Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19225 and PS417_19230 are separated by 134 nucleotides PS417_19230 and PS417_19235 are separated by 3 nucleotides
PS417_19225: PS417_19225 - FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 4,170,615 to 4,172,615
_19225
PS417_19230: PS417_19230 - asparagine synthase, at 4,172,750 to 4,174,522
_19230
PS417_19235: PS417_19235 - GNAT family acetyltransferase, at 4,174,526 to 4,176,259
_19235
Position (kb)
4172
4173
4174
4175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4171.867 kb on + strand, within PS417_19225 at 4172.100 kb on + strand, within PS417_19225 at 4172.100 kb on + strand, within PS417_19225 at 4172.101 kb on - strand, within PS417_19225 at 4172.101 kb on - strand, within PS417_19225 at 4172.310 kb on + strand, within PS417_19225 at 4172.361 kb on + strand, within PS417_19225 at 4172.362 kb on - strand, within PS417_19225 at 4172.375 kb on + strand, within PS417_19225 at 4172.427 kb on + strand at 4172.428 kb on - strand at 4172.578 kb on + strand at 4172.645 kb on - strand at 4172.667 kb on - strand at 4172.750 kb on - strand at 4172.760 kb on + strand at 4172.985 kb on - strand, within PS417_19230 at 4172.985 kb on - strand, within PS417_19230 at 4172.985 kb on - strand, within PS417_19230 at 4173.036 kb on - strand, within PS417_19230 at 4173.192 kb on - strand, within PS417_19230 at 4173.192 kb on - strand, within PS417_19230 at 4173.260 kb on - strand, within PS417_19230 at 4173.296 kb on + strand, within PS417_19230 at 4173.296 kb on + strand, within PS417_19230 at 4173.332 kb on - strand, within PS417_19230 at 4173.696 kb on - strand, within PS417_19230 at 4173.717 kb on + strand, within PS417_19230 at 4173.718 kb on - strand, within PS417_19230 at 4173.810 kb on - strand, within PS417_19230 at 4173.810 kb on - strand, within PS417_19230 at 4174.108 kb on - strand, within PS417_19230 at 4174.548 kb on - strand at 4174.548 kb on - strand at 4174.583 kb on + strand at 4174.583 kb on + strand at 4174.681 kb on + strand at 4174.682 kb on - strand at 4174.754 kb on + strand, within PS417_19235 at 4174.776 kb on + strand, within PS417_19235 at 4174.800 kb on + strand, within PS417_19235 at 4174.928 kb on + strand, within PS417_19235 at 4174.928 kb on + strand, within PS417_19235 at 4175.043 kb on + strand at 4175.195 kb on + strand, within PS417_19235 at 4175.214 kb on + strand, within PS417_19235 at 4175.351 kb on + strand, within PS417_19235 at 4175.398 kb on - strand, within PS417_19235 at 4175.425 kb on - strand, within PS417_19235 at 4175.428 kb on + strand, within PS417_19235 at 4175.429 kb on - strand, within PS417_19235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,171,867 + PS417_19225 0.63 -1.3 4,172,100 + PS417_19225 0.74 -1.0 4,172,100 + PS417_19225 0.74 +1.6 4,172,101 - PS417_19225 0.74 +1.3 4,172,101 - PS417_19225 0.74 +1.7 4,172,310 + PS417_19225 0.85 -0.3 4,172,361 + PS417_19225 0.87 -0.5 4,172,362 - PS417_19225 0.87 -1.2 4,172,375 + PS417_19225 0.88 +0.6 4,172,427 + -1.7 4,172,428 - -0.8 4,172,578 + -1.0 4,172,645 - -3.3 4,172,667 - +0.5 4,172,750 - +0.8 4,172,760 + -1.0 4,172,985 - PS417_19230 0.13 +1.0 4,172,985 - PS417_19230 0.13 +0.1 4,172,985 - PS417_19230 0.13 +0.3 4,173,036 - PS417_19230 0.16 -1.1 4,173,192 - PS417_19230 0.25 -0.3 4,173,192 - PS417_19230 0.25 -1.2 4,173,260 - PS417_19230 0.29 -2.6 4,173,296 + PS417_19230 0.31 +0.3 4,173,296 + PS417_19230 0.31 +4.2 4,173,332 - PS417_19230 0.33 +0.8 4,173,696 - PS417_19230 0.53 -0.4 4,173,717 + PS417_19230 0.55 +0.7 4,173,718 - PS417_19230 0.55 +0.5 4,173,810 - PS417_19230 0.60 +1.1 4,173,810 - PS417_19230 0.60 -1.0 4,174,108 - PS417_19230 0.77 +0.8 4,174,548 - +1.6 4,174,548 - -1.2 4,174,583 + +0.6 4,174,583 + -0.7 4,174,681 + -1.0 4,174,682 - -0.4 4,174,754 + PS417_19235 0.13 +0.8 4,174,776 + PS417_19235 0.14 +0.0 4,174,800 + PS417_19235 0.16 +0.3 4,174,928 + PS417_19235 0.23 -0.3 4,174,928 + PS417_19235 0.23 +1.8 4,175,043 + -0.4 4,175,195 + PS417_19235 0.39 -0.3 4,175,214 + PS417_19235 0.40 +0.4 4,175,351 + PS417_19235 0.48 -1.4 4,175,398 - PS417_19235 0.50 +0.4 4,175,425 - PS417_19235 0.52 -1.0 4,175,428 + PS417_19235 0.52 +1.4 4,175,429 - PS417_19235 0.52 -0.8
Or see this region's nucleotide sequence