Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09885 and PS417_09890 are separated by 208 nucleotides PS417_09890 and PS417_09895 are separated by 119 nucleotides PS417_09895 and PS417_09900 are separated by 69 nucleotides PS417_09900 and PS417_09905 are separated by 2 nucleotides PS417_09905 and PS417_09910 overlap by 4 nucleotides
PS417_09885: PS417_09885 - hypothetical protein, at 2,139,015 to 2,139,572
_09885
PS417_09890: PS417_09890 - hypothetical protein, at 2,139,781 to 2,140,014
_09890
PS417_09895: PS417_09895 - hypothetical protein, at 2,140,134 to 2,140,460
_09895
PS417_09900: PS417_09900 - hypothetical protein, at 2,140,530 to 2,141,123
_09900
PS417_09905: PS417_09905 - exonuclease, at 2,141,126 to 2,141,788
_09905
PS417_09910: PS417_09910 - gifsy-1 prophage protein, at 2,141,785 to 2,142,753
_09910
Position (kb)
2140
2141
2142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2139.546 kb on - strand at 2139.546 kb on - strand at 2139.546 kb on - strand at 2139.546 kb on - strand at 2139.570 kb on + strand at 2139.570 kb on + strand at 2139.570 kb on + strand at 2139.605 kb on - strand at 2139.609 kb on + strand at 2139.609 kb on + strand at 2139.609 kb on + strand at 2139.712 kb on + strand at 2139.712 kb on + strand at 2139.782 kb on + strand at 2139.782 kb on + strand at 2139.783 kb on - strand at 2139.783 kb on - strand at 2139.783 kb on - strand at 2139.834 kb on + strand, within PS417_09890 at 2139.834 kb on + strand, within PS417_09890 at 2139.835 kb on - strand, within PS417_09890 at 2139.835 kb on - strand, within PS417_09890 at 2139.835 kb on - strand, within PS417_09890 at 2139.835 kb on - strand, within PS417_09890 at 2139.838 kb on + strand, within PS417_09890 at 2139.868 kb on + strand, within PS417_09890 at 2139.911 kb on + strand, within PS417_09890 at 2139.911 kb on + strand, within PS417_09890 at 2139.912 kb on - strand, within PS417_09890 at 2139.912 kb on - strand, within PS417_09890 at 2139.912 kb on - strand, within PS417_09890 at 2139.915 kb on + strand, within PS417_09890 at 2139.916 kb on - strand, within PS417_09890 at 2139.916 kb on - strand, within PS417_09890 at 2139.946 kb on + strand, within PS417_09890 at 2139.946 kb on + strand, within PS417_09890 at 2139.976 kb on + strand, within PS417_09890 at 2140.094 kb on - strand at 2140.151 kb on - strand at 2140.224 kb on + strand, within PS417_09895 at 2140.224 kb on + strand, within PS417_09895 at 2140.465 kb on + strand at 2140.465 kb on + strand at 2140.466 kb on - strand at 2140.531 kb on + strand at 2140.771 kb on + strand, within PS417_09900 at 2140.772 kb on - strand, within PS417_09900 at 2140.772 kb on - strand, within PS417_09900 at 2140.775 kb on + strand, within PS417_09900 at 2140.789 kb on + strand, within PS417_09900 at 2140.789 kb on + strand, within PS417_09900 at 2140.790 kb on - strand, within PS417_09900 at 2140.860 kb on + strand, within PS417_09900 at 2140.860 kb on + strand, within PS417_09900 at 2140.861 kb on - strand, within PS417_09900 at 2140.861 kb on - strand, within PS417_09900 at 2140.981 kb on + strand, within PS417_09900 at 2140.981 kb on + strand, within PS417_09900 at 2140.981 kb on + strand, within PS417_09900 at 2140.982 kb on - strand at 2141.047 kb on + strand, within PS417_09900 at 2141.047 kb on + strand, within PS417_09900 at 2141.048 kb on - strand, within PS417_09900 at 2141.127 kb on + strand at 2141.128 kb on - strand at 2141.128 kb on - strand at 2141.136 kb on - strand at 2141.235 kb on + strand at 2141.235 kb on + strand, within PS417_09905 at 2141.265 kb on + strand, within PS417_09905 at 2141.266 kb on - strand, within PS417_09905 at 2141.266 kb on - strand, within PS417_09905 at 2141.395 kb on + strand, within PS417_09905 at 2141.395 kb on + strand, within PS417_09905 at 2141.395 kb on + strand, within PS417_09905 at 2141.396 kb on - strand, within PS417_09905 at 2141.396 kb on - strand, within PS417_09905 at 2141.396 kb on - strand, within PS417_09905 at 2141.399 kb on + strand, within PS417_09905 at 2141.399 kb on + strand, within PS417_09905 at 2141.399 kb on + strand, within PS417_09905 at 2141.400 kb on - strand, within PS417_09905 at 2141.400 kb on - strand, within PS417_09905 at 2141.400 kb on - strand, within PS417_09905 at 2141.400 kb on - strand, within PS417_09905 at 2141.448 kb on - strand, within PS417_09905 at 2141.481 kb on + strand, within PS417_09905 at 2141.482 kb on - strand, within PS417_09905 at 2141.599 kb on + strand, within PS417_09905 at 2141.646 kb on + strand, within PS417_09905 at 2141.646 kb on + strand, within PS417_09905 at 2141.646 kb on + strand, within PS417_09905 at 2141.647 kb on - strand, within PS417_09905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,139,546 - +0.6 2,139,546 - -0.1 2,139,546 - +0.0 2,139,546 - -0.7 2,139,570 + -0.4 2,139,570 + -1.8 2,139,570 + +0.3 2,139,605 - +3.1 2,139,609 + +0.5 2,139,609 + -0.2 2,139,609 + -0.3 2,139,712 + -0.7 2,139,712 + +1.1 2,139,782 + -0.3 2,139,782 + -0.2 2,139,783 - +0.6 2,139,783 - +0.8 2,139,783 - -0.9 2,139,834 + PS417_09890 0.23 -0.6 2,139,834 + PS417_09890 0.23 -0.7 2,139,835 - PS417_09890 0.23 +0.4 2,139,835 - PS417_09890 0.23 +0.5 2,139,835 - PS417_09890 0.23 -0.6 2,139,835 - PS417_09890 0.23 -0.2 2,139,838 + PS417_09890 0.24 -0.8 2,139,868 + PS417_09890 0.37 -0.3 2,139,911 + PS417_09890 0.56 -0.3 2,139,911 + PS417_09890 0.56 -0.7 2,139,912 - PS417_09890 0.56 -0.0 2,139,912 - PS417_09890 0.56 +0.2 2,139,912 - PS417_09890 0.56 -1.5 2,139,915 + PS417_09890 0.57 -3.0 2,139,916 - PS417_09890 0.58 -1.2 2,139,916 - PS417_09890 0.58 -1.6 2,139,946 + PS417_09890 0.71 -0.4 2,139,946 + PS417_09890 0.71 +0.9 2,139,976 + PS417_09890 0.83 +0.5 2,140,094 - -0.4 2,140,151 - +0.9 2,140,224 + PS417_09895 0.28 +0.3 2,140,224 + PS417_09895 0.28 -0.5 2,140,465 + -0.2 2,140,465 + -0.5 2,140,466 - +1.3 2,140,531 + -1.0 2,140,771 + PS417_09900 0.41 +0.1 2,140,772 - PS417_09900 0.41 +0.7 2,140,772 - PS417_09900 0.41 -0.1 2,140,775 + PS417_09900 0.41 +0.8 2,140,789 + PS417_09900 0.44 -0.3 2,140,789 + PS417_09900 0.44 -2.4 2,140,790 - PS417_09900 0.44 +1.6 2,140,860 + PS417_09900 0.56 +1.7 2,140,860 + PS417_09900 0.56 -1.3 2,140,861 - PS417_09900 0.56 -0.1 2,140,861 - PS417_09900 0.56 -0.2 2,140,981 + PS417_09900 0.76 +0.9 2,140,981 + PS417_09900 0.76 +1.1 2,140,981 + PS417_09900 0.76 +1.2 2,140,982 - +1.4 2,141,047 + PS417_09900 0.87 -1.8 2,141,047 + PS417_09900 0.87 +2.0 2,141,048 - PS417_09900 0.87 -3.2 2,141,127 + +2.0 2,141,128 - -1.8 2,141,128 - +1.4 2,141,136 - -0.3 2,141,235 + -1.5 2,141,235 + PS417_09905 0.16 -0.7 2,141,265 + PS417_09905 0.21 -1.3 2,141,266 - PS417_09905 0.21 -1.4 2,141,266 - PS417_09905 0.21 -1.1 2,141,395 + PS417_09905 0.41 -2.7 2,141,395 + PS417_09905 0.41 -1.3 2,141,395 + PS417_09905 0.41 -0.1 2,141,396 - PS417_09905 0.41 +0.3 2,141,396 - PS417_09905 0.41 -0.5 2,141,396 - PS417_09905 0.41 +0.3 2,141,399 + PS417_09905 0.41 +0.1 2,141,399 + PS417_09905 0.41 +1.8 2,141,399 + PS417_09905 0.41 -0.2 2,141,400 - PS417_09905 0.41 -1.2 2,141,400 - PS417_09905 0.41 -0.3 2,141,400 - PS417_09905 0.41 +0.6 2,141,400 - PS417_09905 0.41 +1.9 2,141,448 - PS417_09905 0.49 -1.4 2,141,481 + PS417_09905 0.54 -0.3 2,141,482 - PS417_09905 0.54 -0.2 2,141,599 + PS417_09905 0.71 +0.9 2,141,646 + PS417_09905 0.78 +0.5 2,141,646 + PS417_09905 0.78 +0.6 2,141,646 + PS417_09905 0.78 +0.4 2,141,647 - PS417_09905 0.79 +0.5
Or see this region's nucleotide sequence