Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05150 and PS417_05155 are separated by 212 nucleotides PS417_05155 and PS417_05160 are separated by 7 nucleotides PS417_05160 and PS417_05165 are separated by 67 nucleotides
PS417_05150: PS417_05150 - valyl-tRNA synthetase, at 1,141,726 to 1,144,572
_05150
PS417_05155: PS417_05155 - DNA polymerase III subunit chi, at 1,144,785 to 1,145,168
_05155
PS417_05160: PS417_05160 - DNA polymerase III subunit chi, at 1,145,176 to 1,145,604
_05160
PS417_05165: PS417_05165 - cytosol aminopeptidase, at 1,145,672 to 1,147,162
_05165
Position (kb)
1145
1146 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1144.598 kb on - strand at 1144.619 kb on - strand at 1144.619 kb on - strand at 1144.666 kb on + strand at 1144.667 kb on - strand at 1144.667 kb on - strand at 1144.667 kb on - strand at 1144.779 kb on + strand at 1144.779 kb on + strand at 1144.795 kb on + strand at 1144.795 kb on + strand at 1145.043 kb on + strand, within PS417_05155 at 1145.044 kb on - strand, within PS417_05155 at 1145.189 kb on - strand at 1145.189 kb on - strand at 1145.498 kb on + strand, within PS417_05160 at 1145.498 kb on + strand, within PS417_05160 at 1145.581 kb on + strand at 1145.581 kb on + strand at 1145.581 kb on + strand at 1145.582 kb on - strand at 1145.582 kb on - strand at 1145.583 kb on + strand at 1145.583 kb on + strand at 1145.583 kb on + strand at 1145.583 kb on + strand at 1145.583 kb on + strand at 1145.583 kb on + strand at 1145.584 kb on - strand at 1145.584 kb on - strand at 1145.584 kb on - strand at 1145.584 kb on - strand at 1145.586 kb on - strand at 1145.586 kb on - strand at 1145.586 kb on - strand at 1145.586 kb on - strand at 1146.300 kb on + strand, within PS417_05165 at 1146.301 kb on - strand, within PS417_05165 at 1146.301 kb on - strand, within PS417_05165 at 1146.411 kb on + strand, within PS417_05165 at 1146.412 kb on - strand, within PS417_05165
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,144,598 - +1.2 1,144,619 - +0.1 1,144,619 - -1.0 1,144,666 + +1.2 1,144,667 - -0.0 1,144,667 - -0.3 1,144,667 - +0.7 1,144,779 + -0.0 1,144,779 + -0.3 1,144,795 + +0.1 1,144,795 + -0.1 1,145,043 + PS417_05155 0.67 +0.7 1,145,044 - PS417_05155 0.67 +0.5 1,145,189 - -0.4 1,145,189 - +0.7 1,145,498 + PS417_05160 0.75 -1.6 1,145,498 + PS417_05160 0.75 -1.2 1,145,581 + +0.8 1,145,581 + -0.0 1,145,581 + +0.3 1,145,582 - -1.0 1,145,582 - -0.3 1,145,583 + -0.8 1,145,583 + -0.2 1,145,583 + +0.9 1,145,583 + +0.2 1,145,583 + +0.5 1,145,583 + +0.1 1,145,584 - -0.2 1,145,584 - -0.4 1,145,584 - +0.7 1,145,584 - -0.7 1,145,586 - -2.1 1,145,586 - +0.4 1,145,586 - -1.8 1,145,586 - -0.6 1,146,300 + PS417_05165 0.42 +1.8 1,146,301 - PS417_05165 0.42 -0.3 1,146,301 - PS417_05165 0.42 -0.2 1,146,411 + PS417_05165 0.50 +0.7 1,146,412 - PS417_05165 0.50 +0.2
Or see this region's nucleotide sequence