Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17155 and PS417_17160 overlap by 11 nucleotides PS417_17160 and PS417_17165 are separated by 57 nucleotides PS417_17165 and PS417_17170 are separated by 214 nucleotides
PS417_17155: PS417_17155 - cytochrome B561, at 3,745,998 to 3,746,555
_17155
PS417_17160: PS417_17160 - 1-aminocyclopropane-1-carboxylate deaminase, at 3,746,545 to 3,747,429
_17160
PS417_17165: PS417_17165 - 2,4-dienoyl-CoA reductase, at 3,747,487 to 3,749,526
_17165
PS417_17170: PS417_17170 - carbon-nitrogen hydrolase, at 3,749,741 to 3,750,871
_17170
Position (kb)
3747
3748
3749
3750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3746.666 kb on + strand, within PS417_17160 at 3746.667 kb on - strand, within PS417_17160 at 3746.785 kb on + strand, within PS417_17160 at 3747.722 kb on + strand, within PS417_17165 at 3748.061 kb on + strand, within PS417_17165 at 3748.062 kb on - strand, within PS417_17165 at 3748.062 kb on - strand, within PS417_17165 at 3748.227 kb on - strand, within PS417_17165 at 3748.415 kb on + strand, within PS417_17165 at 3748.782 kb on + strand, within PS417_17165 at 3748.881 kb on + strand, within PS417_17165 at 3748.881 kb on + strand, within PS417_17165 at 3748.882 kb on - strand, within PS417_17165 at 3748.959 kb on + strand, within PS417_17165 at 3749.018 kb on + strand, within PS417_17165 at 3749.052 kb on - strand, within PS417_17165 at 3749.312 kb on + strand at 3749.312 kb on + strand, within PS417_17165 at 3749.313 kb on - strand, within PS417_17165 at 3749.313 kb on - strand, within PS417_17165 at 3749.313 kb on - strand, within PS417_17165 at 3749.588 kb on + strand at 3749.589 kb on - strand at 3749.602 kb on + strand at 3749.602 kb on + strand at 3749.645 kb on + strand at 3749.646 kb on - strand at 3749.700 kb on + strand at 3749.701 kb on - strand at 3749.834 kb on + strand at 3749.834 kb on + strand at 3749.834 kb on + strand at 3749.834 kb on + strand at 3749.851 kb on + strand at 3749.851 kb on + strand at 3750.080 kb on + strand, within PS417_17170 at 3750.081 kb on - strand, within PS417_17170 at 3750.192 kb on - strand, within PS417_17170 at 3750.192 kb on - strand, within PS417_17170 at 3750.227 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.348 kb on - strand, within PS417_17170 at 3750.348 kb on - strand, within PS417_17170 at 3750.348 kb on - strand at 3750.350 kb on - strand, within PS417_17170 at 3750.419 kb on + strand, within PS417_17170 at 3750.420 kb on - strand, within PS417_17170 at 3750.436 kb on + strand, within PS417_17170 at 3750.498 kb on + strand, within PS417_17170 at 3750.498 kb on + strand, within PS417_17170 at 3750.499 kb on - strand, within PS417_17170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,746,666 + PS417_17160 0.14 +2.5 3,746,667 - PS417_17160 0.14 -1.0 3,746,785 + PS417_17160 0.27 -2.4 3,747,722 + PS417_17165 0.12 -1.0 3,748,061 + PS417_17165 0.28 -0.3 3,748,062 - PS417_17165 0.28 -1.4 3,748,062 - PS417_17165 0.28 -0.2 3,748,227 - PS417_17165 0.36 -2.7 3,748,415 + PS417_17165 0.45 -0.1 3,748,782 + PS417_17165 0.63 -0.2 3,748,881 + PS417_17165 0.68 -0.3 3,748,881 + PS417_17165 0.68 +0.2 3,748,882 - PS417_17165 0.68 -0.1 3,748,959 + PS417_17165 0.72 -0.5 3,749,018 + PS417_17165 0.75 +0.7 3,749,052 - PS417_17165 0.77 -1.0 3,749,312 + +3.1 3,749,312 + PS417_17165 0.89 -3.2 3,749,313 - PS417_17165 0.90 -2.2 3,749,313 - PS417_17165 0.90 -0.8 3,749,313 - PS417_17165 0.90 -0.0 3,749,588 + +2.5 3,749,589 - -0.3 3,749,602 + +0.2 3,749,602 + +1.4 3,749,645 + +2.0 3,749,646 - +0.0 3,749,700 + -0.4 3,749,701 - -0.5 3,749,834 + -0.6 3,749,834 + -2.3 3,749,834 + -0.6 3,749,834 + -1.1 3,749,851 + -1.1 3,749,851 + -1.4 3,750,080 + PS417_17170 0.30 -1.0 3,750,081 - PS417_17170 0.30 -0.3 3,750,192 - PS417_17170 0.40 -1.2 3,750,192 - PS417_17170 0.40 -0.5 3,750,227 + PS417_17170 0.43 -1.0 3,750,347 + PS417_17170 0.54 +0.3 3,750,347 + PS417_17170 0.54 -2.6 3,750,347 + PS417_17170 0.54 -1.2 3,750,347 + PS417_17170 0.54 -1.1 3,750,347 + PS417_17170 0.54 -0.8 3,750,347 + PS417_17170 0.54 +0.3 3,750,348 - PS417_17170 0.54 -0.7 3,750,348 - PS417_17170 0.54 -0.3 3,750,348 - +0.6 3,750,350 - PS417_17170 0.54 -1.8 3,750,419 + PS417_17170 0.60 -3.2 3,750,420 - PS417_17170 0.60 -0.4 3,750,436 + PS417_17170 0.61 -0.2 3,750,498 + PS417_17170 0.67 -1.6 3,750,498 + PS417_17170 0.67 -1.3 3,750,499 - PS417_17170 0.67 -1.1
Or see this region's nucleotide sequence