Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00465 and PS417_00470 are separated by 728 nucleotides PS417_00470 and PS417_00475 are separated by 98 nucleotides
PS417_00465: PS417_00465 - hypothetical protein, at 91,480 to 91,947
_00465
PS417_00470: PS417_00470 - glutamate:protein symporter, at 92,676 to 94,007
_00470
PS417_00475: PS417_00475 - membrane protein, at 94,106 to 95,335
_00475
Position (kb)
92
93
94
95 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 91.714 kb on + strand at 91.714 kb on + strand, within PS417_00465 at 91.714 kb on + strand at 91.715 kb on - strand, within PS417_00465 at 91.715 kb on - strand, within PS417_00465 at 91.801 kb on + strand, within PS417_00465 at 91.801 kb on + strand, within PS417_00465 at 91.802 kb on - strand, within PS417_00465 at 91.843 kb on - strand, within PS417_00465 at 91.935 kb on + strand at 91.936 kb on - strand at 91.936 kb on - strand at 91.942 kb on + strand at 91.942 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 92.090 kb on + strand at 92.090 kb on + strand at 92.185 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.208 kb on - strand at 92.208 kb on - strand at 92.254 kb on + strand at 92.254 kb on + strand at 92.258 kb on + strand at 92.259 kb on - strand at 92.279 kb on + strand at 92.279 kb on + strand at 92.280 kb on - strand at 92.531 kb on + strand at 92.531 kb on + strand at 92.557 kb on + strand at 92.648 kb on + strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.669 kb on + strand at 92.693 kb on - strand at 92.813 kb on + strand, within PS417_00470 at 92.814 kb on - strand, within PS417_00470 at 92.814 kb on - strand, within PS417_00470 at 92.935 kb on - strand, within PS417_00470 at 93.020 kb on + strand, within PS417_00470 at 93.049 kb on - strand, within PS417_00470 at 93.122 kb on + strand, within PS417_00470 at 93.289 kb on - strand, within PS417_00470 at 93.495 kb on + strand, within PS417_00470 at 93.628 kb on - strand, within PS417_00470 at 93.628 kb on - strand, within PS417_00470 at 93.663 kb on + strand, within PS417_00470 at 93.663 kb on + strand, within PS417_00470 at 93.664 kb on - strand, within PS417_00470 at 93.664 kb on - strand, within PS417_00470 at 93.731 kb on + strand, within PS417_00470 at 93.732 kb on - strand, within PS417_00470 at 93.915 kb on + strand at 93.915 kb on + strand at 93.915 kb on + strand at 93.915 kb on + strand at 93.915 kb on + strand at 93.916 kb on - strand at 93.916 kb on - strand at 93.916 kb on - strand at 94.072 kb on + strand at 94.725 kb on - strand, within PS417_00475 at 94.725 kb on - strand, within PS417_00475 at 94.886 kb on + strand, within PS417_00475 at 94.909 kb on + strand, within PS417_00475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 91,714 + +1.8 91,714 + PS417_00465 0.50 -1.1 91,714 + -1.0 91,715 - PS417_00465 0.50 -1.6 91,715 - PS417_00465 0.50 -1.2 91,801 + PS417_00465 0.69 +0.2 91,801 + PS417_00465 0.69 -0.7 91,802 - PS417_00465 0.69 +0.5 91,843 - PS417_00465 0.78 +0.0 91,935 + -0.1 91,936 - -0.3 91,936 - +0.2 91,942 + -0.2 91,942 + -2.3 91,945 + -0.7 91,945 + -0.9 91,945 + -0.3 91,945 + +0.2 91,945 + +0.5 91,946 - -0.3 91,946 - -0.4 91,946 - -0.3 91,946 - +1.8 91,946 - -0.2 92,090 + -0.4 92,090 + +0.8 92,185 + +0.4 92,207 + +0.4 92,207 + +1.6 92,207 + -0.1 92,207 + +0.8 92,208 - -0.7 92,208 - -2.5 92,254 + -0.4 92,254 + -0.5 92,258 + -3.4 92,259 - +0.5 92,279 + -0.6 92,279 + -0.6 92,280 - -2.1 92,531 + +1.8 92,531 + -0.0 92,557 + -1.4 92,648 + -0.1 92,663 + +0.2 92,663 + -1.7 92,663 + -2.0 92,669 + -0.2 92,693 - +0.7 92,813 + PS417_00470 0.10 +1.4 92,814 - PS417_00470 0.10 +1.7 92,814 - PS417_00470 0.10 -0.3 92,935 - PS417_00470 0.19 -0.9 93,020 + PS417_00470 0.26 +0.0 93,049 - PS417_00470 0.28 +0.9 93,122 + PS417_00470 0.33 +1.4 93,289 - PS417_00470 0.46 -3.1 93,495 + PS417_00470 0.61 -1.2 93,628 - PS417_00470 0.71 -1.2 93,628 - PS417_00470 0.71 -0.8 93,663 + PS417_00470 0.74 -3.1 93,663 + PS417_00470 0.74 +1.4 93,664 - PS417_00470 0.74 +0.3 93,664 - PS417_00470 0.74 +0.5 93,731 + PS417_00470 0.79 -1.7 93,732 - PS417_00470 0.79 +1.4 93,915 + +3.0 93,915 + -0.2 93,915 + +0.8 93,915 + +0.6 93,915 + -0.6 93,916 - +1.3 93,916 - -1.4 93,916 - +0.1 94,072 + +1.7 94,725 - PS417_00475 0.50 -3.3 94,725 - PS417_00475 0.50 +1.8 94,886 + PS417_00475 0.63 +1.6 94,909 + PS417_00475 0.65 -1.1
Or see this region's nucleotide sequence