Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09070 and PS417_09075 are separated by 114 nucleotides PS417_09075 and PS417_09080 are separated by 37 nucleotides
PS417_09070: PS417_09070 - GntR family transcriptional regulator, at 1,974,088 to 1,975,500
_09070
PS417_09075: PS417_09075 - chemotaxis protein, at 1,975,615 to 1,977,582
_09075
PS417_09080: PS417_09080 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 1,977,620 to 1,978,432
_09080
Position (kb)
1975
1976
1977
1978 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1974.994 kb on + strand, within PS417_09070 at 1974.994 kb on + strand, within PS417_09070 at 1974.995 kb on - strand, within PS417_09070 at 1975.037 kb on + strand, within PS417_09070 at 1975.043 kb on - strand, within PS417_09070 at 1975.159 kb on + strand, within PS417_09070 at 1975.212 kb on + strand, within PS417_09070 at 1975.213 kb on - strand, within PS417_09070 at 1975.269 kb on + strand, within PS417_09070 at 1975.269 kb on + strand, within PS417_09070 at 1975.296 kb on - strand, within PS417_09070 at 1975.498 kb on + strand at 1975.689 kb on - strand at 1975.794 kb on - strand at 1975.944 kb on - strand, within PS417_09075 at 1975.944 kb on - strand, within PS417_09075 at 1976.207 kb on + strand, within PS417_09075 at 1976.208 kb on - strand, within PS417_09075 at 1976.208 kb on - strand, within PS417_09075 at 1976.208 kb on - strand, within PS417_09075 at 1976.208 kb on - strand at 1976.208 kb on - strand, within PS417_09075 at 1976.208 kb on - strand, within PS417_09075 at 1976.390 kb on - strand, within PS417_09075 at 1976.399 kb on + strand, within PS417_09075 at 1976.476 kb on + strand, within PS417_09075 at 1977.066 kb on + strand, within PS417_09075 at 1977.067 kb on - strand, within PS417_09075 at 1977.194 kb on + strand, within PS417_09075 at 1977.498 kb on + strand at 1977.558 kb on + strand at 1977.559 kb on - strand at 1977.688 kb on + strand at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.898 kb on - strand, within PS417_09080 at 1977.927 kb on + strand at 1977.927 kb on + strand at 1978.038 kb on + strand, within PS417_09080 at 1978.074 kb on + strand, within PS417_09080 at 1978.075 kb on - strand, within PS417_09080 at 1978.075 kb on - strand, within PS417_09080 at 1978.141 kb on + strand, within PS417_09080 at 1978.350 kb on + strand, within PS417_09080 at 1978.350 kb on + strand, within PS417_09080 at 1978.351 kb on - strand at 1978.391 kb on + strand at 1978.456 kb on + strand at 1978.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,974,994 + PS417_09070 0.64 -1.2 1,974,994 + PS417_09070 0.64 +0.1 1,974,995 - PS417_09070 0.64 +0.1 1,975,037 + PS417_09070 0.67 -0.8 1,975,043 - PS417_09070 0.68 -1.4 1,975,159 + PS417_09070 0.76 +0.1 1,975,212 + PS417_09070 0.80 -1.9 1,975,213 - PS417_09070 0.80 -1.2 1,975,269 + PS417_09070 0.84 +0.2 1,975,269 + PS417_09070 0.84 +0.6 1,975,296 - PS417_09070 0.85 +0.8 1,975,498 + +0.3 1,975,689 - -1.2 1,975,794 - +0.7 1,975,944 - PS417_09075 0.17 +2.1 1,975,944 - PS417_09075 0.17 +0.0 1,976,207 + PS417_09075 0.30 +0.7 1,976,208 - PS417_09075 0.30 -0.5 1,976,208 - PS417_09075 0.30 +0.4 1,976,208 - PS417_09075 0.30 +0.1 1,976,208 - +1.7 1,976,208 - PS417_09075 0.30 +1.7 1,976,208 - PS417_09075 0.30 -0.2 1,976,390 - PS417_09075 0.39 -0.3 1,976,399 + PS417_09075 0.40 +0.1 1,976,476 + PS417_09075 0.44 +1.7 1,977,066 + PS417_09075 0.74 +0.7 1,977,067 - PS417_09075 0.74 +0.2 1,977,194 + PS417_09075 0.80 +0.8 1,977,498 + +0.3 1,977,558 + -0.7 1,977,559 - +1.7 1,977,688 + -0.1 1,977,831 - PS417_09080 0.26 -0.4 1,977,831 - PS417_09080 0.26 -1.3 1,977,831 - PS417_09080 0.26 +1.5 1,977,831 - PS417_09080 0.26 -0.3 1,977,831 - PS417_09080 0.26 -0.6 1,977,898 - PS417_09080 0.34 +0.2 1,977,927 + -0.6 1,977,927 + -0.3 1,978,038 + PS417_09080 0.51 -0.5 1,978,074 + PS417_09080 0.56 -1.8 1,978,075 - PS417_09080 0.56 +0.1 1,978,075 - PS417_09080 0.56 -0.7 1,978,141 + PS417_09080 0.64 -1.2 1,978,350 + PS417_09080 0.90 -1.1 1,978,350 + PS417_09080 0.90 +0.4 1,978,351 - -0.1 1,978,391 + +0.9 1,978,456 + +0.8 1,978,457 - -1.5
Or see this region's nucleotide sequence