Strain Fitness in Pseudomonas simiae WCS417 around PS417_02090

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02080 and PS417_02085 overlap by 17 nucleotidesPS417_02085 and PS417_02090 overlap by 4 nucleotidesPS417_02090 and PS417_02095 overlap by 8 nucleotides PS417_02080: PS417_02080 - histidine ammonia-lyase, at 465,875 to 467,416 _02080 PS417_02085: PS417_02085 - glycosyl transferase, at 467,400 to 468,335 _02085 PS417_02090: PS417_02090 - glycosyl transferase, at 468,332 to 469,066 _02090 PS417_02095: PS417_02095 - AMP-binding protein, at 469,059 to 470,729 _02095 Position (kb) 468 469 470Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 467.512 kb on - strandat 468.556 kb on - strand, within PS417_02090at 468.678 kb on + strand, within PS417_02090at 468.679 kb on - strandat 469.130 kb on - strandat 469.130 kb on - strandat 469.209 kb on + strandat 469.262 kb on + strand, within PS417_02095at 469.328 kb on - strand, within PS417_02095at 469.825 kb on + strand, within PS417_02095at 469.826 kb on - strand, within PS417_02095

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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467,512 - +1.5
468,556 - PS417_02090 0.30 -0.2
468,678 + PS417_02090 0.47 +0.8
468,679 - +0.8
469,130 - +0.2
469,130 - -0.7
469,209 + -2.8
469,262 + PS417_02095 0.12 +0.4
469,328 - PS417_02095 0.16 +2.2
469,825 + PS417_02095 0.46 +1.6
469,826 - PS417_02095 0.46 +0.7

Or see this region's nucleotide sequence