Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28415 and PS417_28420 are separated by 22 nucleotides PS417_28420 and PS417_28425 are separated by 150 nucleotides PS417_28425 and PS417_28430 are separated by 77 nucleotides
PS417_28415: PS417_28415 - phosphatidate cytidylyltransferase, at 6,133,509 to 6,134,441
_28415
PS417_28420: PS417_28420 - AsnC family transcriptional regulator, at 6,134,464 to 6,134,937
_28420
PS417_28425: PS417_28425 - lysine transporter LysE, at 6,135,088 to 6,135,717
_28425
PS417_28430: PS417_28430 - alpha-2-macroglobulin, at 6,135,795 to 6,141,599
_28430
Position (kb)
6135
6136 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6134.092 kb on + strand, within PS417_28415 at 6134.092 kb on + strand, within PS417_28415 at 6134.092 kb on + strand, within PS417_28415 at 6134.093 kb on - strand, within PS417_28415 at 6134.100 kb on + strand, within PS417_28415 at 6134.100 kb on + strand, within PS417_28415 at 6134.101 kb on - strand, within PS417_28415 at 6134.101 kb on - strand, within PS417_28415 at 6134.226 kb on - strand, within PS417_28415 at 6134.345 kb on + strand, within PS417_28415 at 6134.346 kb on - strand, within PS417_28415 at 6134.448 kb on + strand at 6135.085 kb on + strand at 6135.085 kb on + strand at 6135.130 kb on + strand at 6135.342 kb on + strand, within PS417_28425 at 6135.363 kb on + strand, within PS417_28425 at 6135.363 kb on + strand, within PS417_28425 at 6135.364 kb on - strand, within PS417_28425 at 6135.586 kb on + strand, within PS417_28425 at 6135.646 kb on - strand, within PS417_28425 at 6135.761 kb on + strand at 6135.762 kb on - strand at 6135.762 kb on - strand at 6135.762 kb on - strand at 6135.762 kb on - strand at 6135.766 kb on + strand at 6135.967 kb on + strand at 6136.002 kb on + strand at 6136.002 kb on + strand at 6136.017 kb on + strand at 6136.050 kb on + strand at 6136.081 kb on - strand at 6136.272 kb on + strand at 6136.272 kb on + strand at 6136.272 kb on + strand at 6136.273 kb on - strand at 6136.319 kb on - strand at 6136.329 kb on + strand at 6136.329 kb on + strand at 6136.466 kb on - strand, within PS417_28430 at 6136.466 kb on - strand, within PS417_28430 at 6136.570 kb on - strand, within PS417_28430 at 6136.573 kb on + strand, within PS417_28430 at 6136.573 kb on + strand, within PS417_28430 at 6136.638 kb on - strand, within PS417_28430 at 6136.638 kb on - strand, within PS417_28430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 6,134,092 + PS417_28415 0.62 -1.4 6,134,092 + PS417_28415 0.62 -2.3 6,134,092 + PS417_28415 0.62 -0.0 6,134,093 - PS417_28415 0.63 -0.6 6,134,100 + PS417_28415 0.63 +0.3 6,134,100 + PS417_28415 0.63 +2.4 6,134,101 - PS417_28415 0.63 +0.4 6,134,101 - PS417_28415 0.63 +0.4 6,134,226 - PS417_28415 0.77 -0.1 6,134,345 + PS417_28415 0.90 -0.6 6,134,346 - PS417_28415 0.90 -0.0 6,134,448 + -0.0 6,135,085 + -1.4 6,135,085 + +2.5 6,135,130 + +1.1 6,135,342 + PS417_28425 0.40 -1.4 6,135,363 + PS417_28425 0.44 -0.7 6,135,363 + PS417_28425 0.44 -0.5 6,135,364 - PS417_28425 0.44 -0.0 6,135,586 + PS417_28425 0.79 +0.3 6,135,646 - PS417_28425 0.89 -1.2 6,135,761 + -0.9 6,135,762 - -0.3 6,135,762 - +0.8 6,135,762 - +2.5 6,135,762 - -0.4 6,135,766 + +0.2 6,135,967 + -0.1 6,136,002 + -1.1 6,136,002 + +0.2 6,136,017 + +3.4 6,136,050 + -2.2 6,136,081 - +1.5 6,136,272 + -1.8 6,136,272 + -0.6 6,136,272 + +2.8 6,136,273 - -2.1 6,136,319 - -0.6 6,136,329 + -0.2 6,136,329 + -0.4 6,136,466 - PS417_28430 0.12 -0.7 6,136,466 - PS417_28430 0.12 -0.2 6,136,570 - PS417_28430 0.13 -0.4 6,136,573 + PS417_28430 0.13 -0.2 6,136,573 + PS417_28430 0.13 -2.8 6,136,638 - PS417_28430 0.15 -0.6 6,136,638 - PS417_28430 0.15 -2.7
Or see this region's nucleotide sequence