Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08955 and PS417_08960 are separated by 64 nucleotides PS417_08960 and PS417_08965 are separated by 140 nucleotides PS417_08965 and PS417_08970 are separated by 95 nucleotides
PS417_08955: PS417_08955 - pimeloyl-CoA dehydrogenase, at 1,949,531 to 1,950,397
_08955
PS417_08960: PS417_08960 - peroxiredoxin, at 1,950,462 to 1,950,878
_08960
PS417_08965: PS417_08965 - 5-oxo-L-prolinase, at 1,951,019 to 1,952,041
_08965
PS417_08970: PS417_08970 - chemotaxis protein, at 1,952,137 to 1,953,762
_08970
Position (kb)
1951
1952
1953 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1950.088 kb on + strand, within PS417_08955 at 1950.089 kb on - strand, within PS417_08955 at 1950.089 kb on - strand, within PS417_08955 at 1950.201 kb on + strand, within PS417_08955 at 1950.201 kb on + strand at 1950.202 kb on - strand, within PS417_08955 at 1950.202 kb on - strand, within PS417_08955 at 1950.202 kb on - strand, within PS417_08955 at 1950.224 kb on - strand, within PS417_08955 at 1950.281 kb on - strand, within PS417_08955 at 1950.281 kb on - strand, within PS417_08955 at 1950.281 kb on - strand, within PS417_08955 at 1950.437 kb on + strand at 1950.619 kb on + strand, within PS417_08960 at 1950.619 kb on + strand, within PS417_08960 at 1950.619 kb on + strand, within PS417_08960 at 1950.619 kb on + strand at 1950.620 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.700 kb on + strand, within PS417_08960 at 1950.749 kb on - strand, within PS417_08960 at 1950.749 kb on - strand, within PS417_08960 at 1950.749 kb on - strand, within PS417_08960 at 1950.824 kb on + strand, within PS417_08960 at 1950.881 kb on + strand at 1950.882 kb on - strand at 1950.918 kb on + strand at 1950.918 kb on + strand at 1950.919 kb on - strand at 1950.919 kb on - strand at 1950.920 kb on + strand at 1950.920 kb on + strand at 1950.920 kb on + strand at 1951.016 kb on - strand at 1951.094 kb on + strand at 1951.254 kb on - strand, within PS417_08965 at 1951.280 kb on + strand, within PS417_08965 at 1951.280 kb on + strand, within PS417_08965 at 1951.281 kb on - strand, within PS417_08965 at 1951.286 kb on + strand, within PS417_08965 at 1951.538 kb on + strand, within PS417_08965 at 1951.539 kb on - strand, within PS417_08965 at 1951.539 kb on - strand, within PS417_08965 at 1951.539 kb on - strand, within PS417_08965 at 1951.616 kb on + strand, within PS417_08965 at 1951.617 kb on - strand, within PS417_08965 at 1951.772 kb on + strand, within PS417_08965 at 1951.773 kb on - strand, within PS417_08965 at 1951.877 kb on - strand, within PS417_08965 at 1952.058 kb on + strand at 1952.142 kb on + strand at 1952.143 kb on - strand at 1952.307 kb on + strand, within PS417_08970 at 1952.307 kb on + strand, within PS417_08970 at 1952.440 kb on + strand, within PS417_08970 at 1952.440 kb on + strand, within PS417_08970 at 1952.456 kb on + strand at 1952.457 kb on - strand, within PS417_08970 at 1952.477 kb on + strand, within PS417_08970 at 1952.478 kb on - strand, within PS417_08970 at 1952.509 kb on + strand, within PS417_08970 at 1952.509 kb on + strand, within PS417_08970 at 1952.688 kb on - strand, within PS417_08970 at 1952.775 kb on + strand, within PS417_08970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,950,088 + PS417_08955 0.64 +0.0 1,950,089 - PS417_08955 0.64 -1.3 1,950,089 - PS417_08955 0.64 -0.2 1,950,201 + PS417_08955 0.77 +1.4 1,950,201 + -0.6 1,950,202 - PS417_08955 0.77 -0.5 1,950,202 - PS417_08955 0.77 -0.7 1,950,202 - PS417_08955 0.77 +3.0 1,950,224 - PS417_08955 0.80 +0.2 1,950,281 - PS417_08955 0.87 -1.8 1,950,281 - PS417_08955 0.87 +0.4 1,950,281 - PS417_08955 0.87 -0.6 1,950,437 + +1.5 1,950,619 + PS417_08960 0.38 +0.5 1,950,619 + PS417_08960 0.38 +0.8 1,950,619 + PS417_08960 0.38 +0.6 1,950,619 + +0.3 1,950,620 - PS417_08960 0.38 +4.0 1,950,661 - PS417_08960 0.48 -0.1 1,950,661 - PS417_08960 0.48 +0.2 1,950,661 - PS417_08960 0.48 +0.4 1,950,661 - PS417_08960 0.48 -1.1 1,950,700 + PS417_08960 0.57 -2.5 1,950,749 - PS417_08960 0.69 -1.3 1,950,749 - PS417_08960 0.69 -3.0 1,950,749 - PS417_08960 0.69 +0.4 1,950,824 + PS417_08960 0.87 -1.1 1,950,881 + +2.3 1,950,882 - -0.2 1,950,918 + +0.1 1,950,918 + -0.3 1,950,919 - +0.4 1,950,919 - +2.2 1,950,920 + -1.5 1,950,920 + +0.3 1,950,920 + +0.7 1,951,016 - +1.0 1,951,094 + -2.0 1,951,254 - PS417_08965 0.23 -0.1 1,951,280 + PS417_08965 0.26 +0.2 1,951,280 + PS417_08965 0.26 -0.0 1,951,281 - PS417_08965 0.26 +0.1 1,951,286 + PS417_08965 0.26 +0.1 1,951,538 + PS417_08965 0.51 +1.2 1,951,539 - PS417_08965 0.51 +0.1 1,951,539 - PS417_08965 0.51 -0.0 1,951,539 - PS417_08965 0.51 -0.3 1,951,616 + PS417_08965 0.58 +0.7 1,951,617 - PS417_08965 0.58 +0.2 1,951,772 + PS417_08965 0.74 -1.0 1,951,773 - PS417_08965 0.74 +0.9 1,951,877 - PS417_08965 0.84 +0.3 1,952,058 + +0.7 1,952,142 + +0.1 1,952,143 - -2.2 1,952,307 + PS417_08970 0.10 -0.1 1,952,307 + PS417_08970 0.10 -2.5 1,952,440 + PS417_08970 0.19 +0.5 1,952,440 + PS417_08970 0.19 +0.8 1,952,456 + -1.3 1,952,457 - PS417_08970 0.20 +1.9 1,952,477 + PS417_08970 0.21 -0.4 1,952,478 - PS417_08970 0.21 -0.3 1,952,509 + PS417_08970 0.23 -0.3 1,952,509 + PS417_08970 0.23 -0.2 1,952,688 - PS417_08970 0.34 +0.3 1,952,775 + PS417_08970 0.39 -0.3
Or see this region's nucleotide sequence