Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00475 and PS417_00480 are separated by 103 nucleotides PS417_00480 and PS417_00485 overlap by 4 nucleotides PS417_00485 and PS417_00490 are separated by 1 nucleotides
PS417_00475: PS417_00475 - membrane protein, at 94,106 to 95,335
_00475
PS417_00480: PS417_00480 - hypothetical protein, at 95,439 to 96,050
_00480
PS417_00485: PS417_00485 - ABC transporter permease, at 96,047 to 96,985
_00485
PS417_00490: PS417_00490 - iron ABC transporter ATP-binding protein, at 96,987 to 97,790
_00490
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.725 kb on - strand, within PS417_00475 at 94.725 kb on - strand, within PS417_00475 at 94.886 kb on + strand, within PS417_00475 at 94.909 kb on + strand, within PS417_00475 at 95.207 kb on + strand, within PS417_00475 at 95.207 kb on + strand, within PS417_00475 at 95.207 kb on + strand, within PS417_00475 at 95.208 kb on - strand, within PS417_00475 at 95.208 kb on - strand, within PS417_00475 at 95.208 kb on - strand, within PS417_00475 at 95.223 kb on - strand at 95.318 kb on + strand at 95.319 kb on - strand at 95.374 kb on + strand at 95.375 kb on - strand at 95.375 kb on - strand at 95.698 kb on + strand, within PS417_00480 at 95.699 kb on - strand, within PS417_00480 at 95.717 kb on + strand, within PS417_00480 at 95.768 kb on + strand, within PS417_00480 at 95.821 kb on + strand, within PS417_00480 at 96.018 kb on + strand at 96.019 kb on - strand at 96.030 kb on + strand at 96.763 kb on + strand, within PS417_00485 at 96.867 kb on + strand, within PS417_00485 at 96.990 kb on + strand at 96.991 kb on - strand at 96.991 kb on - strand at 96.991 kb on - strand at 96.999 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 94,725 - PS417_00475 0.50 -3.3 94,725 - PS417_00475 0.50 +1.8 94,886 + PS417_00475 0.63 +1.6 94,909 + PS417_00475 0.65 -1.1 95,207 + PS417_00475 0.90 -3.1 95,207 + PS417_00475 0.90 -2.4 95,207 + PS417_00475 0.90 +0.8 95,208 - PS417_00475 0.90 -0.1 95,208 - PS417_00475 0.90 -0.2 95,208 - PS417_00475 0.90 +1.0 95,223 - +0.6 95,318 + +0.2 95,319 - +0.5 95,374 + +1.2 95,375 - -0.4 95,375 - +0.9 95,698 + PS417_00480 0.42 +1.1 95,699 - PS417_00480 0.42 +0.5 95,717 + PS417_00480 0.45 +0.8 95,768 + PS417_00480 0.54 -0.1 95,821 + PS417_00480 0.62 +2.4 96,018 + +1.0 96,019 - -0.2 96,030 + +1.3 96,763 + PS417_00485 0.76 +0.7 96,867 + PS417_00485 0.87 +0.4 96,990 + +1.0 96,991 - +0.3 96,991 - -0.1 96,991 - +0.4 96,999 + -0.3
Or see this region's nucleotide sequence