Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26315 and PS417_26320 are separated by 68 nucleotides PS417_26320 and PS417_26325 overlap by 4 nucleotides PS417_26325 and PS417_26330 are separated by 188 nucleotides
PS417_26315: PS417_26315 - glycine/betaine ABC transporter substrate-binding protein, at 5,687,222 to 5,688,169
_26315
PS417_26320: PS417_26320 - ABC transporter permease, at 5,688,238 to 5,689,083
_26320
PS417_26325: PS417_26325 - ABC transporter ATP-binding protein, at 5,689,080 to 5,690,258
_26325
PS417_26330: PS417_26330 - beta-aspartyl peptidase, at 5,690,447 to 5,692,438
_26330
Position (kb)
5689
5690
5691 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5688.120 kb on + strand at 5688.120 kb on + strand at 5688.120 kb on + strand at 5688.121 kb on - strand at 5688.121 kb on - strand at 5688.121 kb on - strand at 5688.121 kb on - strand at 5688.167 kb on + strand at 5688.234 kb on + strand at 5688.234 kb on + strand at 5688.235 kb on - strand at 5688.235 kb on - strand at 5688.264 kb on - strand at 5688.271 kb on + strand at 5688.271 kb on + strand at 5688.272 kb on - strand at 5688.272 kb on - strand at 5688.272 kb on - strand at 5688.316 kb on + strand at 5688.316 kb on + strand at 5688.317 kb on - strand at 5688.317 kb on - strand at 5688.439 kb on - strand, within PS417_26320 at 5688.439 kb on - strand, within PS417_26320 at 5688.439 kb on - strand, within PS417_26320 at 5688.604 kb on + strand, within PS417_26320 at 5688.604 kb on + strand, within PS417_26320 at 5688.605 kb on - strand, within PS417_26320 at 5688.715 kb on - strand, within PS417_26320 at 5688.755 kb on - strand, within PS417_26320 at 5689.063 kb on - strand at 5689.063 kb on - strand at 5689.068 kb on - strand at 5689.068 kb on - strand at 5689.824 kb on + strand, within PS417_26325 at 5689.825 kb on - strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.866 kb on + strand, within PS417_26325 at 5689.867 kb on - strand, within PS417_26325 at 5689.875 kb on - strand, within PS417_26325 at 5689.889 kb on + strand, within PS417_26325 at 5690.227 kb on - strand at 5690.227 kb on - strand at 5690.280 kb on + strand at 5690.280 kb on + strand at 5690.280 kb on + strand at 5690.281 kb on - strand at 5690.326 kb on + strand at 5690.326 kb on + strand at 5690.327 kb on - strand at 5690.386 kb on + strand at 5690.386 kb on + strand at 5690.387 kb on - strand at 5690.445 kb on + strand at 5690.446 kb on - strand at 5690.452 kb on + strand at 5690.453 kb on - strand at 5690.454 kb on + strand at 5690.454 kb on + strand at 5690.454 kb on + strand at 5690.454 kb on + strand at 5690.454 kb on + strand at 5690.455 kb on - strand at 5690.455 kb on - strand at 5690.566 kb on - strand at 5690.580 kb on + strand at 5690.581 kb on - strand at 5690.935 kb on + strand, within PS417_26330 at 5690.936 kb on - strand, within PS417_26330 at 5691.222 kb on + strand, within PS417_26330 at 5691.222 kb on + strand, within PS417_26330 at 5691.223 kb on - strand, within PS417_26330 at 5691.223 kb on - strand, within PS417_26330
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,688,120 + +1.9 5,688,120 + +0.5 5,688,120 + -0.8 5,688,121 - +3.3 5,688,121 - -0.9 5,688,121 - -0.5 5,688,121 - +1.0 5,688,167 + +1.7 5,688,234 + -1.4 5,688,234 + -2.4 5,688,235 - +0.6 5,688,235 - -0.7 5,688,264 - +0.9 5,688,271 + -0.4 5,688,271 + +0.0 5,688,272 - +0.1 5,688,272 - +0.0 5,688,272 - -0.3 5,688,316 + -0.4 5,688,316 + +0.4 5,688,317 - +0.1 5,688,317 - -0.2 5,688,439 - PS417_26320 0.24 -3.1 5,688,439 - PS417_26320 0.24 -0.3 5,688,439 - PS417_26320 0.24 +1.4 5,688,604 + PS417_26320 0.43 -0.9 5,688,604 + PS417_26320 0.43 -0.5 5,688,605 - PS417_26320 0.43 -0.1 5,688,715 - PS417_26320 0.56 +0.8 5,688,755 - PS417_26320 0.61 +0.5 5,689,063 - -1.1 5,689,063 - +4.8 5,689,068 - +0.0 5,689,068 - -0.3 5,689,824 + PS417_26325 0.63 -0.6 5,689,825 - PS417_26325 0.63 -0.7 5,689,866 + PS417_26325 0.67 +0.0 5,689,866 + PS417_26325 0.67 +0.7 5,689,866 + PS417_26325 0.67 +0.1 5,689,866 + PS417_26325 0.67 +0.1 5,689,866 + PS417_26325 0.67 -0.3 5,689,866 + PS417_26325 0.67 -0.3 5,689,866 + PS417_26325 0.67 -0.9 5,689,866 + PS417_26325 0.67 +0.6 5,689,867 - PS417_26325 0.67 -0.6 5,689,875 - PS417_26325 0.67 -1.4 5,689,889 + PS417_26325 0.69 -1.2 5,690,227 - -0.5 5,690,227 - +1.9 5,690,280 + -1.4 5,690,280 + +0.3 5,690,280 + -0.9 5,690,281 - -0.7 5,690,326 + -0.4 5,690,326 + -1.7 5,690,327 - -0.3 5,690,386 + -0.1 5,690,386 + -1.1 5,690,387 - -1.7 5,690,445 + +0.2 5,690,446 - +0.5 5,690,452 + +0.3 5,690,453 - -2.0 5,690,454 + -2.4 5,690,454 + -2.2 5,690,454 + -0.1 5,690,454 + -0.3 5,690,454 + -0.3 5,690,455 - +0.5 5,690,455 - +0.2 5,690,566 - +1.0 5,690,580 + +0.0 5,690,581 - -0.8 5,690,935 + PS417_26330 0.24 -0.2 5,690,936 - PS417_26330 0.25 +1.0 5,691,222 + PS417_26330 0.39 -0.7 5,691,222 + PS417_26330 0.39 +0.9 5,691,223 - PS417_26330 0.39 -0.6 5,691,223 - PS417_26330 0.39 -1.2
Or see this region's nucleotide sequence