Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10530 and PS417_10535 are separated by 59 nucleotides PS417_10535 and PS417_10540 are separated by 546 nucleotides PS417_10540 and PS417_10545 are separated by 8 nucleotides PS417_10545 and PS417_10550 are separated by 131 nucleotides
PS417_10530: PS417_10530 - LysR family transcriptional regulator, at 2,246,562 to 2,247,467
_10530
PS417_10535: PS417_10535 - hypothetical protein, at 2,247,527 to 2,247,856
_10535
PS417_10540: PS417_10540 - NAD-dependent deacetylase, at 2,248,403 to 2,249,248
_10540
PS417_10545: PS417_10545 - histidine kinase, at 2,249,257 to 2,249,697
_10545
PS417_10550: PS417_10550 - DNA topoisomerase III, at 2,249,829 to 2,251,778
_10550
Position (kb)
2248
2249
2250 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2247.419 kb on + strand at 2247.463 kb on + strand at 2247.463 kb on + strand at 2247.464 kb on - strand at 2247.465 kb on + strand at 2247.465 kb on + strand at 2247.465 kb on + strand at 2247.466 kb on - strand at 2247.496 kb on - strand at 2247.534 kb on + strand at 2247.534 kb on + strand at 2247.535 kb on - strand at 2247.539 kb on - strand at 2247.587 kb on - strand, within PS417_10535 at 2247.590 kb on - strand, within PS417_10535 at 2247.648 kb on + strand, within PS417_10535 at 2247.649 kb on - strand, within PS417_10535 at 2247.667 kb on - strand, within PS417_10535 at 2247.860 kb on + strand at 2247.886 kb on - strand at 2247.911 kb on + strand at 2247.911 kb on + strand at 2247.911 kb on + strand at 2247.911 kb on + strand at 2247.912 kb on - strand at 2247.912 kb on - strand at 2247.912 kb on - strand at 2247.912 kb on - strand at 2247.989 kb on - strand at 2248.159 kb on - strand at 2248.210 kb on + strand at 2248.210 kb on + strand at 2248.246 kb on + strand at 2248.247 kb on - strand at 2248.251 kb on - strand at 2248.251 kb on - strand at 2248.251 kb on - strand at 2248.377 kb on + strand at 2248.378 kb on - strand at 2248.398 kb on - strand at 2248.557 kb on + strand, within PS417_10540 at 2248.661 kb on + strand at 2248.662 kb on - strand, within PS417_10540 at 2249.082 kb on + strand, within PS417_10540 at 2249.082 kb on + strand, within PS417_10540 at 2249.227 kb on + strand at 2249.227 kb on + strand at 2249.227 kb on + strand at 2249.227 kb on + strand at 2249.228 kb on - strand at 2249.273 kb on + strand at 2249.274 kb on - strand at 2249.274 kb on - strand at 2249.274 kb on - strand at 2249.274 kb on - strand at 2249.274 kb on - strand at 2249.431 kb on - strand, within PS417_10545 at 2249.741 kb on - strand at 2249.751 kb on - strand at 2249.894 kb on + strand at 2249.894 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,247,419 + -0.1 2,247,463 + -0.1 2,247,463 + -0.3 2,247,464 - +1.6 2,247,465 + -0.9 2,247,465 + -0.1 2,247,465 + -1.4 2,247,466 - -0.3 2,247,496 - -0.7 2,247,534 + +0.7 2,247,534 + -1.1 2,247,535 - -1.5 2,247,539 - -0.1 2,247,587 - PS417_10535 0.18 -1.4 2,247,590 - PS417_10535 0.19 -0.3 2,247,648 + PS417_10535 0.37 +1.1 2,247,649 - PS417_10535 0.37 -0.7 2,247,667 - PS417_10535 0.42 +0.3 2,247,860 + -0.0 2,247,886 - -0.3 2,247,911 + -1.2 2,247,911 + -2.1 2,247,911 + +0.5 2,247,911 + -0.6 2,247,912 - +0.3 2,247,912 - -1.1 2,247,912 - -0.9 2,247,912 - +0.3 2,247,989 - +0.5 2,248,159 - +0.1 2,248,210 + -0.2 2,248,210 + -0.1 2,248,246 + -0.9 2,248,247 - -0.1 2,248,251 - -0.3 2,248,251 - -1.0 2,248,251 - -0.4 2,248,377 + -0.3 2,248,378 - -0.2 2,248,398 - -1.7 2,248,557 + PS417_10540 0.18 +1.2 2,248,661 + +2.8 2,248,662 - PS417_10540 0.31 +1.1 2,249,082 + PS417_10540 0.80 +0.6 2,249,082 + PS417_10540 0.80 -0.2 2,249,227 + -0.1 2,249,227 + +1.5 2,249,227 + -0.5 2,249,227 + -0.8 2,249,228 - -0.2 2,249,273 + -0.6 2,249,274 - -0.3 2,249,274 - -0.3 2,249,274 - -0.2 2,249,274 - +0.1 2,249,274 - +0.9 2,249,431 - PS417_10545 0.39 -0.8 2,249,741 - +1.3 2,249,751 - -0.3 2,249,894 + +0.5 2,249,894 + -3.0
Or see this region's nucleotide sequence