Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08925 and PS417_08930 are separated by 90 nucleotides PS417_08930 and PS417_08935 are separated by 157 nucleotides PS417_08935 and PS417_08940 overlap by 4 nucleotides PS417_08940 and PS417_08945 are separated by 89 nucleotides
PS417_08925: PS417_08925 - membrane protein, at 1,944,434 to 1,945,171
_08925
PS417_08930: PS417_08930 - hypothetical protein, at 1,945,262 to 1,945,513
_08930
PS417_08935: PS417_08935 - thioesterase, at 1,945,671 to 1,946,147
_08935
PS417_08940: PS417_08940 - thioesterase, at 1,946,144 to 1,946,596
_08940
PS417_08945: PS417_08945 - heat shock protein 90, at 1,946,686 to 1,948,590
_08945
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1944.742 kb on + strand, within PS417_08925 at 1944.816 kb on - strand, within PS417_08925 at 1944.816 kb on - strand, within PS417_08925 at 1944.851 kb on + strand, within PS417_08925 at 1944.995 kb on + strand, within PS417_08925 at 1944.995 kb on + strand, within PS417_08925 at 1944.996 kb on - strand, within PS417_08925 at 1945.121 kb on + strand at 1945.121 kb on + strand at 1945.122 kb on - strand at 1945.122 kb on - strand at 1945.169 kb on + strand at 1945.173 kb on + strand at 1945.174 kb on - strand at 1945.217 kb on + strand at 1945.245 kb on + strand at 1945.245 kb on + strand at 1945.245 kb on + strand at 1945.373 kb on - strand, within PS417_08930 at 1945.373 kb on - strand, within PS417_08930 at 1945.499 kb on + strand at 1945.499 kb on + strand at 1945.499 kb on + strand at 1945.500 kb on - strand at 1945.500 kb on - strand at 1945.537 kb on - strand at 1945.537 kb on - strand at 1945.540 kb on + strand at 1945.540 kb on + strand at 1945.572 kb on - strand at 1945.588 kb on + strand at 1945.589 kb on - strand at 1945.592 kb on + strand at 1945.592 kb on + strand at 1945.592 kb on + strand at 1945.592 kb on + strand at 1945.593 kb on - strand at 1945.593 kb on - strand at 1945.593 kb on - strand at 1945.593 kb on - strand at 1945.593 kb on - strand at 1945.594 kb on + strand at 1945.594 kb on + strand at 1945.594 kb on + strand at 1945.595 kb on - strand at 1945.595 kb on - strand at 1945.597 kb on - strand at 1945.597 kb on - strand at 1945.652 kb on + strand at 1945.655 kb on + strand at 1945.775 kb on + strand, within PS417_08935 at 1945.775 kb on + strand, within PS417_08935 at 1945.792 kb on - strand, within PS417_08935 at 1945.892 kb on - strand, within PS417_08935 at 1945.892 kb on - strand, within PS417_08935 at 1945.938 kb on + strand, within PS417_08935 at 1945.938 kb on + strand, within PS417_08935 at 1945.938 kb on + strand, within PS417_08935 at 1945.939 kb on - strand at 1946.031 kb on + strand, within PS417_08935 at 1946.078 kb on + strand, within PS417_08935 at 1946.078 kb on + strand, within PS417_08935 at 1946.079 kb on - strand, within PS417_08935 at 1946.079 kb on - strand, within PS417_08935 at 1946.196 kb on - strand, within PS417_08940 at 1946.299 kb on + strand, within PS417_08940 at 1946.299 kb on + strand, within PS417_08940 at 1946.300 kb on - strand, within PS417_08940 at 1946.300 kb on - strand, within PS417_08940 at 1946.300 kb on - strand, within PS417_08940 at 1946.463 kb on - strand at 1946.768 kb on - strand at 1946.831 kb on + strand at 1947.105 kb on - strand, within PS417_08945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,944,742 + PS417_08925 0.42 -0.2 1,944,816 - PS417_08925 0.52 -0.9 1,944,816 - PS417_08925 0.52 +0.1 1,944,851 + PS417_08925 0.57 +0.1 1,944,995 + PS417_08925 0.76 +0.4 1,944,995 + PS417_08925 0.76 -3.2 1,944,996 - PS417_08925 0.76 +1.9 1,945,121 + +0.3 1,945,121 + -0.4 1,945,122 - -2.3 1,945,122 - +0.1 1,945,169 + -0.7 1,945,173 + -0.3 1,945,174 - -0.1 1,945,217 + -3.5 1,945,245 + +2.3 1,945,245 + +0.2 1,945,245 + +1.7 1,945,373 - PS417_08930 0.44 -0.2 1,945,373 - PS417_08930 0.44 -0.6 1,945,499 + +0.8 1,945,499 + -0.9 1,945,499 + -0.2 1,945,500 - -0.4 1,945,500 - +0.2 1,945,537 - +0.3 1,945,537 - -1.3 1,945,540 + -1.3 1,945,540 + +0.3 1,945,572 - +0.1 1,945,588 + -0.4 1,945,589 - +0.7 1,945,592 + -2.9 1,945,592 + +1.2 1,945,592 + +0.7 1,945,592 + +1.1 1,945,593 - -1.1 1,945,593 - -1.3 1,945,593 - -1.4 1,945,593 - -3.1 1,945,593 - -0.6 1,945,594 + +0.6 1,945,594 + +0.5 1,945,594 + -1.3 1,945,595 - -1.1 1,945,595 - -0.9 1,945,597 - -3.2 1,945,597 - -0.0 1,945,652 + +0.5 1,945,655 + +0.3 1,945,775 + PS417_08935 0.22 +0.2 1,945,775 + PS417_08935 0.22 -0.7 1,945,792 - PS417_08935 0.25 -0.4 1,945,892 - PS417_08935 0.46 +1.6 1,945,892 - PS417_08935 0.46 -2.0 1,945,938 + PS417_08935 0.56 -0.2 1,945,938 + PS417_08935 0.56 +1.2 1,945,938 + PS417_08935 0.56 -1.1 1,945,939 - +0.4 1,946,031 + PS417_08935 0.75 -0.6 1,946,078 + PS417_08935 0.85 +2.3 1,946,078 + PS417_08935 0.85 -0.9 1,946,079 - PS417_08935 0.86 -0.4 1,946,079 - PS417_08935 0.86 -1.3 1,946,196 - PS417_08940 0.11 -0.9 1,946,299 + PS417_08940 0.34 -1.1 1,946,299 + PS417_08940 0.34 +1.2 1,946,300 - PS417_08940 0.34 -2.2 1,946,300 - PS417_08940 0.34 -1.1 1,946,300 - PS417_08940 0.34 +0.2 1,946,463 - +1.7 1,946,768 - +0.0 1,946,831 + -1.2 1,947,105 - PS417_08945 0.22 +1.5
Or see this region's nucleotide sequence