Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12400 and PS417_12405 are separated by 158 nucleotides PS417_12405 and PS417_12410 are separated by 52 nucleotides PS417_12410 and PS417_12415 are separated by 15 nucleotides
PS417_12400: PS417_12400 - oxidoreductase, at 2,697,550 to 2,698,596
_12400
PS417_12405: PS417_12405 - DNA-binding protein, at 2,698,755 to 2,699,471
_12405
PS417_12410: PS417_12410 - ketosteroid isomerase, at 2,699,524 to 2,699,859
_12410
PS417_12415: PS417_12415 - ATPase, at 2,699,875 to 2,700,978
_12415
Position (kb)
2698
2699
2700 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2698.040 kb on + strand, within PS417_12400 at 2698.108 kb on - strand, within PS417_12400 at 2698.346 kb on + strand, within PS417_12400 at 2698.347 kb on - strand, within PS417_12400 at 2698.347 kb on - strand, within PS417_12400 at 2698.347 kb on - strand, within PS417_12400 at 2698.425 kb on - strand, within PS417_12400 at 2698.555 kb on + strand at 2698.555 kb on + strand at 2698.556 kb on - strand at 2698.556 kb on - strand at 2698.596 kb on + strand at 2698.597 kb on - strand at 2698.774 kb on + strand at 2698.774 kb on + strand at 2698.929 kb on + strand, within PS417_12405 at 2698.929 kb on + strand, within PS417_12405 at 2698.930 kb on - strand, within PS417_12405 at 2698.930 kb on - strand, within PS417_12405 at 2699.454 kb on - strand at 2699.486 kb on - strand at 2699.486 kb on - strand at 2699.508 kb on - strand at 2699.508 kb on - strand at 2699.530 kb on - strand at 2699.829 kb on + strand at 2699.858 kb on - strand at 2699.858 kb on - strand at 2699.858 kb on - strand at 2699.903 kb on - strand at 2699.990 kb on + strand, within PS417_12415 at 2700.073 kb on + strand, within PS417_12415 at 2700.341 kb on - strand, within PS417_12415 at 2700.341 kb on - strand, within PS417_12415 at 2700.341 kb on - strand, within PS417_12415 at 2700.389 kb on + strand, within PS417_12415 at 2700.390 kb on - strand, within PS417_12415 at 2700.390 kb on - strand, within PS417_12415 at 2700.399 kb on + strand, within PS417_12415 at 2700.399 kb on + strand, within PS417_12415 at 2700.399 kb on + strand, within PS417_12415 at 2700.399 kb on + strand, within PS417_12415 at 2700.399 kb on + strand, within PS417_12415 at 2700.399 kb on + strand, within PS417_12415 at 2700.400 kb on - strand, within PS417_12415 at 2700.400 kb on - strand at 2700.400 kb on - strand, within PS417_12415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,698,040 + PS417_12400 0.47 -1.3 2,698,108 - PS417_12400 0.53 -1.4 2,698,346 + PS417_12400 0.76 -3.5 2,698,347 - PS417_12400 0.76 +4.2 2,698,347 - PS417_12400 0.76 -1.2 2,698,347 - PS417_12400 0.76 -1.5 2,698,425 - PS417_12400 0.84 -0.9 2,698,555 + -0.4 2,698,555 + -0.1 2,698,556 - +1.5 2,698,556 - -0.0 2,698,596 + +0.2 2,698,597 - -0.0 2,698,774 + -0.3 2,698,774 + -0.9 2,698,929 + PS417_12405 0.24 +0.5 2,698,929 + PS417_12405 0.24 +0.5 2,698,930 - PS417_12405 0.24 -0.5 2,698,930 - PS417_12405 0.24 -1.4 2,699,454 - +0.4 2,699,486 - -0.8 2,699,486 - -0.3 2,699,508 - -0.3 2,699,508 - +0.2 2,699,530 - -1.0 2,699,829 + +0.1 2,699,858 - +3.0 2,699,858 - +0.8 2,699,858 - -0.3 2,699,903 - +0.9 2,699,990 + PS417_12415 0.10 -0.4 2,700,073 + PS417_12415 0.18 +0.1 2,700,341 - PS417_12415 0.42 -0.4 2,700,341 - PS417_12415 0.42 +0.3 2,700,341 - PS417_12415 0.42 -2.1 2,700,389 + PS417_12415 0.47 -0.6 2,700,390 - PS417_12415 0.47 -0.7 2,700,390 - PS417_12415 0.47 -0.4 2,700,399 + PS417_12415 0.47 -0.7 2,700,399 + PS417_12415 0.47 -0.1 2,700,399 + PS417_12415 0.47 -0.2 2,700,399 + PS417_12415 0.47 +1.7 2,700,399 + PS417_12415 0.47 -0.1 2,700,399 + PS417_12415 0.47 +0.2 2,700,400 - PS417_12415 0.48 -0.3 2,700,400 - +1.7 2,700,400 - PS417_12415 0.48 +0.7
Or see this region's nucleotide sequence