Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17070 and PS417_17075 overlap by 4 nucleotides PS417_17075 and PS417_17080 overlap by 4 nucleotides PS417_17080 and PS417_17085 overlap by 10 nucleotides
PS417_17070: PS417_17070 - RNA polymerase subunit sigma-24, at 3,729,380 to 3,729,886
_17070
PS417_17075: PS417_17075 - transmembrane anti-sigma factor, at 3,729,883 to 3,730,620
_17075
PS417_17080: PS417_17080 - lipoprotein, at 3,730,617 to 3,731,597
_17080
PS417_17085: PS417_17085 - fimbrial assembly protein, at 3,731,588 to 3,733,948
_17085
Position (kb)
3730
3731
3732 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3729.661 kb on + strand, within PS417_17070 at 3729.663 kb on - strand, within PS417_17070 at 3729.755 kb on + strand, within PS417_17070 at 3729.755 kb on + strand, within PS417_17070 at 3729.755 kb on + strand, within PS417_17070 at 3730.564 kb on - strand at 3730.568 kb on - strand at 3730.651 kb on + strand at 3730.685 kb on - strand at 3730.698 kb on - strand at 3730.699 kb on + strand at 3730.699 kb on + strand at 3730.700 kb on - strand at 3730.700 kb on - strand at 3730.823 kb on - strand, within PS417_17080 at 3730.899 kb on + strand, within PS417_17080 at 3730.899 kb on + strand, within PS417_17080 at 3730.899 kb on + strand, within PS417_17080 at 3730.899 kb on + strand, within PS417_17080 at 3731.082 kb on + strand at 3731.083 kb on - strand, within PS417_17080 at 3731.152 kb on + strand, within PS417_17080 at 3731.152 kb on + strand, within PS417_17080 at 3731.153 kb on - strand, within PS417_17080 at 3731.153 kb on - strand, within PS417_17080 at 3731.153 kb on - strand, within PS417_17080 at 3731.166 kb on + strand, within PS417_17080 at 3731.183 kb on + strand, within PS417_17080 at 3731.183 kb on + strand, within PS417_17080 at 3731.184 kb on - strand, within PS417_17080 at 3731.195 kb on - strand, within PS417_17080 at 3731.307 kb on - strand, within PS417_17080 at 3731.322 kb on - strand, within PS417_17080 at 3731.322 kb on - strand, within PS417_17080 at 3731.322 kb on - strand, within PS417_17080 at 3731.383 kb on + strand, within PS417_17080 at 3731.384 kb on - strand, within PS417_17080 at 3732.058 kb on + strand, within PS417_17085 at 3732.059 kb on - strand, within PS417_17085 at 3732.059 kb on - strand at 3732.059 kb on - strand, within PS417_17085 at 3732.265 kb on - strand, within PS417_17085 at 3732.300 kb on + strand, within PS417_17085 at 3732.300 kb on + strand, within PS417_17085 at 3732.300 kb on + strand, within PS417_17085 at 3732.301 kb on - strand, within PS417_17085 at 3732.530 kb on + strand, within PS417_17085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,729,661 + PS417_17070 0.55 +0.7 3,729,663 - PS417_17070 0.56 -0.4 3,729,755 + PS417_17070 0.74 -1.6 3,729,755 + PS417_17070 0.74 -0.8 3,729,755 + PS417_17070 0.74 +1.7 3,730,564 - +0.1 3,730,568 - -1.9 3,730,651 + +0.4 3,730,685 - +0.2 3,730,698 - +3.2 3,730,699 + -0.8 3,730,699 + -0.6 3,730,700 - -0.2 3,730,700 - +0.2 3,730,823 - PS417_17080 0.21 -1.3 3,730,899 + PS417_17080 0.29 +0.3 3,730,899 + PS417_17080 0.29 -2.4 3,730,899 + PS417_17080 0.29 -0.2 3,730,899 + PS417_17080 0.29 +0.0 3,731,082 + +0.1 3,731,083 - PS417_17080 0.48 -0.4 3,731,152 + PS417_17080 0.55 -2.1 3,731,152 + PS417_17080 0.55 -2.8 3,731,153 - PS417_17080 0.55 -0.6 3,731,153 - PS417_17080 0.55 +0.2 3,731,153 - PS417_17080 0.55 -0.7 3,731,166 + PS417_17080 0.56 -1.2 3,731,183 + PS417_17080 0.58 -1.1 3,731,183 + PS417_17080 0.58 -0.3 3,731,184 - PS417_17080 0.58 -0.1 3,731,195 - PS417_17080 0.59 +0.5 3,731,307 - PS417_17080 0.70 -0.8 3,731,322 - PS417_17080 0.72 -0.1 3,731,322 - PS417_17080 0.72 -1.1 3,731,322 - PS417_17080 0.72 -0.0 3,731,383 + PS417_17080 0.78 -0.2 3,731,384 - PS417_17080 0.78 -0.6 3,732,058 + PS417_17085 0.20 -0.8 3,732,059 - PS417_17085 0.20 -0.8 3,732,059 - -1.7 3,732,059 - PS417_17085 0.20 +0.4 3,732,265 - PS417_17085 0.29 -3.0 3,732,300 + PS417_17085 0.30 +0.9 3,732,300 + PS417_17085 0.30 -1.3 3,732,300 + PS417_17085 0.30 -1.7 3,732,301 - PS417_17085 0.30 +0.3 3,732,530 + PS417_17085 0.40 -1.9
Or see this region's nucleotide sequence