Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23520 and PS417_23525 are separated by 54 nucleotides PS417_23525 and PS417_23530 overlap by 32 nucleotides
PS417_23520: PS417_23520 - cysteine biosynthesis protein CysZ, at 5,088,873 to 5,089,640
_23520
PS417_23525: PS417_23525 - glycoside hydrolase, at 5,089,695 to 5,090,897
_23525
PS417_23530: PS417_23530 - 1,2-oxophytodienoate reductase, at 5,090,866 to 5,091,969
_23530
Position (kb)
5089
5090
5091 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5088.736 kb on - strand at 5088.736 kb on - strand at 5088.751 kb on - strand at 5089.003 kb on - strand, within PS417_23520 at 5089.081 kb on - strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.193 kb on - strand, within PS417_23520 at 5089.193 kb on - strand, within PS417_23520 at 5089.639 kb on - strand at 5089.981 kb on - strand, within PS417_23525 at 5089.981 kb on - strand, within PS417_23525 at 5090.080 kb on - strand, within PS417_23525 at 5090.080 kb on - strand, within PS417_23525 at 5090.127 kb on + strand, within PS417_23525 at 5090.128 kb on - strand, within PS417_23525 at 5090.145 kb on - strand, within PS417_23525 at 5090.300 kb on - strand, within PS417_23525 at 5090.326 kb on - strand, within PS417_23525 at 5090.326 kb on - strand, within PS417_23525 at 5090.397 kb on + strand, within PS417_23525 at 5090.642 kb on + strand, within PS417_23525 at 5090.643 kb on - strand, within PS417_23525 at 5090.656 kb on - strand, within PS417_23525 at 5090.665 kb on + strand, within PS417_23525 at 5090.666 kb on - strand, within PS417_23525 at 5090.666 kb on - strand, within PS417_23525 at 5090.867 kb on + strand at 5090.867 kb on + strand at 5090.868 kb on - strand at 5090.971 kb on + strand at 5091.278 kb on + strand, within PS417_23530 at 5091.278 kb on + strand, within PS417_23530 at 5091.278 kb on + strand, within PS417_23530 at 5091.278 kb on + strand, within PS417_23530 at 5091.279 kb on - strand, within PS417_23530 at 5091.279 kb on - strand, within PS417_23530 at 5091.449 kb on + strand, within PS417_23530 at 5091.603 kb on - strand, within PS417_23530 at 5091.694 kb on + strand, within PS417_23530 at 5091.842 kb on + strand, within PS417_23530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,088,736 - +2.1 5,088,736 - +0.3 5,088,751 - +0.7 5,089,003 - PS417_23520 0.17 +0.1 5,089,081 - PS417_23520 0.27 -0.4 5,089,192 + PS417_23520 0.42 +0.1 5,089,192 + PS417_23520 0.42 -0.6 5,089,192 + PS417_23520 0.42 +1.0 5,089,192 + PS417_23520 0.42 +0.6 5,089,193 - PS417_23520 0.42 -0.5 5,089,193 - PS417_23520 0.42 +1.5 5,089,639 - -2.1 5,089,981 - PS417_23525 0.24 +0.5 5,089,981 - PS417_23525 0.24 -0.2 5,090,080 - PS417_23525 0.32 +1.2 5,090,080 - PS417_23525 0.32 -2.4 5,090,127 + PS417_23525 0.36 +1.2 5,090,128 - PS417_23525 0.36 -0.2 5,090,145 - PS417_23525 0.37 +0.7 5,090,300 - PS417_23525 0.50 -0.4 5,090,326 - PS417_23525 0.52 -1.5 5,090,326 - PS417_23525 0.52 -1.1 5,090,397 + PS417_23525 0.58 -0.0 5,090,642 + PS417_23525 0.79 +0.1 5,090,643 - PS417_23525 0.79 +0.5 5,090,656 - PS417_23525 0.80 +1.4 5,090,665 + PS417_23525 0.81 -0.1 5,090,666 - PS417_23525 0.81 +1.2 5,090,666 - PS417_23525 0.81 +1.4 5,090,867 + +1.1 5,090,867 + +1.4 5,090,868 - -0.6 5,090,971 + -0.3 5,091,278 + PS417_23530 0.37 -0.3 5,091,278 + PS417_23530 0.37 -1.5 5,091,278 + PS417_23530 0.37 +2.4 5,091,278 + PS417_23530 0.37 -0.7 5,091,279 - PS417_23530 0.37 -0.9 5,091,279 - PS417_23530 0.37 -0.3 5,091,449 + PS417_23530 0.53 +0.7 5,091,603 - PS417_23530 0.67 +1.2 5,091,694 + PS417_23530 0.75 -2.3 5,091,842 + PS417_23530 0.88 +1.9
Or see this region's nucleotide sequence