Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02345 and PS417_02350 are separated by 120 nucleotides PS417_02350 and PS417_02355 are separated by 462 nucleotides
PS417_02345: PS417_02345 - 3-deoxy-D-manno-octulosonic acid transferase, at 535,078 to 536,355
_02345
PS417_02350: PS417_02350 - channel protein TolC, at 536,476 to 537,915
_02350
PS417_02355: PS417_02355 - thiamine biosynthesis protein ThiC, at 538,378 to 540,270
_02355
Position (kb) 536
537
538 Strain fitness (log2 ratio) -2
-1
0
1
2 at 536.401 kb on + strand at 536.401 kb on + strand at 536.402 kb on - strand at 536.465 kb on - strand at 536.467 kb on - strand at 536.467 kb on - strand at 536.467 kb on - strand at 536.477 kb on + strand at 536.478 kb on - strand at 536.480 kb on + strand at 536.555 kb on + strand at 536.645 kb on + strand, within PS417_02350 at 536.667 kb on - strand, within PS417_02350 at 536.667 kb on - strand, within PS417_02350 at 536.758 kb on + strand, within PS417_02350 at 536.759 kb on - strand, within PS417_02350 at 536.907 kb on - strand, within PS417_02350 at 536.907 kb on - strand, within PS417_02350 at 537.059 kb on + strand, within PS417_02350 at 537.060 kb on - strand, within PS417_02350 at 537.137 kb on + strand, within PS417_02350 at 537.137 kb on + strand, within PS417_02350 at 537.137 kb on + strand, within PS417_02350 at 537.255 kb on - strand, within PS417_02350 at 537.342 kb on - strand, within PS417_02350 at 537.810 kb on - strand at 537.966 kb on - strand at 538.012 kb on + strand at 538.012 kb on + strand at 538.013 kb on - strand at 538.102 kb on + strand at 538.102 kb on + strand at 538.103 kb on - strand at 538.349 kb on + strand at 538.579 kb on + strand at 538.597 kb on + strand, within PS417_02355 at 538.598 kb on - strand, within PS417_02355 at 538.667 kb on + strand, within PS417_02355 at 538.668 kb on - strand, within PS417_02355 at 538.813 kb on + strand, within PS417_02355 at 538.814 kb on - strand, within PS417_02355 at 538.814 kb on - strand, within PS417_02355 at 538.814 kb on - strand, within PS417_02355 at 538.850 kb on - strand, within PS417_02355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 536,401 + -0.4 536,401 + -1.3 536,402 - +0.2 536,465 - -2.0 536,467 - -0.0 536,467 - +2.4 536,467 - -0.6 536,477 + -1.2 536,478 - +1.3 536,480 + -1.1 536,555 + +0.2 536,645 + PS417_02350 0.12 -1.3 536,667 - PS417_02350 0.13 -0.0 536,667 - PS417_02350 0.13 -0.3 536,758 + PS417_02350 0.20 -1.3 536,759 - PS417_02350 0.20 +1.4 536,907 - PS417_02350 0.30 -0.8 536,907 - PS417_02350 0.30 -2.6 537,059 + PS417_02350 0.40 -0.3 537,060 - PS417_02350 0.41 +0.7 537,137 + PS417_02350 0.46 -0.0 537,137 + PS417_02350 0.46 +2.7 537,137 + PS417_02350 0.46 -0.3 537,255 - PS417_02350 0.54 -1.1 537,342 - PS417_02350 0.60 +0.3 537,810 - +0.0 537,966 - -0.5 538,012 + +0.4 538,012 + -0.6 538,013 - +0.5 538,102 + +0.2 538,102 + +0.5 538,103 - -0.9 538,349 + -0.0 538,579 + -0.3 538,597 + PS417_02355 0.12 -0.5 538,598 - PS417_02355 0.12 -0.3 538,667 + PS417_02355 0.15 -2.2 538,668 - PS417_02355 0.15 -0.2 538,813 + PS417_02355 0.23 -1.4 538,814 - PS417_02355 0.23 +0.3 538,814 - PS417_02355 0.23 -1.0 538,814 - PS417_02355 0.23 +2.5 538,850 - PS417_02355 0.25 +0.4
Or see this region's nucleotide sequence