606 kb on - strand at 2828.631 kb on + strand at 2828.669 kb on + strand at 2828.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,825,783 - -1.1 2,825,783 - +0.3 2,826,083 + PS417_12915 0.49 -0.6 2,826,084 - PS417_12915 0.49 -0.3 2,826,289 - PS417_12915 0.82 -0.3 2,826,289 - PS417_12915 0.82 -0.1 2,826,384 - +0.1 2,826,389 + -1.2 2,826,389 + +0.3 2,826,398 + -0.7 2,826,539 + PS417_12920 0.40 -0.6 2,826,539 + PS417_12920 0.40 -0.1 2,826,539 + PS417_12920 0.40 +0.5 2,826,540 - PS417_12920 0.40 -1.1 2,826,540 - PS417_12920 0.40 +0.7 2,826,540 - PS417_12920 0.40 -0.2 2,826,598 + PS417_12920 0.55 +0.3 2,826,689 + PS417_12920 0.79 +2.4 2,826,690 - PS417_12920 0.79 -1.6 2,826,690 - PS417_12920 0.79 -0.8 2,826,690 - PS417_12920 0.79 +0.2 2,826,690 - PS417_12920 0.79 +1.1 2,826,793 + -3.6 2,826,794 - +0.7 2,827,010 - PS417_12925 0.25 +2.0 2,827,010 - -0.5 2,827,129 + PS417_12925 0.38 +2.0 2,827,130 - PS417_12925 0.38 -2.0 2,827,372 - -0.1 2,827,374 + PS417_12925 0.63 +0.1 2,827,374 + PS417_12925 0.63 +0.2 2,827,476 + PS417_12925 0.74 -0.3 2,827,795 - +0.8 2,827,863 - -0.3 2,827,909 + PS417_12930 0.10 +0.3 2,827,982 + PS417_12930 0.22 +0.5 2,827,982 + -1.3 2,827,982 + PS417_12930 0.22 -0.9 2,827,983 + PS417_12930 0.22 -0.6 2,827,983 - PS417_12930 0.22 -0.4 2,827,983 - PS417_12930 0.22 -0.9 2,828,224 + PS417_12930 0.60 -1.0 2,828,465 + -1.5 2,828,466 - +0.6 2,828,466 - -0.5 2,828,537 + -2.5 2,828,606 - +2.2 2,828,631 + -1.5 2,828,669 + +0.1 2,828,669 + +0.1
Or see this region's nucleotide sequence