Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28175 and PS417_28180 are separated by 10 nucleotides PS417_28180 and PS417_28185 are separated by 135 nucleotides PS417_28185 and PS417_28190 are separated by 112 nucleotides PS417_28190 and PS417_28195 overlap by 4 nucleotides
PS417_28175: PS417_28175 - GNAT family acetyltransferase, at 6,084,397 to 6,084,843
_28175
PS417_28180: PS417_28180 - GNAT family acetyltransferase, at 6,084,854 to 6,085,525
_28180
PS417_28185: PS417_28185 - homocysteine methyltransferase, at 6,085,661 to 6,086,557
_28185
PS417_28190: PS417_28190 - amino acid ABC transporter substrate-binding protein, at 6,086,670 to 6,087,491
_28190
PS417_28195: PS417_28195 - amino acid ABC transporter permease, at 6,087,488 to 6,088,318
_28195
Position (kb)
6085
6086
6087 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6085.013 kb on + strand, within PS417_28180 at 6085.013 kb on + strand, within PS417_28180 at 6085.014 kb on - strand, within PS417_28180 at 6085.091 kb on + strand, within PS417_28180 at 6085.129 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.256 kb on + strand, within PS417_28180 at 6085.256 kb on + strand, within PS417_28180 at 6085.257 kb on - strand, within PS417_28180 at 6085.257 kb on - strand, within PS417_28180 at 6085.322 kb on + strand, within PS417_28180 at 6085.322 kb on + strand, within PS417_28180 at 6085.523 kb on + strand at 6085.768 kb on - strand, within PS417_28185 at 6086.227 kb on - strand, within PS417_28185 at 6086.379 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.419 kb on - strand, within PS417_28185 at 6086.540 kb on + strand at 6086.540 kb on + strand at 6086.540 kb on + strand at 6086.567 kb on - strand at 6086.615 kb on + strand at 6087.016 kb on - strand, within PS417_28190 at 6087.130 kb on - strand, within PS417_28190 at 6087.137 kb on + strand, within PS417_28190 at 6087.181 kb on - strand, within PS417_28190 at 6087.211 kb on - strand, within PS417_28190 at 6087.348 kb on + strand, within PS417_28190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 6,085,013 + PS417_28180 0.24 -2.3 6,085,013 + PS417_28180 0.24 -1.4 6,085,014 - PS417_28180 0.24 -0.9 6,085,091 + PS417_28180 0.35 +0.1 6,085,129 - PS417_28180 0.41 +0.3 6,085,242 - PS417_28180 0.58 +2.5 6,085,242 - PS417_28180 0.58 -0.5 6,085,242 - PS417_28180 0.58 -0.3 6,085,256 + PS417_28180 0.60 -0.7 6,085,256 + PS417_28180 0.60 -1.1 6,085,257 - PS417_28180 0.60 +0.1 6,085,257 - PS417_28180 0.60 -0.7 6,085,322 + PS417_28180 0.70 +0.5 6,085,322 + PS417_28180 0.70 -1.5 6,085,523 + -0.2 6,085,768 - PS417_28185 0.12 +0.6 6,086,227 - PS417_28185 0.63 -1.0 6,086,379 + PS417_28185 0.80 -1.2 6,086,418 + PS417_28185 0.84 -1.8 6,086,418 + PS417_28185 0.84 -0.9 6,086,418 + PS417_28185 0.84 +0.2 6,086,419 - PS417_28185 0.85 -2.6 6,086,540 + -0.9 6,086,540 + +0.4 6,086,540 + +2.7 6,086,567 - -0.6 6,086,615 + +0.1 6,087,016 - PS417_28190 0.42 +1.4 6,087,130 - PS417_28190 0.56 +0.1 6,087,137 + PS417_28190 0.57 -0.2 6,087,181 - PS417_28190 0.62 +0.3 6,087,211 - PS417_28190 0.66 +1.2 6,087,348 + PS417_28190 0.82 -0.5
Or see this region's nucleotide sequence