Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05915 and PS417_05920 are separated by 63 nucleotides PS417_05920 and PS417_05925 are separated by 177 nucleotides PS417_05925 and PS417_05930 overlap by 11 nucleotides
PS417_05915: PS417_05915 - spermidine dehydrogenase, at 1,313,856 to 1,315,718
_05915
PS417_05920: PS417_05920 - gamma-aminobutyraldehyde dehydrogenase, at 1,315,782 to 1,317,206
_05920
PS417_05925: PS417_05925 - spermidine/putrescine ABC transporter permease, at 1,317,384 to 1,318,193
_05925
PS417_05930: PS417_05930 - spermidine/putrescine ABC transporter permease, at 1,318,183 to 1,319,112
_05930
Position (kb)
1315
1316
1317
1318 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1314.895 kb on + strand, within PS417_05915 at 1314.921 kb on + strand, within PS417_05915 at 1314.964 kb on + strand, within PS417_05915 at 1314.964 kb on + strand, within PS417_05915 at 1314.964 kb on + strand, within PS417_05915 at 1315.001 kb on - strand, within PS417_05915 at 1315.248 kb on + strand, within PS417_05915 at 1315.249 kb on - strand, within PS417_05915 at 1315.249 kb on - strand, within PS417_05915 at 1315.249 kb on - strand, within PS417_05915 at 1315.250 kb on + strand, within PS417_05915 at 1315.250 kb on + strand, within PS417_05915 at 1315.251 kb on - strand, within PS417_05915 at 1315.251 kb on - strand, within PS417_05915 at 1315.294 kb on + strand, within PS417_05915 at 1315.295 kb on - strand, within PS417_05915 at 1315.295 kb on - strand, within PS417_05915 at 1315.498 kb on + strand, within PS417_05915 at 1315.499 kb on - strand, within PS417_05915 at 1315.615 kb on + strand at 1315.616 kb on - strand at 1315.712 kb on + strand at 1315.743 kb on + strand at 1315.744 kb on - strand at 1315.744 kb on - strand at 1315.744 kb on - strand at 1315.816 kb on + strand at 1315.832 kb on - strand at 1315.832 kb on - strand at 1315.832 kb on - strand at 1315.908 kb on - strand at 1315.962 kb on + strand, within PS417_05920 at 1316.281 kb on + strand, within PS417_05920 at 1316.329 kb on + strand, within PS417_05920 at 1316.330 kb on - strand, within PS417_05920 at 1316.330 kb on - strand, within PS417_05920 at 1316.386 kb on + strand, within PS417_05920 at 1316.386 kb on + strand, within PS417_05920 at 1316.387 kb on - strand, within PS417_05920 at 1316.706 kb on - strand, within PS417_05920 at 1316.758 kb on + strand, within PS417_05920 at 1316.758 kb on + strand, within PS417_05920 at 1316.759 kb on - strand, within PS417_05920 at 1316.830 kb on + strand, within PS417_05920 at 1316.831 kb on - strand, within PS417_05920 at 1316.886 kb on + strand, within PS417_05920 at 1316.887 kb on - strand, within PS417_05920 at 1316.887 kb on - strand, within PS417_05920 at 1317.144 kb on + strand at 1317.172 kb on - strand at 1317.221 kb on - strand at 1317.386 kb on - strand at 1317.430 kb on + strand at 1317.430 kb on + strand at 1317.434 kb on - strand at 1317.434 kb on - strand at 1317.434 kb on - strand at 1317.434 kb on - strand at 1317.656 kb on - strand, within PS417_05925 at 1317.749 kb on - strand, within PS417_05925 at 1317.759 kb on + strand, within PS417_05925 at 1317.923 kb on - strand, within PS417_05925 at 1318.102 kb on + strand, within PS417_05925 at 1318.172 kb on + strand at 1318.173 kb on - strand at 1318.173 kb on - strand at 1318.173 kb on - strand at 1318.173 kb on - strand at 1318.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,314,895 + PS417_05915 0.56 +0.2 1,314,921 + PS417_05915 0.57 +0.3 1,314,964 + PS417_05915 0.59 +1.1 1,314,964 + PS417_05915 0.59 -1.5 1,314,964 + PS417_05915 0.59 -0.3 1,315,001 - PS417_05915 0.61 -1.6 1,315,248 + PS417_05915 0.75 +0.8 1,315,249 - PS417_05915 0.75 -1.5 1,315,249 - PS417_05915 0.75 -0.6 1,315,249 - PS417_05915 0.75 -0.9 1,315,250 + PS417_05915 0.75 +0.3 1,315,250 + PS417_05915 0.75 -1.8 1,315,251 - PS417_05915 0.75 -0.2 1,315,251 - PS417_05915 0.75 +0.7 1,315,294 + PS417_05915 0.77 +0.9 1,315,295 - PS417_05915 0.77 +0.9 1,315,295 - PS417_05915 0.77 -0.1 1,315,498 + PS417_05915 0.88 +0.9 1,315,499 - PS417_05915 0.88 -0.0 1,315,615 + -0.3 1,315,616 - -0.7 1,315,712 + -0.2 1,315,743 + +2.2 1,315,744 - +1.2 1,315,744 - -1.1 1,315,744 - -1.5 1,315,816 + +0.4 1,315,832 - -0.2 1,315,832 - -0.5 1,315,832 - +0.2 1,315,908 - -0.7 1,315,962 + PS417_05920 0.13 -1.8 1,316,281 + PS417_05920 0.35 +0.0 1,316,329 + PS417_05920 0.38 -1.3 1,316,330 - PS417_05920 0.38 +1.1 1,316,330 - PS417_05920 0.38 +0.7 1,316,386 + PS417_05920 0.42 -1.6 1,316,386 + PS417_05920 0.42 +0.3 1,316,387 - PS417_05920 0.42 +0.0 1,316,706 - PS417_05920 0.65 -2.4 1,316,758 + PS417_05920 0.68 -1.1 1,316,758 + PS417_05920 0.68 +1.2 1,316,759 - PS417_05920 0.69 -1.3 1,316,830 + PS417_05920 0.74 +0.3 1,316,831 - PS417_05920 0.74 -0.6 1,316,886 + PS417_05920 0.77 +0.7 1,316,887 - PS417_05920 0.78 -1.5 1,316,887 - PS417_05920 0.78 +0.3 1,317,144 + +0.2 1,317,172 - -2.4 1,317,221 - -1.7 1,317,386 - +0.5 1,317,430 + -2.6 1,317,430 + -0.4 1,317,434 - +0.5 1,317,434 - -0.1 1,317,434 - -0.1 1,317,434 - -1.6 1,317,656 - PS417_05925 0.34 -0.3 1,317,749 - PS417_05925 0.45 -1.1 1,317,759 + PS417_05925 0.46 +0.3 1,317,923 - PS417_05925 0.67 +0.4 1,318,102 + PS417_05925 0.89 -2.0 1,318,172 + +0.1 1,318,173 - +0.6 1,318,173 - -1.7 1,318,173 - -0.9 1,318,173 - +0.3 1,318,173 - +0.5
Or see this region's nucleotide sequence