Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20225 and PS417_20230 are separated by 313 nucleotides PS417_20230 and PS417_20235 are separated by 207 nucleotides PS417_20235 and PS417_20240 are separated by 65 nucleotides
PS417_20225: PS417_20225 - GntR family transcriptional regulator, at 4,393,946 to 4,394,782
_20225
PS417_20230: PS417_20230 - hypothetical protein, at 4,395,096 to 4,395,284
_20230
PS417_20235: PS417_20235 - hypothetical protein, at 4,395,492 to 4,396,214
_20235
PS417_20240: PS417_20240 - hypothetical protein, at 4,396,280 to 4,397,146
_20240
Position (kb)
4395
4396 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4394.203 kb on + strand, within PS417_20225 at 4394.350 kb on - strand, within PS417_20225 at 4394.350 kb on - strand, within PS417_20225 at 4394.424 kb on + strand, within PS417_20225 at 4394.442 kb on + strand, within PS417_20225 at 4394.527 kb on + strand, within PS417_20225 at 4394.550 kb on - strand, within PS417_20225 at 4394.662 kb on + strand, within PS417_20225 at 4394.663 kb on - strand, within PS417_20225 at 4394.675 kb on - strand, within PS417_20225 at 4394.676 kb on + strand, within PS417_20225 at 4394.737 kb on + strand at 4394.738 kb on - strand at 4394.738 kb on - strand at 4394.764 kb on - strand at 4394.789 kb on + strand at 4395.034 kb on + strand at 4395.112 kb on + strand at 4395.113 kb on - strand at 4395.282 kb on + strand at 4395.337 kb on + strand at 4395.509 kb on - strand at 4395.762 kb on + strand, within PS417_20235 at 4395.762 kb on + strand, within PS417_20235 at 4395.762 kb on + strand, within PS417_20235 at 4395.763 kb on - strand, within PS417_20235 at 4395.763 kb on - strand at 4395.763 kb on - strand, within PS417_20235 at 4395.763 kb on - strand, within PS417_20235 at 4395.880 kb on + strand, within PS417_20235 at 4395.880 kb on + strand, within PS417_20235 at 4395.881 kb on - strand, within PS417_20235 at 4395.994 kb on - strand, within PS417_20235 at 4396.047 kb on - strand, within PS417_20235 at 4396.111 kb on - strand, within PS417_20235 at 4396.149 kb on + strand at 4396.209 kb on + strand at 4396.209 kb on + strand at 4396.210 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,394,203 + PS417_20225 0.31 +0.4 4,394,350 - PS417_20225 0.48 +1.8 4,394,350 - PS417_20225 0.48 -0.3 4,394,424 + PS417_20225 0.57 -1.4 4,394,442 + PS417_20225 0.59 +1.0 4,394,527 + PS417_20225 0.69 -0.9 4,394,550 - PS417_20225 0.72 -0.5 4,394,662 + PS417_20225 0.86 +0.6 4,394,663 - PS417_20225 0.86 +0.3 4,394,675 - PS417_20225 0.87 -0.1 4,394,676 + PS417_20225 0.87 +1.1 4,394,737 + +1.1 4,394,738 - -0.0 4,394,738 - +1.2 4,394,764 - +1.0 4,394,789 + -1.9 4,395,034 + -0.3 4,395,112 + -1.6 4,395,113 - +0.1 4,395,282 + +0.1 4,395,337 + +0.7 4,395,509 - -2.9 4,395,762 + PS417_20235 0.37 -1.4 4,395,762 + PS417_20235 0.37 +0.1 4,395,762 + PS417_20235 0.37 -0.5 4,395,763 - PS417_20235 0.37 +0.2 4,395,763 - -2.8 4,395,763 - PS417_20235 0.37 +0.1 4,395,763 - PS417_20235 0.37 -0.3 4,395,880 + PS417_20235 0.54 -1.2 4,395,880 + PS417_20235 0.54 -0.2 4,395,881 - PS417_20235 0.54 +0.6 4,395,994 - PS417_20235 0.69 +0.2 4,396,047 - PS417_20235 0.77 +1.1 4,396,111 - PS417_20235 0.86 -1.7 4,396,149 + -2.3 4,396,209 + +0.6 4,396,209 + +0.3 4,396,210 - -0.2
Or see this region's nucleotide sequence