Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18020 and PS417_18025 are separated by 231 nucleotides PS417_18025 and PS417_18035 are separated by 167 nucleotides PS417_18035 and PS417_18040 are separated by 5 nucleotides PS417_18040 and PS417_18045 overlap by 4 nucleotides
PS417_18020: PS417_18020 - hypothetical protein, at 3,929,142 to 3,929,534
_18020
PS417_18025: PS417_18025 - cytochrome C, at 3,929,766 to 3,930,143
_18025
PS417_18035: PS417_18035 - general secretion pathway protein GspD, at 3,930,311 to 3,932,422
_18035
PS417_18040: PS417_18040 - general secretion pathway protein GspN, at 3,932,428 to 3,932,946
_18040
PS417_18045: PS417_18045 - general secretion pathway protein GspM, at 3,932,943 to 3,933,545
_18045
Position (kb)
3930
3931
3932
3933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3929.547 kb on - strand at 3929.623 kb on + strand at 3929.623 kb on + strand at 3929.643 kb on - strand at 3929.710 kb on - strand at 3929.722 kb on - strand at 3929.728 kb on + strand at 3929.857 kb on - strand, within PS417_18025 at 3929.858 kb on + strand, within PS417_18025 at 3929.859 kb on - strand, within PS417_18025 at 3929.859 kb on - strand, within PS417_18025 at 3930.007 kb on + strand, within PS417_18025 at 3930.008 kb on - strand, within PS417_18025 at 3930.008 kb on - strand at 3930.059 kb on - strand, within PS417_18025 at 3930.059 kb on - strand, within PS417_18025 at 3930.111 kb on + strand at 3930.302 kb on + strand at 3930.306 kb on + strand at 3930.306 kb on + strand at 3930.306 kb on + strand at 3930.307 kb on - strand at 3930.307 kb on - strand at 3930.307 kb on - strand at 3930.307 kb on - strand at 3930.307 kb on - strand at 3930.307 kb on - strand at 3930.307 kb on - strand at 3930.312 kb on + strand at 3930.312 kb on + strand at 3930.312 kb on + strand at 3930.313 kb on - strand at 3930.394 kb on + strand at 3930.394 kb on + strand at 3930.401 kb on - strand at 3930.483 kb on + strand at 3930.622 kb on + strand, within PS417_18035 at 3930.623 kb on - strand, within PS417_18035 at 3930.666 kb on + strand, within PS417_18035 at 3930.666 kb on + strand, within PS417_18035 at 3930.666 kb on + strand, within PS417_18035 at 3930.666 kb on + strand, within PS417_18035 at 3930.667 kb on - strand, within PS417_18035 at 3930.726 kb on + strand, within PS417_18035 at 3930.726 kb on + strand, within PS417_18035 at 3930.922 kb on - strand, within PS417_18035 at 3931.383 kb on + strand, within PS417_18035 at 3931.398 kb on + strand, within PS417_18035 at 3931.443 kb on + strand, within PS417_18035 at 3931.443 kb on + strand at 3931.444 kb on - strand, within PS417_18035 at 3931.444 kb on - strand, within PS417_18035 at 3931.444 kb on - strand, within PS417_18035 at 3931.720 kb on - strand, within PS417_18035 at 3931.720 kb on - strand, within PS417_18035 at 3931.843 kb on - strand, within PS417_18035 at 3931.947 kb on + strand, within PS417_18035 at 3932.083 kb on - strand, within PS417_18035 at 3932.454 kb on + strand at 3932.894 kb on - strand, within PS417_18040 at 3933.382 kb on + strand, within PS417_18045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,929,547 - -1.3 3,929,623 + +0.5 3,929,623 + +0.0 3,929,643 - +1.4 3,929,710 - -0.2 3,929,722 - +3.2 3,929,728 + -0.4 3,929,857 - PS417_18025 0.24 +3.4 3,929,858 + PS417_18025 0.24 -3.0 3,929,859 - PS417_18025 0.25 -1.2 3,929,859 - PS417_18025 0.25 -1.1 3,930,007 + PS417_18025 0.64 +0.2 3,930,008 - PS417_18025 0.64 -0.2 3,930,008 - -0.6 3,930,059 - PS417_18025 0.78 +0.8 3,930,059 - PS417_18025 0.78 +2.1 3,930,111 + +0.2 3,930,302 + -0.6 3,930,306 + +3.0 3,930,306 + -1.3 3,930,306 + -1.5 3,930,307 - -0.1 3,930,307 - -1.3 3,930,307 - +1.4 3,930,307 - -0.2 3,930,307 - +1.3 3,930,307 - +0.7 3,930,307 - +0.1 3,930,312 + -1.0 3,930,312 + -0.6 3,930,312 + -1.3 3,930,313 - +0.4 3,930,394 + +0.2 3,930,394 + -0.1 3,930,401 - -0.6 3,930,483 + -1.2 3,930,622 + PS417_18035 0.15 +0.7 3,930,623 - PS417_18035 0.15 +1.5 3,930,666 + PS417_18035 0.17 +3.5 3,930,666 + PS417_18035 0.17 +0.1 3,930,666 + PS417_18035 0.17 +1.2 3,930,666 + PS417_18035 0.17 -0.3 3,930,667 - PS417_18035 0.17 -1.1 3,930,726 + PS417_18035 0.20 +0.5 3,930,726 + PS417_18035 0.20 -0.2 3,930,922 - PS417_18035 0.29 -0.3 3,931,383 + PS417_18035 0.51 -0.3 3,931,398 + PS417_18035 0.51 +1.3 3,931,443 + PS417_18035 0.54 +0.9 3,931,443 + +1.7 3,931,444 - PS417_18035 0.54 +0.2 3,931,444 - PS417_18035 0.54 -1.9 3,931,444 - PS417_18035 0.54 -0.0 3,931,720 - PS417_18035 0.67 +1.0 3,931,720 - PS417_18035 0.67 +0.6 3,931,843 - PS417_18035 0.73 +0.1 3,931,947 + PS417_18035 0.77 +3.7 3,932,083 - PS417_18035 0.84 +0.3 3,932,454 + -0.1 3,932,894 - PS417_18040 0.90 +0.5 3,933,382 + PS417_18045 0.73 +1.3
Or see this region's nucleotide sequence