Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02900 and PS417_02905 overlap by 8 nucleotides PS417_02905 and PS417_02910 overlap by 1 nucleotides PS417_02910 and PS417_02915 overlap by 1 nucleotides
PS417_02900: PS417_02900 - oxidoreductase, at 661,811 to 663,157
_02900
PS417_02905: PS417_02905 - precorrin isomerase, at 663,150 to 663,776
_02905
PS417_02910: PS417_02910 - precorrin-2 C20-methyltransferase, at 663,776 to 664,507
_02910
PS417_02915: PS417_02915 - precorrin-3B C17-methyltransferase, at 664,507 to 666,144
_02915
Position (kb) 663
664
665 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 663.044 kb on + strand at 663.101 kb on - strand at 663.160 kb on - strand at 664.008 kb on - strand, within PS417_02910 at 664.124 kb on + strand, within PS417_02910 at 664.125 kb on - strand, within PS417_02910 at 664.125 kb on - strand, within PS417_02910 at 664.130 kb on + strand, within PS417_02910 at 664.130 kb on + strand, within PS417_02910 at 664.229 kb on + strand, within PS417_02910 at 664.391 kb on + strand, within PS417_02910 at 664.392 kb on - strand, within PS417_02910 at 664.524 kb on + strand at 664.525 kb on - strand at 664.631 kb on - strand at 664.663 kb on + strand at 664.991 kb on + strand at 665.114 kb on - strand, within PS417_02915 at 665.482 kb on + strand, within PS417_02915 at 665.483 kb on - strand, within PS417_02915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 663,044 + +0.3 663,101 - -1.0 663,160 - +0.3 664,008 - PS417_02910 0.32 +2.1 664,124 + PS417_02910 0.48 -0.5 664,125 - PS417_02910 0.48 -0.1 664,125 - PS417_02910 0.48 +0.2 664,130 + PS417_02910 0.48 +0.9 664,130 + PS417_02910 0.48 +3.3 664,229 + PS417_02910 0.62 -0.4 664,391 + PS417_02910 0.84 -2.0 664,392 - PS417_02910 0.84 -1.1 664,524 + +0.4 664,525 - +0.0 664,631 - -1.5 664,663 + +0.9 664,991 + +0.1 665,114 - PS417_02915 0.37 -1.1 665,482 + PS417_02915 0.60 -1.1 665,483 - PS417_02915 0.60 -1.0
Or see this region's nucleotide sequence