Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27100 and PS417_27105 are separated by 123 nucleotides PS417_27105 and PS417_27110 overlap by 4 nucleotides PS417_27110 and PS417_27115 overlap by 4 nucleotides
PS417_27100: PS417_27100 - phosphohydrolase, at 5,850,258 to 5,850,899
_27100
PS417_27105: PS417_27105 - spermidine/putrescine ABC transporter permease, at 5,851,023 to 5,851,907
_27105
PS417_27110: PS417_27110 - spermidine/putrescine ABC transporter permease, at 5,851,904 to 5,852,824
_27110
PS417_27115: PS417_27115 - putrescine ABC transporter, ATPase component <small>(from data)</small>, at 5,852,821 to 5,853,963
_27115
Position (kb) 5851
5852 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5850.083 kb on + strand at 5850.084 kb on - strand at 5850.084 kb on - strand at 5850.084 kb on - strand at 5850.321 kb on + strand at 5850.322 kb on - strand at 5850.352 kb on - strand, within PS417_27100 at 5850.414 kb on + strand, within PS417_27100 at 5850.414 kb on + strand, within PS417_27100 at 5850.415 kb on - strand, within PS417_27100 at 5850.432 kb on + strand, within PS417_27100 at 5850.679 kb on - strand, within PS417_27100 at 5850.755 kb on + strand, within PS417_27100 at 5850.755 kb on + strand, within PS417_27100 at 5850.962 kb on + strand at 5850.978 kb on + strand at 5850.978 kb on + strand at 5850.978 kb on + strand at 5850.979 kb on - strand at 5850.979 kb on - strand at 5850.979 kb on - strand at 5850.985 kb on + strand at 5850.986 kb on - strand at 5850.986 kb on - strand at 5851.208 kb on + strand, within PS417_27105 at 5851.288 kb on - strand, within PS417_27105 at 5851.674 kb on - strand, within PS417_27105 at 5851.703 kb on - strand, within PS417_27105 at 5851.703 kb on - strand, within PS417_27105 at 5851.703 kb on - strand, within PS417_27105 at 5851.764 kb on + strand, within PS417_27105 at 5851.765 kb on - strand, within PS417_27105 at 5851.768 kb on + strand, within PS417_27105 at 5851.769 kb on - strand, within PS417_27105 at 5851.825 kb on + strand at 5851.853 kb on - strand at 5851.853 kb on - strand at 5851.856 kb on + strand at 5851.857 kb on - strand at 5851.907 kb on + strand at 5851.908 kb on - strand at 5852.280 kb on + strand, within PS417_27110 at 5852.281 kb on - strand, within PS417_27110 at 5852.281 kb on - strand, within PS417_27110 at 5852.281 kb on - strand, within PS417_27110 at 5852.295 kb on + strand, within PS417_27110 at 5852.296 kb on - strand, within PS417_27110 at 5852.296 kb on - strand, within PS417_27110 at 5852.297 kb on + strand, within PS417_27110 at 5852.403 kb on + strand, within PS417_27110 at 5852.601 kb on + strand, within PS417_27110 at 5852.833 kb on + strand at 5852.864 kb on + strand at 5852.864 kb on + strand at 5852.865 kb on - strand at 5852.865 kb on - strand at 5852.865 kb on - strand at 5852.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,850,083 + -0.5 5,850,084 - -0.6 5,850,084 - +1.2 5,850,084 - -2.8 5,850,321 + -0.7 5,850,322 - -0.5 5,850,352 - PS417_27100 0.15 +0.1 5,850,414 + PS417_27100 0.24 +0.2 5,850,414 + PS417_27100 0.24 -1.6 5,850,415 - PS417_27100 0.24 -0.3 5,850,432 + PS417_27100 0.27 -1.4 5,850,679 - PS417_27100 0.66 +0.8 5,850,755 + PS417_27100 0.77 -0.4 5,850,755 + PS417_27100 0.77 -0.6 5,850,962 + +1.2 5,850,978 + -0.1 5,850,978 + +0.2 5,850,978 + +0.2 5,850,979 - -0.6 5,850,979 - -0.5 5,850,979 - +1.6 5,850,985 + +0.9 5,850,986 - -0.2 5,850,986 - -0.3 5,851,208 + PS417_27105 0.21 -0.8 5,851,288 - PS417_27105 0.30 -1.1 5,851,674 - PS417_27105 0.74 -0.5 5,851,703 - PS417_27105 0.77 -1.0 5,851,703 - PS417_27105 0.77 -3.3 5,851,703 - PS417_27105 0.77 -0.9 5,851,764 + PS417_27105 0.84 -1.2 5,851,765 - PS417_27105 0.84 +0.5 5,851,768 + PS417_27105 0.84 -0.4 5,851,769 - PS417_27105 0.84 +0.2 5,851,825 + +0.4 5,851,853 - -1.4 5,851,853 - +4.1 5,851,856 + -1.0 5,851,857 - -0.7 5,851,907 + -1.2 5,851,908 - -0.0 5,852,280 + PS417_27110 0.41 -1.0 5,852,281 - PS417_27110 0.41 -2.2 5,852,281 - PS417_27110 0.41 -2.3 5,852,281 - PS417_27110 0.41 +0.1 5,852,295 + PS417_27110 0.42 +1.8 5,852,296 - PS417_27110 0.43 -2.6 5,852,296 - PS417_27110 0.43 +0.4 5,852,297 + PS417_27110 0.43 -2.1 5,852,403 + PS417_27110 0.54 -1.3 5,852,601 + PS417_27110 0.76 -1.0 5,852,833 + -0.1 5,852,864 + -0.9 5,852,864 + -0.6 5,852,865 - -0.5 5,852,865 - -1.6 5,852,865 - -1.4 5,852,865 - -2.5
Or see this region's nucleotide sequence