Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19265 and PS417_19270 are separated by 295 nucleotides PS417_19270 and PS417_19275 are separated by 68 nucleotides PS417_19275 and PS417_19280 are separated by 193 nucleotides PS417_19280 and PS417_19285 are separated by 22 nucleotides
PS417_19265: PS417_19265 - membrane protein, at 4,179,458 to 4,181,545
_19265
PS417_19270: PS417_19270 - hypothetical protein, at 4,181,841 to 4,182,032
_19270
PS417_19275: PS417_19275 - hypothetical protein, at 4,182,101 to 4,182,631
_19275
PS417_19280: PS417_19280 - hypothetical protein, at 4,182,825 to 4,183,418
_19280
PS417_19285: PS417_19285 - ankyrin, at 4,183,441 to 4,183,956
_19285
Position (kb)
4182
4183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4181.311 kb on - strand, within PS417_19265 at 4181.311 kb on - strand, within PS417_19265 at 4181.466 kb on + strand at 4181.571 kb on + strand at 4181.571 kb on + strand at 4181.571 kb on + strand at 4181.583 kb on + strand at 4181.583 kb on + strand at 4181.629 kb on + strand at 4181.630 kb on - strand at 4181.764 kb on + strand at 4181.764 kb on + strand at 4181.765 kb on - strand at 4181.774 kb on - strand at 4181.825 kb on + strand at 4181.825 kb on + strand at 4181.907 kb on + strand, within PS417_19270 at 4181.962 kb on - strand, within PS417_19270 at 4181.962 kb on - strand, within PS417_19270 at 4182.044 kb on + strand at 4182.065 kb on + strand at 4182.065 kb on + strand at 4182.066 kb on - strand at 4182.066 kb on - strand at 4182.244 kb on + strand, within PS417_19275 at 4182.256 kb on - strand, within PS417_19275 at 4182.689 kb on + strand at 4182.689 kb on + strand at 4182.690 kb on - strand at 4182.690 kb on - strand at 4182.690 kb on - strand at 4182.690 kb on - strand at 4182.765 kb on + strand at 4182.781 kb on - strand at 4182.781 kb on - strand at 4182.784 kb on + strand at 4182.784 kb on + strand at 4182.785 kb on - strand at 4182.785 kb on - strand at 4182.785 kb on - strand at 4182.785 kb on - strand at 4182.785 kb on - strand at 4182.785 kb on - strand at 4182.812 kb on - strand at 4182.838 kb on - strand at 4182.838 kb on - strand at 4182.838 kb on - strand at 4182.839 kb on + strand at 4182.849 kb on + strand at 4182.849 kb on + strand at 4182.850 kb on - strand at 4182.850 kb on - strand at 4182.977 kb on - strand, within PS417_19280 at 4182.991 kb on - strand, within PS417_19280 at 4183.045 kb on + strand, within PS417_19280 at 4183.046 kb on - strand, within PS417_19280 at 4183.099 kb on - strand, within PS417_19280 at 4183.125 kb on + strand, within PS417_19280 at 4183.141 kb on + strand, within PS417_19280 at 4183.184 kb on - strand, within PS417_19280 at 4183.276 kb on - strand, within PS417_19280 at 4183.323 kb on - strand, within PS417_19280 at 4183.350 kb on + strand, within PS417_19280 at 4183.351 kb on - strand, within PS417_19280 at 4183.384 kb on + strand at 4183.395 kb on + strand at 4183.395 kb on + strand at 4183.395 kb on + strand at 4183.395 kb on + strand at 4183.396 kb on - strand at 4183.396 kb on - strand at 4183.396 kb on - strand at 4183.396 kb on - strand at 4183.412 kb on - strand at 4183.416 kb on + strand at 4183.442 kb on + strand at 4183.559 kb on + strand, within PS417_19285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,181,311 - PS417_19265 0.89 +1.9 4,181,311 - PS417_19265 0.89 +2.5 4,181,466 + +1.8 4,181,571 + +1.2 4,181,571 + +2.3 4,181,571 + +2.4 4,181,583 + +1.4 4,181,583 + -1.0 4,181,629 + +2.6 4,181,630 - -0.5 4,181,764 + +0.5 4,181,764 + +0.8 4,181,765 - -2.0 4,181,774 - -0.6 4,181,825 + -0.7 4,181,825 + +1.0 4,181,907 + PS417_19270 0.34 -0.9 4,181,962 - PS417_19270 0.63 +0.1 4,181,962 - PS417_19270 0.63 +0.1 4,182,044 + -0.3 4,182,065 + +0.6 4,182,065 + +0.1 4,182,066 - +0.7 4,182,066 - -0.5 4,182,244 + PS417_19275 0.27 +0.1 4,182,256 - PS417_19275 0.29 -1.4 4,182,689 + -0.5 4,182,689 + -0.7 4,182,690 - -0.5 4,182,690 - +1.3 4,182,690 - -0.4 4,182,690 - +1.2 4,182,765 + +0.4 4,182,781 - +0.8 4,182,781 - +0.5 4,182,784 + -1.4 4,182,784 + -0.2 4,182,785 - +2.1 4,182,785 - +0.6 4,182,785 - +0.1 4,182,785 - +1.3 4,182,785 - -1.7 4,182,785 - -1.0 4,182,812 - +1.3 4,182,838 - -0.1 4,182,838 - -0.9 4,182,838 - -1.0 4,182,839 + +0.3 4,182,849 + -1.0 4,182,849 + +1.2 4,182,850 - +0.2 4,182,850 - +0.0 4,182,977 - PS417_19280 0.26 +0.1 4,182,991 - PS417_19280 0.28 +0.0 4,183,045 + PS417_19280 0.37 +2.6 4,183,046 - PS417_19280 0.37 -0.8 4,183,099 - PS417_19280 0.46 -0.2 4,183,125 + PS417_19280 0.51 -0.2 4,183,141 + PS417_19280 0.53 -1.8 4,183,184 - PS417_19280 0.60 -0.0 4,183,276 - PS417_19280 0.76 +1.6 4,183,323 - PS417_19280 0.84 -1.0 4,183,350 + PS417_19280 0.88 +0.2 4,183,351 - PS417_19280 0.89 -1.4 4,183,384 + -0.6 4,183,395 + -0.2 4,183,395 + +0.2 4,183,395 + +0.5 4,183,395 + +0.1 4,183,396 - -0.8 4,183,396 - -0.6 4,183,396 - -1.4 4,183,396 - -1.4 4,183,412 - +0.0 4,183,416 + -0.3 4,183,442 + +0.7 4,183,559 + PS417_19285 0.23 +2.0
Or see this region's nucleotide sequence